Candida morphogenesis and host–pathogen interactions

Whiteway, Malcolm; Oberholzer, Ursula

doi:10.1016/j.mib.2004.06.005

Cited by 175 publications

(134 citation statements)

References 81 publications

Supporting

Mentioning

128

Contrasting

Unclassified

Order By: Relevance

“…Morphological transition in Candida occurs in response to a variety of environmental factors, including amino-acid availability and pH changes 2,12,13 . To determine whether the effects of IL-17A on the fungus were unique to specific experimental settings, cells were exposed to IL-17A under conditions of limited (SPIDER medium) or rich (YPD medium) nutrient availability, nitrogen starvation (SLAD medium) or alkaline pH (M199 medium), in liquid or solid cultures.…”

Section: Il-17a Promotes Fungal Aggregation and Filamentationmentioning

confidence: 99%

Sensing of mammalian IL-17A regulates fungal adaptation and virulence

et al. 2012

View full text Add to dashboard Cite

Section: Il-17a Promotes Fungal Aggregation and Filamentationmentioning

confidence: 99%

Sensing of mammalian IL-17A regulates fungal adaptation and virulence

et al. 2012

View full text Add to dashboard Cite

“…The ability to undergo reversible morphogenetic transitions between yeast, pseudohyphae, and hyphae has been shown to be important for its pathogenicity in systemic infections. Mutants defective in the morphogenetic transition show a much reduced virulence in mouse models of systemic infection (Lo et al, 1997;Whiteway and Oberholzer, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…In C. albicans, several signaling pathways can regulate the yeast-to-hypha transition (Whiteway and Oberholzer, 2004). Among them, the cAMP/PKA pathway plays a major role in hyphal development and virulence, because many mutants in the pathway are defective in hyphal growth and show reduced virulence.…”

Section: Introductionmentioning

confidence: 99%

The Flo8 Transcription Factor Is Essential for Hyphal Development and Virulence inCandida albicans

Cao

Lane

Raniga

et al. 2006

MBoC

198

255

View full text Add to dashboard Cite

The transcription factor Flo8 is essential for filamentous growth in Saccharomyces cerevisiae and is regulated under the cAMP/protein kinase A (PKA) pathway. To determine whether a similar pathway/regulation exists in Candida albicans, we have cloned C. albicans FLO8 by its ability to complement S. cerevisiae flo8. Deleting FLO8 in C. albicans blocked hyphal development and hypha-specific gene expression. The flo8/flo8 mutant is avirulent in a mouse model of systemic infection. Genome-wide transcription profiling of efg1/efg1 and flo8/flo8 using a C. albicans DNA microarray suggests that Flo8 controls subsets of Efg1-regulated genes. Most of these genes are hypha specific, including HGC1 and IHD1. We also show that Flo8 interacts with Efg1 in yeast and hyphal cells by in vivo immunoprecipitation. Similar to efg1/efg1, flo8/flo8 and cdc35/cdc35 show enhanced hyphal growth under an embedded growth condition. Our results suggest that Flo8 may function downstream of the cAMP/PKA pathway, and together with Efg1, regulates the expression of hypha-specific genes and genes that are important for the virulence of C. albicans.

show abstract

“…This morphological transition is thought to be an important virulence factor (Sudbery et al, 2004;Whiteway and Oberholzer, 2004). C. albicans is one of the leading causes of nosocomial bloodstream infections and is capable of causing severe systemic infections (Edmond et al, 1999;Mavor et al, 2005;Spellberg et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…For example, small buds are more likely to disseminate in the bloodstream and hyphal cells may be better suited for invasive growth into tissues. The distinct cell types also differ in their production of virulence factors, such as the adhesin proteins that mediate attachment to host cells and secreted hydrolytic enzymes that facilitate invasive growth (Whiteway and Oberholzer, 2004;Kumamoto and Vinces, 2005).…”

Section: Introductionmentioning

confidence: 99%

Identification of anN-Acetylglucosamine Transporter That Mediates Hyphal Induction inCandida albicans

Álvarez

Konopka

2007

MBoC

125

171

View full text Add to dashboard Cite

The sugar N-acetylglucosamine (GlcNAc) plays an important role in nutrient sensing and cellular regulation in a wide range of organisms from bacteria to humans. In the fungal pathogen Candida albicans, GlcNAc induces a morphological transition from budding to hyphal growth. Proteomic comparison of plasma membrane proteins from buds and from hyphae induced by GlcNAc identified a novel hyphal protein (Ngt1) with similarity to the major facilitator superfamily of transporters. An Ngt1-GFP fusion was detected in the plasma membrane after induction with GlcNAc, but not other related sugars. Ngt1-GFP was also induced by macrophage phagocytosis, suggesting a role for the GlcNAc response in signaling entry into phagolysosomes. NGT1 is needed for efficient GlcNAc uptake and for the ability to induce hyphae at low GlcNAc concentrations. High concentrations of GlcNAc could bypass the need for NGT1 to induce hyphae, indicating that elevated intracellular levels of GlcNAc induce hyphal formation. Expression of NGT1 in Saccharomyces cerevisiae promoted GlcNAc uptake, indicating that Ngt1 acts directly as a GlcNAc transporter. Transport mediated by Ngt1 was specific, as other sugars could not compete for the uptake of GlcNAc. Thus, Ngt1 represents the first eukaryotic GlcNAc transporter to be discovered. The presence of NGT1 homologues in the genome sequences of a wide range of eukaryotes from yeast to mammals suggests that they may also function in the cellular processes regulated by GlcNAc, including those that underlie important diseases such as cancer and diabetes. INTRODUCTIONN-acetylglucosamine (GlcNAc) is an amino sugar that carries out important roles in a broad range of cells from bacteria to humans. One aspect of GlcNAc function is to mediate cellular signaling. In bacteria, GlcNAc induces components that are important for colonization of human hosts, including fimbrins that mediate adhesion to host cells (Sohanpal et al., 2004), multidrug exporter genes (Hirakawa et al., 2006) and Curli fibers that promote biofilm formation (Barnhart et al., 2006). In mammals, GlcNAc is a key sensor of nutrient status that is involved in insulin signaling, cell cycle control, and other essential processes. Nutritional effects that lead to increased GlcNAc levels result in its conversion to UDP-GlcNAc and subsequent attachment to proteins on serine or threonine residues in a dynamic manner that is analogous to modification of proteins by phosphorylation (Slawson and Hart, 2003;Zachara and Hart, 2006). In fact, the interplay between O-GlcNAc modification and phosphorylation of proteins regulates many critical transcription factors, such as c-myc and p53 (Chou and Hart, 2001;Yang et al., 2006). O-GlcNAc modification also regulates other processes including proteosome function (Zachara and Hart, 2004). In addition to these regulatory roles, GlcNAc contributes to the N-linked glycosylation, glycophosphatidylinositol (GPI) anchor addition to proteins and is polymerized into chitin, which forms part of the fungal cell wall and the exoskele...

show abstract

Candida morphogenesis and host–pathogen interactions

Cited by 175 publications

References 81 publications

Sensing of mammalian IL-17A regulates fungal adaptation and virulence

Sensing of mammalian IL-17A regulates fungal adaptation and virulence

The Flo8 Transcription Factor Is Essential for Hyphal Development and Virulence inCandida albicans

Identification of anN-Acetylglucosamine Transporter That Mediates Hyphal Induction inCandida albicans

Contact Info

Product

Resources

About