Candida konsanensis sp. nov., a new yeast species isolated from Jasminum adenophyllum in Thailand with potentially carboxymethyl cellulase-producing capability

Abstract

A new yeast species (KKU-FW10) belonging to the Candida genus was isolated from Jasminum adenophyllum in the Plant Genetic Conservation Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn area, Chulabhorn Dam, Konsan district within Chaiyaphum province in Thailand. The strain was identified via analysis of nucleotide sequences from the D1/D2 domain of 26S ribosomal DNA and based on its morphological, physiological and biochemical characteristics. The sequence obtained from yeast isolate KKU-FW10 was 97 percent identical to that of Candida chanthaburiensis (GenBank accession number AB500861.1), with 506/517 (nucleotides identity/total nucleotides) matching nucleotides, nine substitutions and two gaps being detected. This species belonged to the Candida clade. Regarding morphological characteristics, isolate KKU-FW10 presents cream-colored butyrous colonies, vegetative reproduction through budding and, round cells without filaments or ascospores. The major ubiquinone detected was Q-9. The above results suggest that isolate KKU-FW10 is a new member of the genus Candida, and the name Candida konsanensis is proposed for this yeast. The type strain of the new species is KKU-FW10(T) (= BCC 52588(T), = NBRC 109082(T), = CBS 12666(T)). In addition, this KKU-FW10 could potentially produce 58.24 Units/ml of carboxymethyl cellulase when it was cultured in YP broth containing 1.0 % carboxymethyl cellulose for 24 h.

Candida metrosideri pro tempore sp. nov. and Candida ohialehuae pro tempore sp. nov., two antifungal-resistant yeasts associated with Metrosideros polymorpha flowers in Hawaii

“… Strains: 1, Candida vulturna CBS 14366 T ; Candida doubushaemulonii CBS 7798 T ; 3, Candida pseudohaemulonii CBS 10004 T ; 4, Candida heveicola CBS 10702 T ; 5, Candida konsanensis CBS 12666 T ; 6, Candida chantaburiensis CBS 10926 T ; 7, Candida metrosideri JK22 T ; 8, Candida ohialehuae JK58.2 T . Data for the reference strains originates from references [ 16 , 20 , 21 , 47 , 48 ] and the CBS database ( http://www.cbs.knaw.nl ). All isolates were positive for assimilation of D-glucose, sucrose, trehalose, maltose, glycerol, D-mannitol, D-glucitol, and L-lysine, and negative for assimilation of lactose.…”

“…chantaburiensis [ 47 ], C . konsanensis [ 48 ], and C . vulturna [ 20 ]), it can be hypothesized that these yeasts may be adapted to efficient growth on plant surfaces.…”

“…Tested isolates of C. metrosideri and C. ohialehuae did not display any hemolytic activity and were unable to grow at 37˚C. In contrast, closely-related species such as C. auris, C. ruelliae, C. haemulonis, C. duobushaemulonis, C. pseudohaemulonis, C. vulturna, C. heveicola, C. chantaburiensis, and C. konsanensis can grow at 37˚C, and most of them have been repeatedly isolated from clinical samples [16,[19][20][21][46][47][48][49]. However, based on the study performed we cannot discard the possibility that C. ohialehuae and C. metrosideri might act as opportunistic pathogens of humans and/or animals that have niches outside clinical and endothermic environments in which they can thrive [50].…”

“…As both C. metrosideri and C. ohialehuae were isolated from floral parts (stamens and styles, respectively) and most of their close phylogenetic relatives were originally found in plant material of tropical regions near the Pacific ocean (e.g. C. ruelliae [19], C. heveicola [49], C. chantaburiensis [47], C. konsanensis [48], and C. vulturna [20]), it can be hypothesized that these yeasts may be adapted to efficient growth on plant surfaces. In this regard, the phenotypic assays carried out in this study confirmed that both C. metrosideri and C. ohialehuae can assimilate the three main simple sugars present in floral nectar and other plant secretions (i.e.…”

Yeasts from peat in a tropical peat swamp forest in Thailand and their ability to produce ethanol, indole-3-acetic acid and extracellular enzymes

“…In this study, all strains failed to produce exoglucanase. This may reflect the fact that we tested only 65 strains of ten species, and few yeast species have been found to produce exoglucanase (Sarawan et al 2013). Some strains of Cu.…”