Candida glabrata displays pseudohyphal growth

Csank, Csilla

doi:10.1016/s0378-1097(00)00241-x

Cited by 44 publications

(56 citation statements)

References 24 publications

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“…On agar, colonies grown for 5 days at 25 u C contained approximately the same number of cells, and were visually of similar size, suggesting similar rates of cell multiplication. IWr colonies contained lower concentrations, primarily as a result of a significant proportion of pseudohyphal growth (Lachke et al, 2002;Csank & Haynes, 2000). In liquid culture at 25 u C, the generation times for DB, LB and Wh cells were 1.7, 1.8 and 1.9 h, respectively.…”

Section: Characterization Of the Cell Preparations Used In Animal Modmentioning

confidence: 99%

Dark brown is the more virulent of the switch phenotypes of Candida glabrata

Srikantha

Daniels

et al. 2008

Microbiology

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Candida glabrata undergoes reversible, high-frequency core switching between phenotypes that include dark brown (DB), light brown (LB) and white (Wh). These phenotypes in turn can switch to the irregular wrinkle (IWr) phenotype. Natural isolates, however, express predominantly the DB phenotype, leading to the hypothesis that it has a colonization advantage over the other switch phenotypes. Using the mouse model of systemic infection, results are presented which support this hypothesis. DB has an advantage over other switch phenotypes in colonizing the two major target organs in the mouse model, the spleen and liver. A time-course study reveals that colonization of the major target organs occurs very rapidly (within 2 h) after host injection, and that the DB advantage for spleen and liver colonization is immediate. The DB advantage is maintained during clearing from spleen, liver and kidneys, and during delayed transient brain colonization. These results demonstrate that DB has a colonization advantage over other switch phenotypes, and that the switch phenotype expressed by a colonizing population therefore plays a fundamental role in virulence. It is therefore essential that switching be considered in both in vivo and in vitro studies of C. glabrata virulence.

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Section: Characterization Of the Cell Preparations Used In Animal Modmentioning

confidence: 99%

Dark brown is the more virulent of the switch phenotypes of Candida glabrata

Srikantha

Daniels

et al. 2008

Microbiology

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“…glabrata is known to switch to pseudohyphal growth when starved of nitrogen (Csank & Haynes, 2000), but the fungus does not form hyphae during host invasion (Lachke et al, 2002) and only budding growth has been observed in animal tissues (Fidel et al, 1999). In this study, highly FLresistant C. glabrata mutants formed a higher number of budding yeasts and also larger aggregates than the parental control when cultured in FCS, indicating that this attribute may be associated with high FL resistance.…”

Section: Induction Of Yeast Bud Formation and Co-aggregationmentioning

confidence: 99%

Fluconazole resistance in Candida glabrata is associated with increased bud formation and metallothionein production

Samaranayake

Cheung

Wang

et al. 2013

Journal of Medical Microbiology

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Virulence associated with fluconazole (FL) resistance in Candida glabrata is a global problem and has not been well characterized at the proteome level. In this study, a stable FL-resistant (MIC .256 mg ml "1 ) strain of C. glabrata was generated on agar containing FL. Eight phenotypic mutants were characterized by contour-clamped homogeneous electrophoretic field analysis and two-dimensional PAGE. The secondary derivatives of C. glabrata yielded four distinct genotypes with varying chromosomal profiles. Proteomic analysis performed by tandem mass spectrometry for two of the mutants, CG L2 and CG S3 , demonstrated a total of 25 differentially regulated proteins of which 13 were upregulated and 12 were downregulated or were similar compared with the parental isolate. The mRNA transcript levels of significantly (P,0.001) upregulated genes were determined by quantitative RT-PCR analysis, and their physiological relevance in terms of phenotypic expression of virulence attributes was verified by conventional laboratory methodologies. The data showed that the FL resistance (MIC .256 mg ml "1 ) of CG S3 was associated with significantly upregulated (P,0.001) mRNA transcript levels of several genes -ERG11, CDR1, CDR2, MFS, MTI, TPR, VPS and EFT2 -in addition to a number of other potentially virulent genes expressed differentially at a lower level. The results demonstrated accentuated phenotypic expression of bud formation of yeast and metallothionein production associated with FL resistance in C. glabrata, which may help the fungus to colonize the host.

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“…In both nonpathogenic (Saccharomyces cerevisiae) and pathogenic (Candida albicans, Candida glabrata, and Cryptococcus neoformans) fungi, the ability to switch between the yeast and filamentous form is dependent on environmental conditions. All of these organisms switch from the yeast to the filamentous form when starved for nitrogen (Gimeno et al 1992;Wickes et al 1996;Csank and Haynes 2000;Biswas and Morschhauser 2005). In S. cerevisiae, nitrogen starvation induces pseudohyphal growth in diploid cells (Gimeno et al 1992) and enhances invasive growth in haploid cells cultured on synthetic minimal medium (H. Chen and G. Fink, unpubl.).…”

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confidence: 99%

Feedback control of morphogenesis in fungi by aromatic alcohols

Chen¹,

Fink²

2006

Genes Dev.

403

445

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Many fungi undergo a developmental transition from a unicellular yeast form to an invasive filamentous form in response to environmental cues. Here we describe a quorum signaling pathway that links environmental sensing to morphogenesis in Saccharomyces cerevisiae. Saccharomyces cells secrete aromatic alcohols that stimulate morphogenesis by inducing the expression of FLO11 through a Tpk2p-dependent mechanism. Mutants defective in synthesis of these alcohols show reduced filamentous growth, which is partially suppressed by the addition of these aromatic alcohols. The production of these autosignaling alcohols is regulated by nitrogen: High ammonia restricts it by repressing the expression of their biosynthetic pathway, whereas nitrogen-poor conditions activate it. Moreover, the production of these aromatic alcohols is controlled by cell density and subjected to positive feedback regulation, which requires the transcription factor Aro80p. These interactions define a quorum-sensing circuit that allows Saccharomyces to respond to both cell density and the nutritional state of the environment. These same autoregulatory molecules do not evoke the morphological switch in Candida albicans, suggesting that these molecular signals are species-specific.[Keywords: Aromatic alcohols; morphogenesis; quorum sensing; fungi] Supplemental material is available at http://www.genesdev.org.

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Candida glabrata displays pseudohyphal growth

Cited by 44 publications

References 24 publications

Dark brown is the more virulent of the switch phenotypes of Candida glabrata

Dark brown is the more virulent of the switch phenotypes of Candida glabrata

Fluconazole resistance in Candida glabrata is associated with increased bud formation and metallothionein production

Feedback control of morphogenesis in fungi by aromatic alcohols

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