Candida auris in contemporary mycology labs: A few practical tricks to identify it reliably according to one recent French experience

Abstract

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A New Age in Molecular Diagnostics for Invasive Fungal Disease: Are We Ready?

“…Increased prevalence, in particular of Candida glabrata infections including those due to azole-resistant, or echinocandin-azole co-resistant isolates, as well as multi-azole resistant Candida tropicalis isolates has been noted (Pfaller et al, 2012;Chapman et al, 2017). Of added concern is the global emergence of multi-drug resistant fungal species, including Candida auris (Desoubeaux et al, 2018), pan-azole resistant Aspergillus fumigatus driven by agricultural triazole use (Snelders et al, 2009;van der Linden et al, 2013;Navalkele et al, 2017), as well as rare molds which are often resistant to most if not all antifungal drugs (Malani and Kauffman, 2007;Dellière et al, 2019).…”

Diagnosis, management and prevention of Candida auris in hospitals: position statement of the Australasian Society for Infectious Diseases

“…2). 33,35,36 Chromogenic agars cannot be used for primary identification, but are useful to highlight colonies suspicious for C. auris, especially from mixed cultures.…”

“…On CHROMagar Candida (Becton Dickinson & Company, Baltimore, MD, USA) or Agar Candida ID2 (CAN2) medium (bioMerieux, Marcy l'Etoile, France) colonies are beige/white, then light pink to red or purple by day 4 of growth (Fig. ) . Chromogenic agars cannot be used for primary identification, but are useful to highlight colonies suspicious for C .…”

“…Common misidentifications of Candida auris by commercial phenotypic identification systems18,35,37 Long read sequencing has been used to investigate hospital outbreaks 20,53. Although WGS can offer a shorter turnaround time (8-72 h) than other typing methods, it requires extra bioinformatic expertise and is more expensive.…”

Candida auris : une nouvelle menace pour les patients de réanimation ?

“…Aucun cas secondaire n'avait été rapporté à partir de ces deux cas historiques. Plus récemment, une colonisation a été détectée en 2017 à Tours chez un patient de 58 ans hospitalisé pour transplantation hépatique [9]. Ce patient, préalablement hospitalisé en Iran et en Inde, était porteur de bactéries multirésistantes et faisait l'objet d'une surveillance microbiologique.…”