Candida auris biofilm: a review on model to mechanism conservation

A Novel Robust Method Mimicking Human Substratum To Dissect the Heterogeneity of Candida auris Biofilm Formation

“…Gelatin is an economical alternative to Thermanox coverslips, as it mimics the in vivo system of the host closely. We have assessed the prevailing techniques to understand the biofilm-forming capability of gelatin ( 16 ). We have also incorporated a technique to measure biofilm thickness using microscopy that could reduce handling errors.…”

“…RPMI 1640 medium was used to form biofilm to mimic other in vivo conditions ( 23 ). This is important, particularly in the case of C. auris , where it shows different growth phenotypes in different conditions ( 13 , 15 , 16 ). To differentiate the biofilm formation in two different conditions, we grew C. auris biofilm in both modified synthetic sweat medium and RPMI 1640 on a gelatin-coated coverslip and monitored them by scanning electron microscopy.…”

“…For example, Sherry et al ( 14 ) and Horton et al ( 15 ) describe the architecture of C. auris biofilm grown in vitro on a Thermanox (polystyrene) coverslip consisting of a single layer of yeast cells, which is not the typical biofilm architecture ( 14 , 15 ). While Horton et al further described the use of porcine skin surface and synthetic sweat media to proliferate multilayered biofilm ( 15 , 16 ), these available in vitro methods lack cost-effectiveness, especially in the case of the large-scale screening of libraries.…”

First report of Candida auris in Guangdong, China: clinical and microbiological characteristics of 7 episodes of candidemia

“…C. auris is notorious for its persistence in the environment due to some factors such as biofilm formation, thermoresistant capacity and partial resistance to commonly used disinfectants [ 1 , 5 ]. Detecting biofilm production is important since biofilms play an important role in catheter-associated candidemia [ 53 ]. Invasive candidiasis, especially catheter-associated candidemia, is thought to originate after dispersion and subsequent hematogenous dissemination from a biofilm formed inside a catheter [ 54 ].…”

Mining the nanotube-forming Bacillus amyloliquefaciens MR14M3 genome for determining anti-Candida auris and anti-Candida albicans potential by pathogenicity and comparative genomics analysis

“…Since its discovery in 2009 in Japan, the multidrug-resistant (MDR) fungus Candida auris has gained international attention as an emerging threat and a challenging fungal pathogen to treat [1] , [2] , [3] , [4] , [5] , [6] . It displays significant innate and acquired resistance to a variety of antifungal drugs [3] .…”