Candida albicans infections in actively and passively immunized animals

Hurd, R. C.; Drake, Charles H.

doi:10.1007/bf02056710

Cited by 30 publications

(12 citation statements)

References 7 publications

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“…However, this is, to our knowledge, the first demonstration that a nonprotective anti-Candida serum becomes protective against a lethal systemic challenge when deprived of some antibodies. Our observation may explain why anti-Candida sera have been so inconsistent in transferring protection and why immunization with whole inactivated cells of C. albicans has been so variably protective though always stimulating a delayed-type hypersensitivity reaction, other CMI responses, and abundant anti-Candida antibodies (23,33,44). Our data strongly suggest that antibody-mediated protection against C. albicans not only requires the presence of the right antibody but also requires the absence of other antibodies.…”

Section: Discussionmentioning

confidence: 62%

Interplay between Protective and Inhibitory Antibodies Dictates the Outcome of Experimentally Disseminated Candidiasis in Recipients of aCandida albicansVaccine

et al. 2002

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Mice immunized with heat-inactivated, whole yeast-form cells (Y cells) ofCandida albicans developed intense, specific humoral and cell-mediated immune responses. However, they were modestly protected against a lethal challenge by the fungus, and their sera did not confer passive protection upon nonimmunized animals. Surprisingly, this immune serum conferred an elevated degree of passive protection to normal and SCID mice when preadsorbed on whole C. albicans cells. After adsorption, no antibodies specific to mannoprotein (MP)-rich extracts or secretions were detected by indirect enzyme-linked immunosorbent assay and no serum reaction with the fungal cell surface was seen in immunofluorescence assays. However, this serum had totally preserved the level of other antibodies, in particular those reacting with ␤-1,3 and ␤-1,6 glucan (GG). The hypothesis that anti-GG antibodies contributed to the passive protection was suggested by the following circumstantial evidence: (i) mice immunized with C. albicans cells treated with dithiothreitol and protease (YDP cells), which exposed GG on their surfaces and generated anti-GG but not anti-MP antibodies, were substantially protected against a lethal fungus challenge; (ii) the sera, and their immunoglobulin fractions, of mice immunized with YDP cells transferred protection to nonimmune animals; and (iii) this passive protection was substantially abolished by preadsorption on GG but not on intact cells. Overall, our findings demonstrate that some anti-Candida antibodies can block the protective potential of immune serum, a potential to which anti-GG antibodies appear to contribute. Our observations may also help explain why subjects with elevated anti-Candida antibody titers, inclusive of anti-MP and anti-GG antibodies, remain nonetheless susceptible to invasive candidiasis.

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Section: Discussionmentioning

confidence: 62%

Interplay between Protective and Inhibitory Antibodies Dictates the Outcome of Experimentally Disseminated Candidiasis in Recipients of aCandida albicansVaccine

et al. 2002

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“…Much of the confusion stems from earlier studies that concluded antibodies were dispensable for resolution of fungal infections (Hurd and Drake 1953;Goren 1967;Diamond et al 1974;Monga et al 1979;Carrow et al 1984). However, the advent of monoclonal antibody technology made it possible to identify the protective effects of immunoglobulins against fungi.…”

Section: Humoral Immunitymentioning

confidence: 99%

Adaptive Immunity to Fungi

Verma

Wüthrich

Deepe

et al. 2014

Cold Spring Harbor Perspectives in Medicine

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“…Hurd & Drake (1953) in experiments designed to demonstrate passive immunity in Candida infections, actively immunized rabbits with a long series of inoculations using formalin-killed C. albicans. The sera of 2 rabbits so treated for 9 months had agglutination titers of 1:1280 and 1:2560, but their sera did not passively protect normal animals.…”

Section: Resultsmentioning

confidence: 99%