Candida albicans forms biofilms on the vaginal mucosa

Harriott, Melphine; Lilly, Elizabeth A.; Rodriguez, Tomas; Fidel, Paul L.; Noverr, Mairi C.

doi:10.1099/mic.0.039354-0

Cited by 257 publications

(258 citation statements)

References 36 publications

(61 reference statements)

Supporting

Mentioning

248

Contrasting

Unclassified

Order By: Relevance

“…94 Transcriptional regulation of biofilm formation has been mainly attributed to Bcr1, Tec1, and Efg1, 90 however, recent studies revealed novel transcription factors associated with this process: Ndt80, Rob1, Brg1. 95 Harriott et al 96 were the first to show using in vivo and ex vivo models of murine Candida vaginitis that C. albicans does, indeed, form Bcr1-and Efg1-dependent biofilms. Similar to other body sites, biofilms in the vagina are of major concern, as they have been implicated in immunopathology of VVC, anti-fungal treatment failures and recurrent infections.…”

Section: Pathogenesis Of Candida and Vulvovaginal Candidiasismentioning

confidence: 99%

The vaginal mycobiome: A contemporary perspective on fungi in women's health and diseases

2016

View full text Add to dashboard Cite

Most of what is known about fungi in the human vagina has come from culture-based studies and phenotypic characterization of single organisms. Though valuable, these approaches have masked the complexity of fungal communities within the vagina. The vaginal mycobiome has become an emerging field of study as genomics tools are increasingly employed and we begin to appreciate the role these fungal communities play in human health and disease. Though vastly outnumbered by its bacterial counterparts, fungi are important constituents of the vaginal ecosystem in many healthy women. Candida albicans, an opportunistic fungal pathogen, colonizes 20% of women without causing any overt symptoms, yet it is one of the leading causes of infectious vaginitis. Understanding its mechanisms of commensalism and pathogenesis are both essential to developing more effective therapies. Describing the interactions between Candida, bacteria (such as Lactobacillus spp.) and other fungi in the vagina is fundamental to our characterization of the vaginal mycobiome.

show abstract

Section: Pathogenesis Of Candida and Vulvovaginal Candidiasismentioning

confidence: 99%

The vaginal mycobiome: A contemporary perspective on fungi in women's health and diseases

2016

View full text Add to dashboard Cite

show abstract

“…As an initial test of filastatin in a mammalian infection model, we examined a mouse model of vulvovaginal candidiasis (VVC). This system is ideal for evaluating our compounds, because VVC infections depend on fungal morphogenesis and biofilm formation (35). This assay is medically important, because VVC affects 75% of all women at least once in their lifetimes, and because C. albicans is responsible for 85-95% of these infections (36).…”

Section: Filastatin Alters Biofilm Morphology In a Mouse Model Of Vulmentioning

confidence: 99%

Chemical screening identifies filastatin, a small molecule inhibitor of Candida albicans adhesion, morphogenesis, and pathogenesis

Fazly¹,

Jain

Dehner³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

100

View full text Add to dashboard Cite

Infection by pathogenic fungi, such as Candida albicans, begins with adhesion to host cells or implanted medical devices followed by biofilm formation. By high-throughput phenotypic screening of small molecules, we identified compounds that inhibit adhesion of C. albicans to polystyrene. Our lead candidate compound also inhibits binding of C. albicans to cultured human epithelial cells, the yeast-to-hyphal morphological transition, induction of the hyphal-specific HWP1 promoter, biofilm formation on silicone elastomers, and pathogenesis in a nematode infection model as well as alters fungal morphology in a mouse mucosal infection assay. We term this compound filastatin based on its strong inhibition of filamentation, and we use chemical genetic experiments to show that it acts downstream of multiple signaling pathways. These studies show that high-throughput functional assays targeting fungal adhesion can provide chemical probes for study of multiple aspects of fungal pathogenesis.hyphal morphogenesis | small molecule screening | fungal pathogens

show abstract

“…Other Candida spp. including C. tropicalis, C. parapsilosis, and C. glabrata form ECM-containing biofilms but do not produce true hyphae [18]. Aspergillus biofilms can form both on abiotic and biotic surfaces [19].…”

Section: Fungal Biofilmsmentioning

confidence: 99%

Role of Biofilms in Fungal Keratitis- An Overview

Sharma

Singh

Goel

et al. 2017

JBMOA

View full text Add to dashboard Cite

Biofilms are generally defined as a community of sessile microbes held together by a polymeric extracellular matrix (ECM), adherent to a surface that are phenotypic ally distinct from their plank tonic counterparts. Fungal keratitis is an important cause of ocular morbidity in the form of corneal opacity and visual loss, especially in developing countries. Predisposing factors for fungal keratitis include tropical or subtropical climate, agricultural work, contact lens users and corneal trauma. Fungal keratitis is most commonly caused by filamentous fungi like Fusarium spp. (F. solani and F. oxysporum) followed by Aspergillus spp. and dematiaceous fungi. Fungal biofilm formation plays an important role in the development of fungal keratitis. Here in this review, we will be discussing the formation of biofilms and its role in fungal keratitis. Keywords: Fungal KeratitisFungal keratitis is an important cause of ocular morbidity in the form of corneal opacity and visual loss especially in developing countries, where it may account for nearly half of corneal ulcers [23]. Predisposing factors for fungal keratitis include tropical or subtropical climate, agricultural work, contact lens users and corneal trauma. Fungal keratitis is most commonly caused by filamentous fungi like Fusarium spp. (F. solani and F. oxysporonoxysporum) followed by Aspergillus spp. and dematiaceous fungi [23,24] (Figure 2). Contact Lens-Associated Fungal KeratitisHigh risk of fungal keratitis in contact lens wearers has been associated with the ability of the lens to induce modification of the corneal epithelium and to carry organisms to the ocular surface [25,26]. Use of lens induces local alterations like hypoxia and hypercapnia, which affect the ability of the epithelium to respond to damage. Tear fluid exchange may also be compromised between the anterior and posterior sides of the lens thereby limiting its antimicrobial properties [25]. In addition, contact lenses provide a surface where microorganisms may attach and colonize the surface as a biofilm and can spread to a previously damaged corneal epithelium [26]. Poor hygiene and infrequent replacement of the contact lens storage cases are independent risk factors for moderate and sever keratitis [27]. The incidence of contact lens-associated microbial keratitis has been shown to be impacted by thecontact lens material, and also by the wear schedule. Higher risk for development of keratitis was seen with daily wear soft contact lenses compared to daily wear rigid gas permeable lenses, and it was further increased for extended wear (overnight wear) soft contact lenses [28,29]. Recently introduced daily disposable and silicone hydrogel contact lenses have also been associated with higher incidence of keratitis compared to rigid gas permeable contact lenses [30,31]. Role of Contact Lenses in Biofilm FormationOnce the lens is placed on the surface of the eye, the disinfectant diffuses off the lens and is replaced by lipids and proteins present in the tear fluid [32]. Microbial adh...

show abstract

Candida albicans forms biofilms on the vaginal mucosa

Cited by 257 publications

References 36 publications

The vaginal mycobiome: A contemporary perspective on fungi in women's health and diseases

The vaginal mycobiome: A contemporary perspective on fungi in women's health and diseases

Chemical screening identifies filastatin, a small molecule inhibitor of Candida albicans adhesion, morphogenesis, and pathogenesis

Role of Biofilms in Fungal Keratitis- An Overview

Contact Info

Product

Resources

About