Candida albicans biofilm heterogeneity does not influence denture stomatitis but strongly influences denture cleansing capacity

O’Donnell, Lindsay E.; Alalwan, Hasanain K.A.; Kean, Ryan; Calvert, Gareth; Nile, Christopher J.; Lappin, David F.; Robertson, Douglas; Williams, Craig; Ramage, Gordon; Sherry, Leighann

doi:10.1099/jmm.0.000419

Cited by 24 publications

(19 citation statements)

References 46 publications

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“…Poor oral hygiene is frequently observed within this patient group and several factors can impact the onset of DS such as salivary flow, denture cleanliness, age of denture, smoking, and diet [85]. Soft tissue inflammation below or above the denture, as a result of persistent exposure to microorganisms, is characteristic of DS [86]. Microbes frequently adhere to the denture surface and a biofilm quickly develops which can contain numerous species of bacteria and fungi.…”

Section: Denture Stomatitis: Mixing In a Rough Neighbourhoodmentioning

confidence: 99%

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Delaney

Kean

Short

et al. 2018

Curr Clin Micro Rpt

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Purpose of Review Over the last decade, microbiome studies have enhanced our knowledge and understanding of the polymicrobial nature of oral infections. Recently, profiling of the fungal microbiome has expanded our conventional understanding of oral ecology, revealing the critical importance of yeasts within this complex microbiome. This review aims to explore our current appreciation of interkingdom interactions in oral disease. Recent Findings There is a growing evidence base of interactions and pathogenic synergy and antagonism with bacterial species within oral disease. Recent studies have helped to develop our knowledge of how Candida albicans, alongside bacteria such as Porphyromonas gingivalis, Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, and Lactobacillus species, influence overall pathogenicity. Summary Clinical and experimental evidence makes a compelling case for a role for C. albicans in a number of oral infections, though whether its role is an active accomplice or passive bystander remains to be determined.

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Section: Denture Stomatitis: Mixing In a Rough Neighbourhoodmentioning

confidence: 99%

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Delaney

Kean

Short

et al. 2018

Curr Clin Micro Rpt

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“…This is important because, according to the literature, although patients realise the importance of cleaning a prosthesis, they may not necessarily know how to do it. 16 In addition, in patients with systemic disorders, candidiasis is more often found in the oral cavity compared to healthy people. 17 Polished resins that have a coating of silicon dioxide showed the lowest amount of C. albicans compared to polished resins and resins without the application of coating, with sig n ificant differences between the RPC and RP groups.…”

Section: Discussionmentioning

confidence: 99%

Effect of Silicon dioxide coating of acrylic resin surfaces on Candida albicans adhesion

et al. 2020

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Acrylic resin has been used in the manufacture of prostheses, however, in the oral cavity, this material starts to retain microorganisms capable of causing gingival inflammation due its porosities. The aim of this study was to evaluate the influence of the use of silicon dioxide as a coating layer applied onto acrylic resin, on the adhesion of Candida albicans (Ca). After the incubation period in Sabouraud Dextrose Broth, a total of 1 ml of the Ca suspension was added to plate wells, each well containing a specimen of acrylic resin. The adhesion ability of Ca on acrylic resin was determined by counting colonies. Three groups (n = 6) of acrylic resin were assessed: with polishing (RP); without polishing (RW); with polishing and coating layer of silicon dioxide (RPC). Ca deposited on the surface of the acrylic resin was also observed using Scanning Electron Microscopy (SEM). Statistical assessment by Kruskal-Wallis and Student-Newman-Keuls Method were done (α = 2%). There was significant difference among the groups. The RPC group showed the lowest growth, with an average of 5.59 Log CFU/cm²; there was a statistically significant difference in relation to group RW, which presented a growth of 6.07 Log CFU/cm² and to group RP with 5.91 Log CFU/cm 2 (p < 000.1). SEM images demonstrated that in the RP and RPC group, the surface of the resin had greater regularity, and smaller number of microorganisms. The application of silicon dioxide coating on acrylic resin appears to be a promising alternative, and its use can help in reducing the adhesion of Ca in prostheses.

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“…The oral and vaginal epithelium provide a mucosal niche for biofilm formation, whilst indwelling medical devices such as prosthetic heart valves and central venous catheters provide an inert, abiotic substrate for subsequent biofilm adherence and proliferation [ 20 , 21 ]. Irrespective of isolation site, biofilm heterogeneity has been reported, including the oral cavity, bloodstream, and urinary tract [ 14 , 22 , 23 , 24 , 25 , 26 ].…”

Section: Is Heterogeneity Clinically Important?mentioning

confidence: 99%

Gaining Insights from Candida Biofilm Heterogeneity: One Size Does Not Fit All

Kean

Delaney

Rajendran

et al. 2018

JoF

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Despite their clinical significance and substantial human health burden, fungal infections remain relatively under-appreciated. The widespread overuse of antibiotics and the increasing requirement for indwelling medical devices provides an opportunistic potential for the overgrowth and colonization of pathogenic Candida species on both biological and inert substrates. Indeed, it is now widely recognized that biofilms are a highly important part of their virulence repertoire. Candida albicans is regarded as the primary fungal biofilm forming species, yet there is also increasing interest and growing body of evidence for non-Candida albicans species (NCAS) biofilms, and interkingdom biofilm interactions. C. albicans biofilms are heterogeneous structures by definition, existing as three-dimensional populations of yeast, pseudo-hyphae, and hyphae, embedded within a self-produced extracellular matrix. Classical molecular approaches, driven by extensive studies of laboratory strains and mutants, have enhanced our knowledge and understanding of how these complex communities develop, thrive, and cause host-mediated damage. Yet our clinical observations tell a different story, with differential patient responses potentially due to inherent biological heterogeneity from specific clinical isolates associated with their infections. This review explores some of the recent advances made in an attempt to explore the importance of working with clinical isolates, and what this has taught us.

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Candida albicans biofilm heterogeneity does not influence denture stomatitis but strongly influences denture cleansing capacity

Cited by 24 publications

References 46 publications

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Fungi at the Scene of the Crime: Innocent Bystanders or Accomplices in Oral Infections?

Effect of Silicon dioxide coating of acrylic resin surfaces on Candida albicans adhesion

Gaining Insights from Candida Biofilm Heterogeneity: One Size Does Not Fit All

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