Candida albicans adherence on silicone elastomers: Effect of polymerisation duration and exposure to simulated saliva and nasal secretion

Kurtulmuş, Hüseyin; Kümbüloğlu, Övül; Özcan, Mutlu; Özdemir, Güven; Vural, Caner

doi:10.1016/j.dental.2009.09.001

Cited by 20 publications

(17 citation statements)

References 35 publications

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“…Denture base materials are also expected to be biocompatible and durable in the oral cavity environment [2] but such materials are subject of various unfavorable conditions in the oral cavity that may lead to alterations on their chemical, physical and mechanical properties [1,8]. Furthermore, surface properties of soft lining materials may contribute to colonization of microorganisms because loss of substances such as plasticizers may lead to surface roughness as a result of loss of elasticity [2,[9][10][11]. Surface roughness in particular usually causes irregular micropores and microorganisms adhering to these surfaces are sheltered from shear forces and cleaning procedures [11].…”

Section: O R I G I N a L A R T I C L Ementioning

confidence: 99%

Prevalence of Candida albicans and Streptococcus aureus on maxillary obturators, maxillary defects and in saliva: A cross-sectional study

Çelakıl

Baca

Topçuoğlu

et al. 2017

BDS

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Objective: This study investigated on the incidence of Candida albicans and Staphylococcus aureus adhesion onto maxillary obturator prostheses with soft lining materials placed by prosthodontists. Material and Methods: Between years 2013 and 2014, patients with maxillary defects due to maxillary tumor surgery and acquired obturator prostheses (n = 21, study group) were clinically evaluated and microbiological outcomes were compared with complete maxillary denture wearers (n = 21, control group). Data were collected on demographic features, histological type of cancer, presence of chemotherapy or radiotherapy, identification of soft lining materials used on obturator prostheses, cleansing methods practiced for obturator and maxillary complete prostheses. The presence of Candida albicans was determined on soft lining materials or polymethylmethacrylate surfaces and in the oral cavity, and Staphylococcus aureus in nasal mucosa and saliva. Data were analyzed using Kruskal Wallis, Fisher-Freeman-Halton tests (p < 0.05). Results: The highest amount of Candida albicans was found on surfaces of prostheses, in the saliva and on maxillary defects on the hard palate and on definitive soft lining material with 90%, 90%, and 70%, respectively. The highest amount of Staphylococcus aureus was observed in the saliva and nasal cavity in the definitive soft lining material group (50%). Conclusion: Short renewal period of lining materials may cause less Candida albicans colonisation and denture stomatitis in the oral cavity. Patients who have undergone maxillectomy were more prone to Staphylococcus aureus colonisation.KeywordsCandida albicans; Denture liners; Maxillary prosthesis; Palatal obturators; Staphylococcus aureus.

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Section: O R I G I N a L A R T I C L Ementioning

confidence: 99%

Prevalence of Candida albicans and Streptococcus aureus on maxillary obturators, maxillary defects and in saliva: A cross-sectional study

Çelakıl

Baca

Topçuoğlu

et al. 2017

BDS

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“…The prosthesis needs to be biocompatible to enable wound healing and the restoration of healthy tissue; but the material also needs to be aesthetically acceptable to the patient . Maxillofacial prostheses are exposed to saliva and nasal secretions and thus they are inevitably susceptible to bacterial colonization, which usually leads to the subsequent degradation of the material and infection of the surrounding tissues . There is a wide range of microbial species that are known to colonize the biomaterials used for prostheses …”

Section: Introductionmentioning

confidence: 99%

Antifungal properties and biocompatibility of silver nanoparticle coatings on silicone maxillofacial prostheses in vitro

et al. 2017

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Patients with facial prostheses suffer from yeast, Candida albicans, infections. This study aimed to determine the biocompatibility and antifungal properties of silicone facial prostheses coated with silver nanoparticles (Ag NPs) in vitro. Medical grade silicone discs were coated with 5 and 50 mg L dispersions of either Ag NPs or AgNO . Coatings were fully characterized using scanning electron microscopy and energy dispersive X-ray spectroscopy. The biocompatibility was examined using human dermal fibroblasts (Hs68), whereas antifungal efficacy was tested against C. albicans (NCPF-3179). The fibroblast viability was assessed by measuring lactate dehydrogenase (LDH) activity, protein content and tissue electrolytes. There were no effects on the LDH activity of fibroblast cell homogenates, and leak of LDH activity into external media remained low (0.1-0.2 IU mL ). Sublethal effects of Ag NP coatings on membrane permeability/ion balance was not observed, as measured by stable homogenate Na and K concentrations. Some Ag (13 mg L ) was detected from the AgNO coatings in the media, but total Ag remained below detection limit (<1.2 µg L ) for the Ag NP coatings; indicating the latter were stable. When fibroblasts grown on silver coatings were challenged with C. albicans, the Ag NP coating was effective at preventing fungal growth as measured by ethanol production by the yeast, and without damaging the fibroblasts. Ethanol production decreased from 43.2 ± 25.02 in controls to 3.6 µmol mL in all the silver treatments. Data shows that silicone prosthetic materials coated with Ag NPs are biocompatible with fibroblast cells in vitro and show antifungal properties. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1038-1051, 2018.

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“…Silicone, with its excellent biocompatibility and biodurability properties, has been widely used for implantable biomedical devices such as catheters (Bayston et al, 2009; Carr, 2000; Stevens et al, 2009), stents (Chew and Denstedt, 2004; Chew and Lange, 2009; Venkatesan et al, 2010), maxilla‐facial (Kurtulmus et al, 2010), and voice (Rodrigues et al, 2007) prostheses. However, silicone‐based biomedical devices are associated with a likelihood of nosocomial infections as the silicone surface is prone to bacterial adhesion and biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of escherichia coli and proteus mirabilis adhesion and biofilm formation on medical grade silicone surface

Wang¹,

Neoh²,

Shi³

et al. 2011

Biotech & Bioengineering

136

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Silicone has been utilized extensively for biomedical devices due to its excellent biocompatibility and biodurability properties. However, its surface is easily colonized by bacteria which will increase the probability of nosocomial infection. In the present work, a hydrophilic antimicrobial carboxymethyl chitosan (CMCS) layer has been grafted on medical grade silicone surface pre-treated with polydopamine (PDA). The increase in hydrophilicity was confirmed from contact angle measurement. Bacterial adhesion tests showed that the PDA-CMCS coating reduced the adhesion of Escherichia coli and Proteus mirabilis by ≥ 90%. The anti-adhesion property was preserved even after the aging of the functionalized surfaces for 21 days in phosphate-buffered saline (PBS), and also after autoclaving at 121°C for 20 min. Both E. coli and P. mirabilis readily form biofilms on the pristine surface under static and flow conditions but with the PDA-CMCS layer, biofilm formation is inhibited. The flow experiments indicated that it is more difficult to inhibit biofilm formation by the highly motile P. mirabilis as compared to E. coli. No significant cytotoxicity of the modified substrates was observed with 3T3 fibroblasts.

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Candida albicans adherence on silicone elastomers: Effect of polymerisation duration and exposure to simulated saliva and nasal secretion

Cited by 20 publications

References 35 publications

Prevalence of Candida albicans and Streptococcus aureus on maxillary obturators, maxillary defects and in saliva: A cross-sectional study

Prevalence of Candida albicans and Streptococcus aureus on maxillary obturators, maxillary defects and in saliva: A cross-sectional study

Antifungal properties and biocompatibility of silver nanoparticle coatings on silicone maxillofacial prostheses in vitro

Inhibition of escherichia coli and proteus mirabilis adhesion and biofilm formation on medical grade silicone surface

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