Cancer Stem Cells: Novel Target Using Dietary Components for Prevention and Treatment

Dhar, Animesh; Fogt, Laura; Subramaniam, Dharmalingam; Anant, Shrikant

doi:10.1007/978-94-007-2630-7_2

Cited by 3 publications

(4 citation statements)

References 187 publications

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“…We have recently demonstrated that Doublecortin Calmodulin-like Kinase 1 (DCKL1) is a marker of quiescent stem cells in a variety of cancers including colon cancers [32]. Since, BMW extracts inhibited colonosphere formation, we determined whether DCLK1 expression was affected.…”

Section: Resultsmentioning

confidence: 99%

Methanolic Extracts of Bitter Melon Inhibit Colon Cancer Stem Cells by Affecting Energy Homeostasis and Autophagy

Kwatra

Subramaniam

Ramamoorthy

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Bitter melon fruit is recommended in ancient Indian and Chinese medicine for prevention/treatment of diabetes. However its effects on cancer progression are not well understood. Here, we have determined the efficacy of methanolic extracts of bitter melon on colon cancer stem and progenitor cells. Both, whole fruit (BMW) and skin (BMSk) extracts showed significant inhibition of cell proliferation and colony formation, with BMW showing greater efficacy. In addition, the cells were arrested at the S phase of cell cycle. Moreover, BMW induced the cleavage of LC3B but not caspase 3/7, suggesting that the cells were undergoing autophagy and not apoptosis. Further confirmation of autophagy was obtained when western blots showed reduced Bcl-2 and increased Beclin-1, Atg 7 and 12 upon BMW treatment. BMW reduced cellular ATP levels coupled with activation of AMP activated protein kinase; on the other hand, exogenous additions of ATP lead to revival of cell proliferation. Finally, BMW treatment results in a dose-dependent reduction in the number and size of colonospheres. The extracts also decreased the expression of DCLK1 and Lgr5, markers of quiescent, and activated stem cells. Taken together, these results suggest that the extracts of bitter melon can be an effective preventive/therapeutic agent for colon cancer.

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Section: Resultsmentioning

confidence: 99%

Methanolic Extracts of Bitter Melon Inhibit Colon Cancer Stem Cells by Affecting Energy Homeostasis and Autophagy

Kwatra

Subramaniam

Ramamoorthy

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…Spheroids were prepared as previously described [ 5 ]. Briefly, 1000 cells/well in DMEM/F12 (Invitrogen, France) supplemented with EGF (20 ng/ml, Invitrogen) and B27 supplement (1x, Invitrogen) were distributed in in ultra-low attachment 6 well plates.…”

Section: Methodsmentioning

confidence: 99%

“…Recent evidence suggests the existence of small populations of cancer initiating cells (CSC), which are believed to be responsible for tumor initiation and progression as well as resistance to chemotherapy and radiation. Pancreatic CSC can be allowed to divide and grow in ultra-low binding tissue culture dishes to form multicellular spheroids called pancospheres [ 5 , 6 ]. Recent studies have identified a rare group of label retaining cancer stem cells that have the capacity to regenerate and also divide and develop tumors.…”

Section: Introductionmentioning

confidence: 99%

Crocetinic acid inhibits hedgehog signaling to inhibit pancreatic cancer stem cells

et al. 2015

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Pancreatic cancer is the fourth leading cause of cancer deaths in the US and no significant treatment is currently available. Here, we describe the effect of crocetinic acid, which we purified from commercial saffron compound crocetin using high performance liquid chromatography. Crocetinic acid inhibits proliferation of pancreatic cancer cell lines in a dose- and time-dependent manner. In addition, it induced apoptosis. Moreover, the compound significantly inhibited epidermal growth factor receptor and Akt phosphorylation. Furthermore, crocetinic acid decreased the number and size of the pancospheres in a dose-dependent manner, and suppressed the expression of the marker protein DCLK-1 (Doublecortin Calcium/Calmodulin-Dependent Kinase-1) suggesting that crocetinic acid targets cancer stem cells (CSC). To understand the mechanism of CSC inhibition, the signaling pathways affected by purified crocetinic acid were dissected. Sonic hedgehog (Shh) upon binding to its cognate receptor patched, allows smoothened to accumulate and activate Gli transcription factor. Crocetinic acid inhibited the expression of both Shh and smoothened. Finally, these data were confirmed in vivo where the compound at a dose of 0.5 mg/Kg bw suppressed growth of tumor xenografts. Collectively, these data suggest that purified crocetinic acid inhibits pancreatic CSC, thereby inhibiting pancreatic tumorigenesis.

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“…Natural products or compounds derived from natural products have been included in the most effective cancer treatments to date. Even today, some natural compounds are known to have antioxidant, anti-proliferative, and pro-apoptotic effects in many cancers such as breast, colon, brain, melanoma, and pancreas [7].…”

Section: Introductionmentioning

confidence: 99%

Angelica sylvestris and Delphinium staphisagria Extracts Induces Antiproliferation through Caspase-mediated Apoptosis on Human Cancer Cells

Akgün

Akgun

Sahin

et al. 2022

Braz. arch. biol. technol.

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Angelica sylvestris and Delphinium staphisagria are medicinal and aromatic herbs with a long history in medicine and food industry. In this study, we have investigated anti-cancer activity of Angelica sylvestris and Delphinium staphisagria extracts on various cell lines of lung (A549), breast (MCF-7), colon (HT-29), and cervix (HeLa) origin. Also, cytotoxicity was tested on human healthy bronchial epithelial (BEAS-2B) cells. In vitro experiments showed that plant extracts suppressed cell growth and proliferation at low concentrations by reducing cell viability on cancer cells in a time and concentration-dependent manner. It was observed that Angelica sylvestris was more effective in HT-29 and HeLa cells and Delphinium staphisagria in A549 and MCF-7 cells by suppressing cell proliferation and increasing cell death. Cell death mode (apoptosis/necrosis) was investigated via fluorescent imaging, caspase-cleaved cytokeratin 18, activated caspase-3, and cleaved-PARP (poly (ADP-ribose) polymerase). In order to evaluate the cell death HIGHLIGHTS• Anticancer activities of ASE and DSE extracts were investigated.• Angelica sylvestris have anticancer potential on HT-29 and HeLa cells.• Delphinium staphisagria have anticancer potential on A549 and MCF-7 cells.• ASE and DSE extracts induce apoptosis on cancer cells.

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Cancer Stem Cells: Novel Target Using Dietary Components for Prevention and Treatment

Cited by 3 publications

References 187 publications

Methanolic Extracts of Bitter Melon Inhibit Colon Cancer Stem Cells by Affecting Energy Homeostasis and Autophagy

Methanolic Extracts of Bitter Melon Inhibit Colon Cancer Stem Cells by Affecting Energy Homeostasis and Autophagy

Crocetinic acid inhibits hedgehog signaling to inhibit pancreatic cancer stem cells

Angelica sylvestris and Delphinium staphisagria Extracts Induces Antiproliferation through Caspase-mediated Apoptosis on Human Cancer Cells

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