Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels

Zhou, Chenfei; Wei, W.; Ma, Jing; Yang, Yang; Liang, Luo-Jiao; Zhang, Yanmei; Wang, Zi-Ci; Chen, Xiaojing; Huang, Lei; Wang, Wei; Wu, Sha

doi:10.1016/j.ymthe.2020.12.034

Cited by 88 publications

(63 citation statements)

References 52 publications

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“…To test the suppressive effect of PD-L1 + EC cells on CD8 + T cells immunity in vivo, we treated the mice with a subcutaneous injection of a total of 5 × 10 6 Ishikawa cells and with intravenous injection activated CD8 + T cells (2:0 × 10 6 ) from human PBMCs [41]. For in vivo checkpoint blockade, 5-week mice were administrated with 250 μg PD1 monoclonal antibody through intraperitoneal injection (IP) three times [42].…”

Section: Reverse Transcription and Quantitative Real-timementioning

confidence: 99%

KIF2C Is a Novel Prognostic Biomarker and Correlated with Immune Infiltration in Endometrial Cancer

Zhang

Feng

et al. 2021

Stem Cells International

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Endometrial cancer (EC) is commonly diagnosed cancer in women, and the prognosis of advanced types of EC is extremely poor. Kinesin family member 2C (KIF2C) has been reported as an oncogene in cancers. However, its pathophysiological roles and the correlation with tumor-infiltrating lymphocytes in EC remain unclear. The mRNA and protein levels of KIF2C in EC tissues were detected by qRT-PCR, Western blot (WB), and IHC. CCK8, Transwell, and colony formation assay were applied to assess the effects of KIF2C on cell proliferation, migration, and invasion. Cell apoptosis and cell cycle were analyzed by flow cytometry. The antitumor effect was further validated in the nude mouse xenograft cancer model and humanized mouse model. KIF2C expression was higher in EC. Knockdown of KIF2C prolonged the G1 phases and inhibited EC cell proliferation, migration, and invasion in vitro. Bioinformatics analysis indicated that KIF2C is negatively correlated with the infiltration level of CD8+ T cells but positively with the poor prognosis of EC patients. The apoptosis of CD8+ T cell was inhibited after the knockdown of KIF2C and was further inhibited when it is combined with anti-PD1. Conversely, compared to the knockdown of KIF2C expression alone, the combination of anti-PD1 further promoted the apoptosis of Ishikawa and RL95-2 cells. Moreover, the knockdown of KIF2C inhibited the expression of Ki-67 and the growth of tumors in the nude mouse xenograft cancer model. Our study found that the antitumor efficacy was further evaluated by the combination of anti-PD1 and KIF2C knockdown in a humanized mouse model. This study indicated that KIF2C is a novel prognostic biomarker that determines cancer progression and also a target for the therapy of EC and correlated with tumor immune cells infiltration in EC.

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Section: Reverse Transcription and Quantitative Real-timementioning

confidence: 99%

KIF2C Is a Novel Prognostic Biomarker and Correlated with Immune Infiltration in Endometrial Cancer

Zhang

Feng

et al. 2021

Stem Cells International

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“…This positive feedback through STAT3 hyperactivation could be critical for cell cycle progression and neoplastic transformation [ 59 ]. Another miRNA, the exosomal miR-1468-5p, can suppress HMBOX1-SOCS1 expression and activate JAK2/STAT3 signaling in lymphocytes promoting immune escape of cancer cells and tumor progression [ 60 ].…”

Section: Rna In Extracellular Vesiclesmentioning

confidence: 99%

“…Small arrows indicate increase or decrease of the molecules or processes indicated. References for a: [ 24 , 42 ]; b: [ 25 ]; c: [ 26 ]; d: [ 33 ]; e: [ 55 , 56 , 57 , 64 ]; f: [ 58 ]; g: [ 59 , 60 ]. Some images are from .…”

Section: Rna In Extracellular Vesiclesmentioning

confidence: 99%

Extracellular Vesicles in Cervical Cancer and HPV Infection

et al. 2021

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Since their description, extracellular vesicles (EVs) have shown growing relevance in cancer progression. These cell structures contain and transfer molecules such as nucleic acids (including DNA and RNA), proteins, and lipids. Despite the rising information about EVs’ relationship with cancer, there is still scarce evidence about their content and function in cervical cancer. Interestingly, the composition and purposes of some cellular molecules and the expression of oncogenic proteins packaged in EVs seem modified in HPV-infected cells; and, although only the E6 oncogenic protein has been detected in exosomes from HPV-positive cells, both E6/E7 oncogenes mRNA has been identified in EVs; however, their role still needs to be clarified. Given that EVs internalizing into adjacent or distant cells could modify their cellular behavior or promote cancer-associated events like apoptosis, proliferation, migration, or angiogenesis in receptor cells, their comprehensive study will reveal EV-associated mechanisms in cervical cancer. This review summarizes the current knowledge in composition and functions of cervical cancer and HPV Infection-derived EVs.

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“…30 Thus, salivary exosomes create favorable conditions for tumor migration and invasion. 31,32 At present, salivary exosomes can also assist in the delivery of anti-tumor drugs or interfere with tumor growth to exert their an anti-tumor role. 33…”

Section: Function Of Salivary Exosomesmentioning

confidence: 99%

The Role and Application of Salivary Exosomes in Malignant Neoplasms

Deng¹,

Cao²,

Wang³

et al. 2021

CMAR

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The study of salivary exosomes in malignant neoplasms has attracted widespread attention in the clinical setting. Although a variety of diagnostic and treatment approaches have been proposed, there are some limitations to their application. In recent years, the role of salivary exosomes in cancer has been increasingly studied. Salivary exosomes not only renew and regulate the biological behavior of tumor cells, such as malignant proliferation, migration, and invasion, but they also serve as ideal markers for early diagnosis of diseases and may represent an effective therapeutic target. This article reviews the current research on salivary exosomes in malignant neoplasms.

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Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels

Cited by 88 publications

References 52 publications

KIF2C Is a Novel Prognostic Biomarker and Correlated with Immune Infiltration in Endometrial Cancer

KIF2C Is a Novel Prognostic Biomarker and Correlated with Immune Infiltration in Endometrial Cancer

Extracellular Vesicles in Cervical Cancer and HPV Infection

The Role and Application of Salivary Exosomes in Malignant Neoplasms

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