Cancer risk in space due to radiation assessed by determining cell lethality and mutation frequencies of prokaryotes and a plasmid during the Second International Microgravity Laboratory (IML-2) Space Shuttle experiment

Harada, Karen Mine; Obiya, Yurino; Nakano, Tamotsu; Kawashima, Masahiro; Miki, Tetsuro; Kobayashi, Yuko; Watanabe, Hiroko; Okaichi, Kumio; Ohnishi, Takeo; Mukai, Chiaki; Nagaoka, Shunji

doi:10.3892/or.4.4.691

Cited by 8 publications

(12 citation statements)

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“…Similarly, we found that KY385 ( trpE9777 − , recA56 − ) cells were sensitive to UV radiation, and KY386 ( trpE9777 − , uvrA6 − ) cells were hypermutable when exposed to UV irradiation [22]. Furthermore, the space experimental results for derivatives of the KMBL3835 strain were similar to those for derivatives of the H/r30R strain in this flight (unpublished data) and the Second International Microgravity Laboratory (IML‐2) flight [17].…”

Section: Resultssupporting

confidence: 64%

“…We could not observe any effect of high‐LET cosmic radiation in this species [12]. No increase of supF mutation frequency of pZ189 DNA in space samples was measured as compared with ground samples in this flight (unpublished data) and the IML‐2 flight [17].…”

Section: Resultsmentioning

confidence: 85%

“…Due to the specific characteristics of the equipment on the space shuttle we must use dry cells. Dry cells of the 11 E. coli strains were prepared as follows [17]. Cells were cultivated at 37°C in a broth medium [18] containing 1.0% w/v Bacto beef extract (Difco), 1.0% w/v polypeptone and 0.2% w/v NaCl, adjusted to pH 7.4.…”

Section: Methodsmentioning

confidence: 99%

“…Deinococcus radiodurans cells (wild‐type strain MR 1 ) and shuttle vector plasmid pZ189 DNA were prepared as described previously [12, 17].…”

Section: Methodsmentioning

confidence: 99%

“…The survival criterion of D. radiodurans and the method of analysis of shuttle vector plasmid pZ189 DNA were described previously [12, 17].…”

Section: Methodsmentioning

confidence: 99%

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Lethality of high linear energy transfer cosmic radiation toEscherichia coliDNA repair-deficient mutants during the âSL-J/FMPTâ space experiment

Harada¹,

Nagaoka²,

Mohri³

et al. 1998

FEMS Microbiology Letters

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We investigated the lethal and mutagenic effects of high linear energy transfer cosmic radiation on 11 strains of Escherichia coli, including DNA repair-deficient mutants, using the Radiation Monitoring Container and Dosimeter in the space shuttle 'Endeavour' as part of the 'SL-J/FMPT' space experiment, the 'Fuwatto '92' project. After the return to earth of the shuttle, we evaluated survival and mutations of samples in space and matched controls. The surviving fractions were determined by means of colony count on broth agar plates, and the mutation frequencies were estimated by appearance of arg' revertants on minimal agar plates. The average of the total equivalent dose rate during this space flight was 0.202 mSv/day as measured by the plastic radiation detectors and the thermoluminescent dosimeters in the Radiation Monitoring Container and Dosimeter. The combined action of DNA polymerase and 3'-->5' exonuclease activities was found to make the greatest contribution to the repair of cosmic radiation-induced DNA damage, 5'-->3' exonuclease and recombination repair enzyme activities made a moderate contribution, whereas UV endonuclease activity was not involved in this DNA repair process.

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Section: Resultssupporting

confidence: 64%

Section: Resultsmentioning

confidence: 85%

Section: Methodsmentioning

confidence: 99%

“…Deinococcus radiodurans cells (wild‐type strain MR 1 ) and shuttle vector plasmid pZ189 DNA were prepared as described previously [12, 17].…”

Section: Methodsmentioning

confidence: 99%

“…The survival criterion of D. radiodurans and the method of analysis of shuttle vector plasmid pZ189 DNA were described previously [12, 17].…”

Section: Methodsmentioning

confidence: 99%

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Lethality of high linear energy transfer cosmic radiation toEscherichia coliDNA repair-deficient mutants during the âSL-J/FMPTâ space experiment

Harada¹,

Nagaoka²,

Mohri³

et al. 1998

FEMS Microbiology Letters

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Space Radiobiology

Hellweg,

Arena,

Baatout

et al. 2023

Radiobiology Textbook

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The study of the biologic effects of space radiation is considered a “hot topic,” with increased interest in the past years. In this chapter, the unique characteristics of the space radiation environment will be covered, from their history, characterization, and biological effects to the research that has been and is being conducted in the field.After a short introduction, you will learn the origin and characterization of the different types of space radiation and the use of mathematical models for the prediction of the radiation doses during different mission scenarios and estimate the biological risks due to this exposure. Following this, the acute, chronic, and late effects of radiation exposure in the human body are discussed before going into the detailed biomolecular changes affecting cells and tissues, and in which ways they differ from other types of radiation exposure.The next sections of this chapter are dedicated to the vast research that has been developed through the years concerning space radiation biology, from small animals to plant models and 3D cell cultures, the use of extremophiles in the study of radiation resistance mechanisms to the importance of ground-based irradiation facilities to simulate and study the space environment.

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Does reduced gravity alter cellular response to ionizing radiation?

Manti

2006

Radiat Environ Biophys

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This review addresses the purported interplay between actual or simulated weightlessness and cellular response to ionizing radiation. Although weightlessness is known to alter several cellular functions and to affect signaling pathways implicated in cell proliferation, differentiation and death, its influence on cellular radiosensitivity has so far proven elusive. Renewed controversy as to whether reduced gravity enhances long-term radiation risk is fueled by recently published data that claim either overall enhancement of genomic damage or no increase of radiation-induced clastogenicity by modeled microgravity in irradiated human cells. In elucidating this crucial aspect of space radiation protection, ground-based experiments, such as those based on rotating-wall bioreactors, will increasingly be used and represent a more reproducible alternative to in-flight experiments. These low-shear vessels also make three-dimensional cellular co-cultures possible and thus allow to study the gravisensitivity of radioresponse in a context that better mimics cell-to-cell communication and hence in vivo cellular behavior.

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Cancer risk in space due to radiation assessed by determining cell lethality and mutation frequencies of prokaryotes and a plasmid during the Second International Microgravity Laboratory (IML-2) Space Shuttle experiment

Cited by 8 publications

References 0 publications

Lethality of high linear energy transfer cosmic radiation toEscherichia coliDNA repair-deficient mutants during the âSL-J/FMPTâ space experiment

Lethality of high linear energy transfer cosmic radiation toEscherichia coliDNA repair-deficient mutants during the âSL-J/FMPTâ space experiment

Space Radiobiology

Does reduced gravity alter cellular response to ionizing radiation?

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Cancer risk in space due to radiation assessed by determining cell lethality and mutation frequencies of prokaryotes and a plasmid during the Second International Microgravity Laboratory (IML-2) Space Shuttle experiment

Cited by 8 publications

References 0 publications

Lethality of high linear energy transfer cosmic radiation toEscherichia coliDNA repair-deficient mutants during the âSL-J/FMPTâ space experiment

Lethality of high linear energy transfer cosmic radiation toEscherichia coliDNA repair-deficient mutants during the âSL-J/FMPTâ space experiment

Space Radiobiology

Does reduced gravity alter cellular response to ionizing radiation?

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Lethality of high linear energy transfer cosmic radiation toEscherichia coliDNA repair-deficient mutants during the âSL-J/FMPTâ space experiment

Lethality of high linear energy transfer cosmic radiation toEscherichia coliDNA repair-deficient mutants during the âSL-J/FMPTâ space experiment