Cancer‐related genetic variants of <i>Helicobacter pylori</i> strains determined using gastric wash‐based whole‐genome analysis with single‐molecule real‐time technology

Watanabe, Yoshiyuki; Oikawa, Ritsuko; Kodaka, Yasuhiro; Sato, Yoshinori; Ono, Shoko; Kenmochi, Takeshi; Suzuki, Hideo; Futagami, Seiji; Kato, Mototsugu; Yamamoto, Hiroyuki; Itoh, Fumio

doi:10.1002/ijc.33257

Cited by 8 publications

(9 citation statements)

References 27 publications

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“…Recently, GC-related genetic variants of H. pylori strains were determined using GW-based whole genome analysis with a single-molecule real-time technology. These results suggest that the hopL variant is related with the GC development and could be a genetic biomarker of H. pylori virulence and risk of GC [115].…”

Section: Gastric Wash or Gastric Juice Exosomal Dna-based Methylation Analysis Of Barhl2mentioning

confidence: 74%

Non-Invasive Early Molecular Detection of Gastric Cancers

Yamamoto

Watanabe

Sato

et al. 2020

Cancers

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Gastric cancer (GC) is a significant source of global cancer death with a high mortality rate, because the majority of patients with GC are diagnosed at a late stage, with limited therapeutic choices and poor outcomes. Therefore, development of minimally invasive or noninvasive biomarkers which are specific to GC is crucially needed. The latest advancements in the understanding of GC molecular landscapes and molecular biological methods have accelerated attempts to diagnose GC at an early stage. Body fluids, including peripheral blood, saliva, gastric juice/wash, urine, and others, can be a source of biomarkers, offering new methods for the early detection of GC. Liquid biopsy-based methods using circulating sources of cancer nucleic acids could also be considered as alternative strategies. Moreover, investigating gastric juices/washes could represent an alternative for the detection of GC via invasive biopsy. This review summarizes recently reported biomarkers based on DNA methylation, microRNA, long noncoding RNA, circular RNA, or extracellular vesicles (exosomes) for the detection of GC. Although the majority of studies have been conducted to detect these alterations in advanced-stage GC and only a few in population studies or early-stage GC, some biomarkers are potentially valuable for the development of novel approaches for an early noninvasive detection of GC.

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Section: Gastric Wash or Gastric Juice Exosomal Dna-based Methylation Analysis Of Barhl2mentioning

confidence: 74%

Non-Invasive Early Molecular Detection of Gastric Cancers

Yamamoto

Watanabe

Sato

et al. 2020

Cancers

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“…Mechanisms of malignant transformation due to genetic abnormalities include driver gene mutations and the accumulation of passenger gene abnormalities due to epigenetic alterations including DNA and microRNA methylation 24–26 . In gastric cancer, the accumulation of abnormalities due to epigenetic gene alterations from Helicobacter pylori infection is important in tumorigenesis 27–32 . Multiple studies have investigated the clinical applications of these candidate molecular markers for diagnosis; while the “risk and predictive diagnoses” for malignant gastric transformation has been achieved, its practical applications to diagnose the presence, site, and extent of lesions have not been sufficiently realized.…”

Section: Discussionmentioning

confidence: 99%

“… 24 , 25 , 26 In gastric cancer, the accumulation of abnormalities due to epigenetic gene alterations from Helicobacter pylori infection is important in tumorigenesis. 27 , 28 , 29 , 30 , 31 , 32 Multiple studies have investigated the clinical applications of these candidate molecular markers for diagnosis; while the “risk and predictive diagnoses” for malignant gastric transformation has been achieved, its practical applications to diagnose the presence, site, and extent of lesions have not been sufficiently realized.…”

Section: Discussionmentioning

confidence: 99%

Combination of artificial intelligence‐based endoscopy and miR148a methylation for gastric indefinite dysplasia diagnosis

Watanabe

Oikawa

Agawa

et al. 2021

Clinical Laboratory Analysis

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Background and Aim Gastrointestinal endoscopy and biopsy‐based pathological findings are needed to diagnose early gastric cancer. However, the information of biopsy specimen is limited because of the topical procedure; therefore, pathology doctors sometimes diagnose as gastric indefinite for dysplasia (GIN). Methods We compared the accuracy of physician‐performed endoscopy (trainee, n = 3; specialists, n = 3), artificial intelligence (AI)‐based endoscopy, and/or molecular markers (DNA methylation: BARHL2, MINT31, TET1, miR‐148a, miR‐124a‐3, NKX6‐1; mutations: TP53; and microsatellite instability) in diagnosing GIN lesions. We enrolled 24,388 patients who underwent endoscopy, and 71 patients were diagnosed with GIN lesions. Thirty‐two cases of endoscopic submucosal dissection (ESD) in 71 GIN lesions and 32 endoscopically resected tissues were assessed by endoscopists, AI, and molecular markers to identify benign or malignant lesions. Results The board‐certified endoscopic physicians group showed the highest accuracy in the receiver operative characteristic curve (area under the curve [AUC]: 0.931), followed by a combination of AI and miR148a DNA methylation (AUC: 0.825), and finally trainee endoscopists (AUC: 0.588). Conclusion AI with miR148s DNA methylation‐based diagnosis is a potential modality for diagnosing GIN.

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“…Among the carcinogenic factors, there are many physical and chemical factors, such as ultraviolet rays ( Rivas et al., 2020 ) and nitrosamines ( Liu et al., 2021 ). Another factor is the biological factor that causes cancer, that is, infection caused by microorganisms, viruses and bacteria ( De Martel et al., 2017 ; Watanabe et al., 2021 ). Using metagenome sequencing, 16S rRNA detection and other technologies, the microbial community composition of various cancers, such as colon cancer, pancreatic cancer, breast cancer, and lung cancer, was studied and identified, and the existence of bacteria, viruses and other microorganisms was found ( Nejman et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Bacterial DNA involvement in carcinogenesis

Yangyanqiu

Shuwen²

2022

Front. Cell. Infect. Microbiol.

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The incidence of cancer is high worldwide, and biological factors such as viruses and bacteria play an important role in the occurrence of cancer. Helicobacter pylori, human papillomavirus, hepatitis B viruses and other organisms have been identified as carcinogens. Cancer is a disease driven by the accumulation of genome changes. Viruses can directly cause cancer by changing the genetic composition of the human body, such as cervical cancer caused by human papillomavirus DNA integration and liver cancer caused by hepatitis B virus DNA integration. Recently, bacterial DNA has been found around cancers such as pancreatic cancer, breast cancer and colorectal cancer, and the idea that bacterial genes can also be integrated into the human genome has become a hot topic. In the present paper, we reviewed the latest phenomenon and specific integration mechanism of bacterial DNA into the human genome. Based on these findings, we also suggest three sources of bacterial DNA in cancers: bacterial DNA around human tissues, free bacterial DNA in bacteremia or sepsis, and endogenous bacterial DNA in the human genome. Clarifying the theory that bacterial DNA integrates into the human genome can provide a new perspective for cancer prevention and treatment.

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Cancer‐related genetic variants of Helicobacter pylori strains determined using gastric wash‐based whole‐genome analysis with single‐molecule real‐time technology

Cited by 8 publications

References 27 publications

Non-Invasive Early Molecular Detection of Gastric Cancers

Non-Invasive Early Molecular Detection of Gastric Cancers

Combination of artificial intelligence‐based endoscopy and miR148a methylation for gastric indefinite dysplasia diagnosis

Bacterial DNA involvement in carcinogenesis

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