Cancer protein biomarker identification and quantification using nanoforest substrate and hand-held Raman spectrometer

Kim, Hyun Soo; Lee, Taeha; Yun, Jongsu; Lee, Gyudo; Hong, Yoochan

doi:10.1016/j.microc.2020.105632

Cited by 7 publications

(10 citation statements)

References 25 publications

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“…Prior studies have established that Raman signals can be strengthened by distributing noble metal nanostructures in close proximity. 21,23 Accordingly, in this study, the SERS effect was optimized by varying the mole numbers of Ag for SNSF on the AKD-treated hydrophobic filter paper (Figure 3d). As depicted in Figure 3e Moreover, when comparing the potential of SNSF and SNP (Figure 4c), all signal measurements were performed considering the abovementioned Ag mole number (62.38 μmol).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Moreover, the droplet was observed to remain on the surface of the hydrophobic filter paper for up to an hour. Prior studies have established that Raman signals can be strengthened by distributing noble metal nanostructures in close proximity. , Accordingly, in this study, the SERS effect was optimized by varying the mole numbers of Ag for SNSF on the AKD-treated hydrophobic filter paper (Figure d). As depicted in Figure e, the SNSF distributions in the filter paper-based SERS substrates with varying Ag mole numbers were confirmed via field-emission scanning electron microscopy.…”

Section: Resultsmentioning

confidence: 99%

“…Raman Spectra Measurement. The Raman spectra were acquired in accordance to a previously published report 21 with modifications. They were collected using 785 nm laser excitation.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…12 Moreover, the associated instrumentation can be miniaturized to the hand-held scale. 13 Furthermore, the Raman spectra provide highly specific chemical structure fingerprints. The typically weak signals obtained via Raman spectroscopy can be strengthened via surface-enhanced Raman scattering (SERS) that capitalizes on the intrinsic properties of plasmonic metals.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Raman spectroscopy-based analysis can overcome the drawbacks of the aforementioned analytical approaches because it can rapidly analyze samples in any physical state without destruction . Moreover, the associated instrumentation can be miniaturized to the hand-held scale . Furthermore, the Raman spectra provide highly specific chemical structure fingerprints.…”

Section: Introductionmentioning

confidence: 99%

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Hand-Held Raman Spectrometer-Based Dual Detection of Creatinine and Cortisol in Human Sweat Using Silver Nanoflakes

Kim

Lee

et al. 2021

Anal. Chem.

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The conventional tissue biopsy method yields isolated snapshots of a narrow region. Therefore, it cannot facilitate comprehensive disease characterization and monitoring. Recently, the detection of tumor-derived components in body fluidsa practice known as liquid biopsyhas attracted increased attention from the biochemical research and clinical application viewpoints. In this vein, surface-enhanced Raman scattering (SERS) has been identified as one of the most powerful liquidbiopsy analysis techniques, owing to its high sensitivity and specificity. Moreover, it affords high-capacity spectral multiplexing for simultaneous target detection and a unique ability to obtain intrinsic biomolecule-fingerprint spectra. This paper presents the fabrication of silver nanosnowflakes (SNSFs) using the polyol method and their subsequent dropping onto a hydrophobic filter paper. The SERS substrate, which comprises the SNSFs and hydrophobic filter paper, facilitates the simultaneous detection of creatinine and cortisol in human sweat using a hand-held Raman spectrometer. The proposed SERS system affords Raman spectrometry to be performed on small sample volumes (2 μL) to identify the normal and at-risk creatinine and cortisol groups.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…Raman Spectra Measurement. The Raman spectra were acquired in accordance to a previously published report 21 with modifications. They were collected using 785 nm laser excitation.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hand-Held Raman Spectrometer-Based Dual Detection of Creatinine and Cortisol in Human Sweat Using Silver Nanoflakes

Kim

Lee

et al. 2021

Anal. Chem.

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Biomarkers for Cancer Drug Development

Rehman

2022

Cancer Biomarkers in Diagnosis and Therapeutics

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On-bead DNA synthesis triggered by allosteric probe for detection of carcinoembryonic antigen

et al. 2022

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Sensitive quantification of protein biomarkers is highly desired for clinical diagnosis and treatment. Yet, unlike DNA/RNA which can be greatly amplified by PCR/RT-PCR, the amplification and detection of trace amount of proteins remain a great challenge. Here, we combined allosteric probe (AP) with magnetic bead (MB) for assembling an on-bead DNA synthesis system (named as APMB) to amplify protein signals. The AP is designed and conjugated onto the MB, enabling the protein biomarker to be separated and enriched. Once recognizing the biomarker, the AP alters its conformation to initiate DNA synthesis on beads for primary signal amplification. During the DNA synthesis, biotin-dATPs are incorporated into the newly synthesized DNA strands. Then, the biotin-labeled DNA specifically captures streptavidin (STR)–conjugated horseradish peroxidase (HRP), which is used to catalyze a colorimetric reaction for secondary signal amplification. By using carcinoembryonic antigen (CEA) as a protein model, the APMB can quantify protein biomarkers of as low as 0.01 ng/mL. The response values measured by APMB are linearly related to the protein concentrations in the range 0.05 to 20 ng/mL. Clinical examination demonstrated good practicability of the APMB in quantifying serum protein biomarker. The on-bead DNA synthesis could be exploited to improve protein signal amplification, thus facilitating protein biomarker detection of low abundance for early diagnosis. Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-022-05404-4.

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Cancer protein biomarker identification and quantification using nanoforest substrate and hand-held Raman spectrometer

Cited by 7 publications

References 25 publications

Hand-Held Raman Spectrometer-Based Dual Detection of Creatinine and Cortisol in Human Sweat Using Silver Nanoflakes

Hand-Held Raman Spectrometer-Based Dual Detection of Creatinine and Cortisol in Human Sweat Using Silver Nanoflakes

Biomarkers for Cancer Drug Development

On-bead DNA synthesis triggered by allosteric probe for detection of carcinoembryonic antigen

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