Cancer gene therapy

Mitrović, Tatjana; Radulović, Siniša

doi:10.2298/aoo0501023m

Cited by 3 publications

(2 citation statements)

References 39 publications

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“…Cancer gene therapy can be defined as the use of genetic material to manipulate tumor or normal cells to encourage anti-tumor activity such as direct killing of cells, immunomodulation or correction of genetic errors, and reversion of malignant status [ 14 ]. A successful cancer gene therapy requires an appropriate gene which displays selective toxicity toward tumor cells without eliciting harmful effects in normal cells or tissues.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of autophagy by 3-MA enhances IL-24-induced apoptosis in human oral squamous cell carcinoma cells

Yang

Wang

et al. 2015

J Exp Clin Cancer Res

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BackgroundInterleukin-24(IL-24), also referred to as melanoma differentiation-associated gene-7(mda-7), is a unique member of the IL-10 gene family, which displays nearly ubiquitous cancer-specific toxicity. The most notable feature of IL-24 is selectively induced growth suppression and apoptosis in various cancer cells, with no harmful effects toward normal cells. Autophagy is a self-protective mechanism in many kinds of tumor cells that respond to anticancer treatment. It is reported that autophagy inhibition could enhance the effects of many kinds of anticancer treatments, including gene therapy. However, whether IL-24 is effective to treat oral squamous cell carcinomas (OSCC) and if autophagy inhibition could improve the anticancer effect of IL-24 towards OSCC is has not been detected.MethodsMTT assays were carried out to determine the cell proliferation; Transfection was used to gene transfer; Western Blot was performed to detect the protein level of LC3II, P62, Beclin 1, Cleaved caspase-3, β-Tubulin and β-actin; Apoptosis rates and cell cycle alteration were analyzed using flow cytometry; Autophagy induction was confirmed by MDC staining, GFP-LC3 staining and transmission electron microscopy. Amount of IL-24 in the culture medium was quantified by ELISA. Apoptosis in vivo was analyzed by TUNEL assay. HE staining was used to observe the morphology of the samples.ResultsIn the present study, we proved that IL-24 have a novel anticancer effect towards KB cells and that autophagy inhibition could improve the anticancer effect of IL-24. IL-24 treated cells showed autophagy characteristics and autophagy inhibition by 3-methyladenine (3-MA) significantly enhanced IL-24-induced apoptosis. Similar results were obtained in the KB cells xenograft tumor model.ConclusionsThese results suggest that the combination of autophagy inhibitors and IL-24 based on the AdLTR2EF1α-mediated gene transfer could be a promising way to cure OSCC.

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Section: Discussionmentioning

confidence: 99%

Inhibition of autophagy by 3-MA enhances IL-24-induced apoptosis in human oral squamous cell carcinoma cells

Yang

Wang

et al. 2015

J Exp Clin Cancer Res

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“…The objective of gene therapy is to introduce new genetic material into target cells while causing no harm to the surrounding normal tissue. There are two main approaches: In vivo gene transfer where ingenes are delivered directly to target cells in the body and Ex vivo gene transfer wherein target cells are genetically modified outside the body and then reimplanted [12] .The transfer of genetic material into the target cells is brought about mainly by viral and the nonviral vectors. The viral vectors include a host of viruses such as retrovirus, adenovirus, adeno -associated virus, herpes virus and lentivirus.…”

Section: Gene Transfer Concept and Deliv-ery Systemmentioning

confidence: 99%

Gene Therapy in Head and Neck Cancer

Gaonkar¹,

Patankar²,

Bhandare³

et al. 2016

Journal of Tumor

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Advancements in the field of molecular biology have stimulated the interest of the scientists and clincians worldwide who believe that genetic manipulation can be a potential cure for cancer. Gene therapy is one such therapy that is significantly applied in human cancer which involves transferring genetic material into a host cell via a viral or non-viral vector, immunotherapy or by manipulation of the tumor microenvironment to reduce angiogenesis.It is not only being considered as a therapeutic strategy to treat cancer but also as a medium to counter the post radiation side-effects. More recently it has been used as a means to prevent the progression of potentially malignant diseases into cancer thereby proving to be a potential candidate for improving survival rates in cancer patients. This review aims to provide insight into the field of cancer gene therapy as applicable to oral squamous cell carcinoma and oral premalignancies that may have far reaching effects on the way a cancer patient is managed.

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Degradable Polyethylenimine-Based Gene Carriers for Cancer Therapy

et al. 2017

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Gene therapy using recombinant DNA or gene silencing using siRNA have become a prominent area of research in cancer therapy. However, their use in clinical applications is limited due to overall safety concerns and suboptimal efficacy. Although non-viral vectors such as polycationic polymers do not offer the same level of transfection efficiency as their viral counterparts, they still demonstrate immense potential as alternatives to viral vectors, given their versatility, low immunogenicity, ease of large-scale production, and ability to accelerate gene transfer with well-designed delivery platforms. Among these polymers, polyethylenimine (PEI) is considered a state-of-the-art gene carrier, owing to its ability to improve gene transfer capacity and intracellular delivery. Nonetheless, PEI suffers from the critical shortcoming of non-degradability that can lead to severe cytotoxic effects, despite the fact that the level of this toxicity decreases with molecular weight (MW). As a result, a considerable amount of effort has been devoted to designing low-MW PEI derivatives with degradable linkages. This review will categorize the recent advances in these degradable PEI derivatives based on their degradable chemistries, including ester, disulfide, imine, carbamate, amide, and ketal linkages, and summarize their application in gene therapies against various major cancer malignancies.

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Cancer gene therapy

Cited by 3 publications

References 39 publications

Inhibition of autophagy by 3-MA enhances IL-24-induced apoptosis in human oral squamous cell carcinoma cells

Inhibition of autophagy by 3-MA enhances IL-24-induced apoptosis in human oral squamous cell carcinoma cells

Gene Therapy in Head and Neck Cancer

Degradable Polyethylenimine-Based Gene Carriers for Cancer Therapy

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