Cancer Family History Questionnaires Telephone Survey

Appleby-Tagoe, Julia H.; Foulkes, William D.; Palma, Laura

doi:10.1037/t40898-000

Cited by 7 publications

(8 citation statements)

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“…16,17,36 This functionality should complement other reported methods for protein N-terminal modification, such as expressed protein ligation, transpeptidase-based ligation strategies, artificially split inteins and various protein chemistry methods. [36][37][38][39][40][41] Furthermore, under specific contexts, the extein preferences observed will favor the application of Cat in protein engineering: Cat exhibits promiscuous activity in the presence of substitutions within the C-extein but is highly vulnerable to mutations within the N-extein. This preference is complementary to the commonly applied naturally split DnaE inteins, which are sensitive to C-extein mutations.…”

Section: Discussionmentioning

confidence: 99%

An Atypical Mechanism of Split Intein Molecular Recognition and Folding

et al. 2018

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Split inteins associate to trigger protein splicing in trans, a post-translational modification in which protein sequences fused to the intein pair are ligated together in a traceless manner. Recently, a family of naturally split inteins has been identified that is split at a noncanonical location in the primary sequence. These atypically split inteins show considerable promise in protein engineering applications; however, the mechanism by which they associate is unclear and must be different from that of previously characterized canonically split inteins due to unique topological restrictions. Here, we use a consensus design strategy to generate an atypical split intein pair (Cat) that has greatly improved activity and is amenable to detailed biochemical and biophysical analysis. Guided by the solution structure of Cat, we show that the association of the fragments involves a disorder-to-order structural transition driven by hydrophobic interactions. This molecular recognition mechanism satisfies the topological constraints of the intein fold and, importantly, ensures that premature chemistry does not occur prior to fragment complementation. Our data lead a common blueprint for split intein complementation in which localized structural rearrangements are used to drive folding and regulate protein-splicing activity.

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Section: Discussionmentioning

confidence: 99%

An Atypical Mechanism of Split Intein Molecular Recognition and Folding

et al. 2018

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“…Carbon monoxide is produced as a byproduct in the steam reforming of hydrocarbons, autothermal reforming, and partial natural gas oxidation. It is possible to remove most of the carbon monoxide by the water-gas shift (WGS) reaction, , which can reduce carbon monoxide to 1% of the feed but not eliminate it completely. The preferential oxidation (PROX) reaction is a possible subsequent step to further decrease the carbon monoxide concentration to acceptable concentrations, typically below 10 ppm.…”

Section: Introductionmentioning

confidence: 99%

“…It is possible to remove most of the carbon monoxide by the water-gas shift (WGS) reaction, , which can reduce carbon monoxide to 1% of the feed but not eliminate it completely. The preferential oxidation (PROX) reaction is a possible subsequent step to further decrease the carbon monoxide concentration to acceptable concentrations, typically below 10 ppm. A disadvantage of the PROX reaction is the possible loss of hydrogen due to its oxidation, so that special reactor designs and temperature regimes, in addition to catalyst development, are required to maximize selective carbon monoxide oxidation.…”

Section: Introductionmentioning

confidence: 99%

Evolution of the Atomic Structure of Ceria-Supported Platinum Nanocatalysts: Formation of Single Layer Platinum Oxide and Pt–O–Ce and Pt–Ce Linkages

et al. 2016

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Depending on platinum loading and gas environment vastly different platinum structures in Pt/ceria were observed by Pt L3-edge extended X-ray absorption (EXAFS) and X-ray absorption near-edge (XANES) spectroscopies. Calcination of highly loaded platinum (4 wt %) yielded stacked layers of α-PtO2. The low platinum-loaded sample (2 wt %), obtained by leaching, consisted of a single layer of α-PtO2 bonded to the support by Pt–O–Ce linkages. Reduction of the platinum oxide by exposure to a carbon monoxide and hydrogen (PROX) gas mixture led to complete metal reduction at temperatures above 540 K. Under these conditions platinum–cerium bonds with a distance of 2.4 Å were detected and particles of 0.8 and 0.6 nm for the high-loaded and low-loaded Pt/ceria samples, respectively.

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“…The efficiency of the oxygen reduction reaction (ORR), 4H + + 4e– + O 2 → 2H 2 O, at the cathode of a polymer electrolyte membrane fuel cell (PEMFC) is a critical issue for commercial application of this type of fuel cells. − The best current catalysts are Pt and Pt-based binary alloys, such as Pt 3 Ni. , The origin of the superior performance of the Pt 3 Ni alloy has not been clearly understood yet. Some researchers believe it is due to the shift of the d-band center to the desired region that occurs due to alloying Pt with Ni. , Others came to the conclusion that alloying makes OH ad removal favorable, increasing the surface area available for O 2 binding .…”

Section: Introductionmentioning

confidence: 99%

Mechanism for Oxygen Reduction Reaction on Pt₃Ni Alloy Fuel Cell Cathode

Sha

Merinov

et al. 2012

J. Phys. Chem. C

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We use quantum mechanics, density functional theory at the PBE level, to predict the binding-site preferences and reaction barriers for all intermediates involved in the oxygen reduction reaction (ORR) on the low energy surface of Pt 3 Ni alloy. Here we calculate that the surface layer is Ni depleted (100% Pt) while the second layer is Ni enriched (50% Pt) as shown by experiment. Even though the top layer is pure Pt, we find that the sublayer Ni imposes strong preferences in binding sites for most intermediates, which in turn strongly influences the reaction barriers. This strong preference leads to a strong site dependence of the barriers. Considering water as the solvent, we predict that, at low coverage of O ad and OH ad , the barrier for the rate-determining step is 0.81 eV, whereas, at high coverage, this barrier decreases to 0.43 eV. It can be compared to a barrier of 0.50 eV for pure Pt, explaining the improved ORR rate for the Pt 3 Ni alloy. We report the results both for gas phase and for aqueous phase environments.

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Cancer Family History Questionnaires Telephone Survey

Cited by 7 publications

References 0 publications

An Atypical Mechanism of Split Intein Molecular Recognition and Folding

An Atypical Mechanism of Split Intein Molecular Recognition and Folding

Evolution of the Atomic Structure of Ceria-Supported Platinum Nanocatalysts: Formation of Single Layer Platinum Oxide and Pt–O–Ce and Pt–Ce Linkages

Mechanism for Oxygen Reduction Reaction on Pt₃Ni Alloy Fuel Cell Cathode

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Cancer Family History Questionnaires Telephone Survey

Cited by 7 publications

References 0 publications

An Atypical Mechanism of Split Intein Molecular Recognition and Folding

An Atypical Mechanism of Split Intein Molecular Recognition and Folding

Evolution of the Atomic Structure of Ceria-Supported Platinum Nanocatalysts: Formation of Single Layer Platinum Oxide and Pt–O–Ce and Pt–Ce Linkages

Mechanism for Oxygen Reduction Reaction on Pt3Ni Alloy Fuel Cell Cathode

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Mechanism for Oxygen Reduction Reaction on Pt₃Ni Alloy Fuel Cell Cathode