Cáncer de colon experimental: caracterización del modelo mediante marcadores tumorales y moléculas de adhesión

Carmona, Fernando Roux

doi:10.14201/gredos.76575

Cited by 2 publications

(2 citation statements)

References 156 publications

(207 reference statements)

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“…Chemical induction has also been accepted as an appropriate tool for tumour induction. Various compounds, including dimethylhydrazine and its metabolites methylnitronitrosoguanidine, N-Nitroso-N-methylurea and azoxymethane, are able to induce tumour and/or metastases growth in laboratory animals 25 – 29 . These compounds have alkylating activity, which cause breakage of DNA chains, abnormal pairing of bases, and inhibition of cell division, finally, resulting in cell death.…”

Section: Discussionmentioning

confidence: 99%

Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo

Parte

González-Arribas

Diaz-Sanz

et al. 2021

Sci Rep

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Partial hepatectomy (PHx) is the gold standard for the treatment of colorectal cancer liver metastases. However, after removing a substantial amount of hepatic tissue, growth factors are released to induce liver regeneration, which may promote the proliferation of liver micrometastases or circulating tumour cells still present in the patient. The aim of this study is to assess the effect of PHx on the growth of liver metastases induced by intrasplenic cell inoculation as well as on in vitro proliferation of the same cancer cell line. Liver tumours were induced in 18 WAG/RijHsd male rats, by seeding 250,000 syngeneic colorectal cancer cells (CC531) into the spleen. The left lateral lobe of the liver was mobilized and in half of the animals it was removed to achieve a 40% hepatectomy. Twenty-eight days after tumour induction, the animals were sacrificed and the liver was removed and sliced to assess the relative tumour surface area (RTSA%). CC531 cells were cultured in presence of foetal calf serum, non-hepatectomised (NRS) or hepatectomized rat serum (HRS), and their proliferation rate at 24, 48, and 72 h was measured. RTSA% was significantly higher in animals which had undergone PHx than in the controls (non-hepatectomised) (46.98 ± 8.76% vs. 18.73 ± 5.65%; p < 0.05). Analysing each lobe separately, this difference in favour of hepatectomized animals was relevant and statistically significant in the paramedian and caudate lobes. But in the right lobe the difference was scarce and not significant. In vitro, 2.5% HRS achieved stronger proliferative rates than the control cultures (10% FCS) or their equivalent of NRS. In this experimental model, a parallelism has been shown between the effect of PHx on the growth of colorectal cancer cells in the liver and the effect of the serum on those cells in vitro.

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Section: Discussionmentioning

confidence: 99%

Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo

Parte

González-Arribas

Diaz-Sanz

et al. 2021

Sci Rep

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show abstract

“…Chemical induction has also been accepted as an appropriate tool for tumour induction. Various compounds, including dimethylhydrazine and its metabolites methylnitronitrosoguanidine, N-Nitroso-Nmethylurea and azoxymethane, have been shown to induce tumour and/or metastases growth in laboratory animals (32)(33)(34)(35)(36). These compounds have alkylating activity, which cause breakage of DNA chains, abnormal pairing of bases, and inhibition of cell division, nally, resulting in cell death.…”

Section: Experimental Tumour Induction Modelmentioning

confidence: 99%

Partial hepatectomy enhances growing of rat colorectal cancer cells CC531

Parte

González-Arribas

Diaz-Sanz

et al. 2020

Preprint

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Abstract Background Nowadays, partial hepatectomy (PHx) is the gold standard for the treatment of colorectal cancer liver metastases. After removing a substantial amount of hepatic tissue, however, growth factors are released to induce liver regeneration. These cytokines may promote the proliferation of liver micrometastases or circulating tumour cells still present in the patient. The aim of this study was to assess the effect of PHx on the growth of liver metastases after intrasplenic cell inoculation. Methods Liver tumours were induced in 18 WAG/RijHsd male rats, by seeding 250,000 syngeneic colorectal cancer cells (CC-531) into the spleen. Twenty-eight days after tumour induction, the animals were sacrificed and the liver was removed and sliced to assess the relative tumour surface area (RTSA%). Results The RTSA% was significantly higher in animals which had undergone PHx than the controls (not hepatectomised) (46.98 ± 8.76% vs. 18.73 ± 5.65%; p < 0.05). While no differences were observed in the right lateral lobe, in either hepatectomised or non-hepatectomised animals, there were significant differences in liver surface occupied by metastases in the paramedian and caudate lobes in both groups. Conclusion In this experimental model, the process of growth of colorectal cancer cells in the liver clearly accelerates following PHx.

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Cáncer de colon experimental: caracterización del modelo mediante marcadores tumorales y moléculas de adhesión

Cited by 2 publications

References 156 publications

Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo

Partial hepatectomy enhances the growth of CC531 rat colorectal cancer cells both in vitro and in vivo

Partial hepatectomy enhances growing of rat colorectal cancer cells CC531

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