Cancer Chemopreventive Potential of Luteolin-7-O-Glucoside Isolated From Ophiorrhiza mungos Linn

Abstract

The anticarcinogenic potential of the phytocompound Luteolin-7-O-Glucoside (LUT7G), isolated from the leaves of Ophiorrhiza mungos Linn, was studied against 4 different cancer cell lines (COLO 320 DM, AGS, MCF-7, and A549) and normal VERO cell line. The ability of LUT7G to induce apoptosis was determined by its antiradical activity, DNA fragmentation, expression of β-catenin, and chemopreventive efficacy in vivo by administering rats with DMH (20 mg/kg b.w., s.c.) for 4 consecutive wk and supplementing with 3 different doses throughout the experimental period of 16 wk. LUT7G scavenged 80% of DPPH radicals generated in vitro at 1000 μM and suppressed the expression of β-catenin to 40% at 120 μM concentrations. LUT7G induced apoptosis by scavenging ROS and suppressing the expression of β-catenin in COLO 320 DM cells and effectively inhibited ACF development in DMH-induced experimental carcinogenesis. Hence LUT7G can be a potent anticancer drug for colon carcinogenesis.

Luteolin, a Bioflavonoid Inhibits Colorectal Cancer through Modulation of Multiple Signaling Pathways: A Review

“…Luteolin also shown that; it inhibits the expression of cyclin D1, a downstream target of wnt/β-catenin pathway (Pandurangan et al, 2013a). Baskar et al, (2011) reported that luteolin-7-O-glucoside isolated from ophiorrhizamungos Linn showed to decrease the expression of β-catenin in COLO 320 DM cells.…”

Chemopreventive effects of caraway powder and oils to suppress 1, 2- dimethylhydrazine-induced colon carcinogenesis

“…In regards to the effect of DMH metabolite produced by CYP450 on beta-catenin gene expression [15,16,18,19], the data of this research together with our previous results [34,35] imply that feeding of rats with caraway essential oils and powder could modulate the enzymes interfere with DMH metabolism (GST and CYP1A1) leading to the decreased level of β-catenin gene expression. These entire events, finally leaded to the decreased level of ACF formation terminating in decreased progression of colon…”

“…A plant extract of Eleutherine palmifolia led to a significant decrease in the level of nuclear beta-catenin protein, resulted in the inhibitory effect on the transcription of TCF/beta-catenin in SW480 colon cancer cells in a dose-dependent manner together with selective cytotoxicity against three colorectal cancer cell lines [48]. Luteolin-7-O-Glucoside induced apoptosis by scavenging ROS and suppressing the expression of β-catenin in COLO 320 DM cells and effectively inhibited ACF development in DMH-induced experimental carcinogenesis [15]. Silibinin supplementation to DMH-treated rats restored the levels of GSH-dependent enzymes and decreased the levels of β-catenin, PCNA, argyrophilic nucleolar organizer regions and cyclin D1 [49].…”

“…In the progression of DMH carcinogenesis, the reactive metabolite causes the methylation of DNA in colonic epithelial cell leaded to β-catenin gene mutation [14][15][16][17][18][19]. β-catenin is a cadherin-binding protein that also functions as a transcriptional activator when complexes in the nucleus with members of the TCF/LEF family [20].…”

Luteoloside Inhibits Proliferation of Human Chronic Myeloid Leukemia K562 Cells by Inducing G2/M Phase Cell Cycle Arrest and Apoptosis

“…Luteoloside, a natural flavone subclass derived from plants, has exhibited a series of biological activities, such as potential antibacterial and antifungal [4], free radical scavenging [5], anti-oxidative capabilities [6,7], protection effects against doxorubicininduced cardiotoxicity [8], and anticarcinogenic potential against colon carcinogenesis [5] and hepatocellular carcinoma [9,10], etc. However, the effects of luteoloside on blood malignancy have not been reported.…”