Cancer Cell Surface Negative Charges: A Bio-Physical Manifestation of the Warburg Effect

Shi, Donglu

doi:10.1142/s1793984417710015

Cited by 25 publications

(14 citation statements)

References 14 publications

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“…It has been well-studied that the cancer cell surface is negatively charged due to secretion of lactate acid secretion [ 36 , 37 ]. Thus, the nanoparticle endocytosis may depend on nanomaterial size, surface charge, hydrophilicity, and concentration.…”

Section: Resultsmentioning

confidence: 99%

Assessing Suitability of Co@Au Core/Shell Nanoparticle Geometry for Improved Theranostics in Colon Carcinoma

et al. 2021

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The interactions between cells and nanomaterials at the nanoscale play a pivotal role in controlling cellular behavior and ample evidence links cell intercommunication to nanomaterial size. However, little is known about the effect of nanomaterial geometry on cell behavior. To elucidate this and to extend the application in cancer theranostics, we have engineered core–shell cobalt–gold nanoparticles with spherical (Co@Au NPs) and elliptical morphology (Co@Au NEs). Our results show that owing to superparamagnetism, Co@Au NPs can generate hyperthermia upon magnetic field stimulation. In contrast, due to the geometric difference, Co@Au NEs can be optically excited to generate hyperthermia upon photostimulation and elevate the medium temperature to 45 °C. Both nanomaterial geometries can be employed as prospective contrast agents; however, at identical concentration, Co@Au NPs exhibited 4-fold higher cytotoxicity to L929 fibroblasts as compared to Co@Au NEs, confirming the effect of nanomaterial geometry on cell fate. Furthermore, photostimulation-generated hyperthermia prompted detachment of anti-cancer drug, Methotrexate (MTX), from Co@Au NEs-MTX complex and which triggered 90% decrease in SW620 colon carcinoma cell viability, confirming their application in cancer theranostics. The geometry-based perturbation of cell fate can have a profound impact on our understanding of interactions at nano-bio interface which can be exploited for engineering materials with optimized geometries for superior theranostic applications.

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Section: Resultsmentioning

confidence: 99%

Assessing Suitability of Co@Au Core/Shell Nanoparticle Geometry for Improved Theranostics in Colon Carcinoma

et al. 2021

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“…We assume that, similar to proteins and RNA mentioned above, the negatively charged iodide ions are also bound to the cationic liposomal surface ( Figure 2 B) and are directly transported to the breast cancer cells via membrane fusion thus achieving high delivery efficiencies. However, cancer cells in general, have an overall negative surface charge and can be targeted by cationic nanoparticles very efficiently based on the attractive electrostatic forces between particle and cell membrane surfaces [ 31 , 32 ], we focus our statement exclusively on the breast cancer cell line used in this study.…”

Section: Discussionmentioning

confidence: 99%

Delivery of the Radionuclide 131I Using Cationic Fusogenic Liposomes as Nanocarriers

Kolašinac

Bier

Schmitt

et al. 2021

IJMS

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Liposomes are highly biocompatible and versatile drug carriers with an increasing number of applications in the field of nuclear medicine and diagnostics. So far, only negatively charged liposomes with intercalated radiometals, e.g., 64Cu, 99mTc, have been reported. However, the process of cellular uptake of liposomes by endocytosis is rather slow. Cellular uptake can be accelerated by recently developed cationic liposomes, which exhibit extraordinarily high membrane fusion ability. The aim of the present study was the development of the formulation and the characterization of such cationic fusogenic liposomes with intercalated radioactive [131I]I− for potential use in therapeutic applications. The epithelial human breast cancer cell line MDA-MB-231 was used as a model for invasive cancer cells and cellular uptake of [131I]I− was monitored in vitro. Delivery efficiencies of cationic and neutral liposomes were compared with uptake of free iodide. The best cargo delivery efficiency (~10%) was achieved using cationic fusogenic liposomes due to their special delivery pathway of membrane fusion. Additionally, human blood cells were also incubated with cationic control liposomes and free [131I]I−. In these cases, iodide delivery efficiencies remained below 3%.

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“…Surface charge of the biomaterials, especially the electrospun fibers, is most often tuned by surface chemical modifications [160]. Surface charge can be also exploited as a tool to selectively target tumoral cells due to their net negative charge; in fact, while physiological cells possess a neutral or slightly positive charge, tumoral cells possess a marked negative surface charge due to their metabolism, a phenomenon known as the Warburg effect [161]. In fact, tumoral cells' high glycolysis rate leads to an increase in the lactate acid production and a massive anions release; so, the cations removing results in a negative charge of the surface [162].…”

Section: Biological Advantages Of Cytocompatible Nanofibers-based Biomentioning

confidence: 99%

Topographical and Biomechanical Guidance of Electrospun Fibers for Biomedical Applications

et al. 2020

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Electrospinning is gaining increasing interest in the biomedical field as an eco-friendly and economic technique for production of random and oriented polymeric fibers. The aim of this review was to give an overview of electrospinning potentialities in the production of fibers for biomedical applications with a focus on the possibility to combine biomechanical and topographical stimuli. In fact, selection of the polymer and the eventual surface modification of the fibers allow selection of the proper chemical/biological signal to be administered to the cells. Moreover, a proper design of fiber orientation, dimension, and topography can give the opportunity to drive cell growth also from a spatial standpoint. At this purpose, the review contains a first introduction on potentialities of electrospinning for the obtainment of random and oriented fibers both with synthetic and natural polymers. The biological phenomena which can be guided and promoted by fibers composition and topography are in depth investigated and discussed in the second section of the paper. Finally, the recent strategies developed in the scientific community for the realization of electrospun fibers and for their surface modification for biomedical application are presented and discussed in the last section.

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Cancer Cell Surface Negative Charges: A Bio-Physical Manifestation of the Warburg Effect

Cited by 25 publications

References 14 publications

Assessing Suitability of Co@Au Core/Shell Nanoparticle Geometry for Improved Theranostics in Colon Carcinoma

Assessing Suitability of Co@Au Core/Shell Nanoparticle Geometry for Improved Theranostics in Colon Carcinoma

Delivery of the Radionuclide 131I Using Cationic Fusogenic Liposomes as Nanocarriers

Topographical and Biomechanical Guidance of Electrospun Fibers for Biomedical Applications

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