Cancer cell sensing and therapy using affinity tag-conjugated gold nanorods

Yasun, Emir; Kang, Houjun; Erdal, Huseyin; Cansız, Sena; Öçsoy, İsmail; Huang, Yu‐Fen; Tan, Weihong

doi:10.1098/rsfs.2013.0006

Cited by 49 publications

(36 citation statements)

References 62 publications

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“…For instance, gold and silver particles are widely used in biomedical applications [20][21][22][23][24][25][26][27] , especially for thiol linkers to selectively bind receptor proteins in targeted drug delivery application [28,29] . Therefore, if one can produce a metal shell, covering the polymer and sealing the capsules, the colloidosomes can be used for medical applications.…”

Section: Introductionmentioning

confidence: 99%

Aqueous core colloidosomes with a metal shell

Sun

Routh

2016

European Polymer Journal

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Colloidosomes can be used in a wide variety of industries, such as food, bioreactors and medicine. We report for the first time, aqueous core colloidosomes with a gold or silver shell. The diameter of the water core is from 0.7 µm to 2 µm. The formation of gold coated colloidosomes was achieved by using sodium citrate (Na 3 Citrate) in the core and gold chloride (HAuCl 4) in the wash solution. The gold shell colloidosomes are covered with spherical gold particles. Silver coated colloidosomes can be prepared by using L-Ascorbic acid in the core and silver nitrate (AgNO 3) in the wash solution. Various processing parameters result in different shell morphologies. The silver shell can seal the core of the microcapsule, and the encapsulated material can be released by the addition of nitric acid.

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Section: Introductionmentioning

confidence: 99%

Aqueous core colloidosomes with a metal shell

Sun

Routh

2016

European Polymer Journal

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“…1 For instance, the extinction cross-section coefficients of AuNRs are more than double those of gold nanoshells (AuNSs) 2 , the first example of gold nanoparticles (AuNPs) able to absorb in the NIR region. High absorption cross sections facilitate efficient energy absorption in the NIR, thus allowing equally efficient conversion of this absorbed energy to thermal energy.…”

Section: Introductionmentioning

confidence: 99%

BSA modification to reduce CTAB induced nonspecificity and cytotoxicity of aptamer-conjugated gold nanorods

Yasun

Barut

et al. 2015

Nanoscale

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Aptamer-conjugated gold nanorods (AuNRs) are excellent candidates for targeted hyperthermia therapy of cancer cells. However, in high concentrations of AuNRs, aptamer conjugation alone fails to result in highly cell-specific AuNRs due to the presence of positively charged cetyltrimethylammonium bromide (CTAB) as a templating surfactant. Besides causing nonspecific electrostatic interactions with the cell surfaces, CTAB can also be cytotoxic, leading to uncontrolled cell death. To avoid the nonspecific interactions and cytotoxicity triggered by CTAB, we report the further biologically inspired modification of aptamer-conjugated AuNRs with bovine serum albumin (BSA) protein. Following this modification, interaction between CTAB and the cell surface was efficiently blocked, thereby dramatically reducing the side effects of CTAB. This approach may provide a general and simple method to avoid one of the most serious issues in biomedical applications of nanomaterials: nonspecific binding of the nanomaterials with biological cells.

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“…[31][32][33][34][35][36][37] In this context, gold nanorods (Au NRs) have been receiving extensive attention, in large part owing to their strong extinction coefficient at longitudinal surface plasmon (LSP) resonance, which is dependent on their size, aspect ratio (AR) and refractive index of surrounding media. 39,[50][51][52][53][54][55][56][57] Those achievements have led to signicant advances in science and technology of LSPR sensors. 39,[50][51][52][53][54][55][56][57] Those achievements have led to signicant advances in science and technology of LSPR sensors.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric detection of glutathione based on transverse overgrowth of high aspect ratio gold nanorods investigated by MCR-ALS

2015

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In this paper, we present a simple platform for colorimetric detection of glutathione using gold nanorods (AR $ 6.5 AE 0.2) as a plasmonic sensor. The functional mechanism of the sensor is based on shifts of longitudinal plasmon resonance during selective transverse overgrowth induced by preferential binding of glutathione at the nanorod tips. Under the optimum conditions, a calibration curve showed two linear regimes at the range of 50 nM to 20 mM of glutathione with a detection limit as low as 40 nM. The nanosensor maintains relatively high selectivity for determination of glutathione in the presence of several other amino acids. However, cysteine at similar concentration levels strongly competes with glutathione for available binding sites, thereby interfering in the determination of glutathione. We employed multivariate curve resolution-alternating least square (MCR-ALS) to track the morphology variation of the nanorods and elucidate the kinetics of the overgrowth process in the presence of glutathione and cysteine.

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Cancer cell sensing and therapy using affinity tag-conjugated gold nanorods

Cited by 49 publications

References 62 publications

Aqueous core colloidosomes with a metal shell

Aqueous core colloidosomes with a metal shell

BSA modification to reduce CTAB induced nonspecificity and cytotoxicity of aptamer-conjugated gold nanorods

Colorimetric detection of glutathione based on transverse overgrowth of high aspect ratio gold nanorods investigated by MCR-ALS

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