Cancer cell-selective promoter recognition accompanies antitumor effect by glucocorticoid receptor-targeted gold nanoparticle

Sau, Samaresh; Agarwalla, Pritha; Mukherjee, Sudip; Bag, Indira; Sreedhar, B.; Pal‐Bhadra, Manika; Patra, Chitta Ranjan; Banerjee, Rajkumar

doi:10.1039/c4nr00974f

Cited by 50 publications

(49 citation statements)

References 36 publications

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“…Dynamic light scattering data showed that the average size (Z av ) of AuNPs and AuNPs‐Qu‐5 were 10.58 and 15.92 nm, respectively (Figure A,B). The comparatively large size of AuNPs‐Qu‐5 was observed in DLS indicated the attachment of the drug on gold nanoparticles surface . The size of AuNPs and AuNPs‐Qu‐5 acquired from DLS was slightly higher than that obtained from TEM.…”

Section: Resultsmentioning

confidence: 87%

“…The wavelength at maximum absorbance of AuNPs‐Qu‐5 showed considerable shift (λmax ~540 nm) from the naked AuNPs (λmax ~530 nm), indicating feasible attachment of the drug molecule on AuNPs surfaces (Figure A) . This result indicates the alteration of photo‐physical properties of AuNPs by the physical association of Qu, on gold nanoparticle surfaces …”

Section: Resultsmentioning

confidence: 92%

“…The comparatively large size of AuNPs-Qu-5 was observed in DLS indicated the attachment of the drug on gold nanoparticles surface. 32 The size of AuNPs and AuNPs-Qu-5 acquired from DLS was slightly higher than that obtained from TEM. This is probably due to the overall hydrodynamic size of Qu associated AuNPs obtained from DLS.…”

Section: Transmission Electron Microscopy and Dls Studiesmentioning

confidence: 78%

“…32 This result indicates the alteration of photo-physical properties of AuNPs by the physical association of Qu, on gold nanoparticle surfaces. 32 To determine the saturation concentration of Qu attached with AuNPs, a saturation curve of Qu was plotted in AuNPs supernatant (SI; Figure 1A). From the graph, it was observed that the absorbance of AuNPs reached its maximum value at the concentration of 5 μg/mL of Qu.…”

Section: Apoptosis By Annexin-v Fitc Assaymentioning

confidence: 90%

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Gold nanoparticles–conjugated quercetin induces apoptosis via inhibition of EGFR/PI3K/Akt–mediated pathway in breast cancer cell lines (MCF‐7 and MDA‐MB‐231)

Solaimuthu

Mukherjee

Das

et al. 2017

Cell Biochemistry & Function

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150

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Epidermal growth factor plays a major role in breast cancer cell proliferation, survival, and metastasis. Quercetin, a bioactive flavonoid, is shown to exhibit anticarcinogenic effects against various cancers including breast cancer. Hence, the present study was designed to evaluate the effects of gold nanoparticles-conjugated quercetin (AuNPs-Qu-5) in MCF-7 and MDA-MB-231 breast cancer cell lines. Borohydride reduced AuNPs were synthesized and conjugated with quercetin to yield AuNPs-Qu-5. Both were thoroughly characterized by several physicochemical techniques, and their cytotoxic effects were assessed by MTT assay. Apoptotic studies such as DAPI, AO/EtBr dual staining, and annexin V-FITC staining were performed. AuNPs and AuNPs-Qu-5 were spherical with crystalline nature, and the size of particles range from 3.0 to 4.5 nm. AuNPs-Qu-5 exhibited lower IC value compared to free Qu. There was a considerable increase in apoptotic population with increased nuclear condensation seen upon treatment with AuNPs-Qu-5. To delineate the molecular mechanism behind its apoptotic role, we analysed the proteins involved in apoptosis and epidermal growth factor receptor (EGFR)-mediated PI3K/Akt/GSK-3β signalling by immunoblotting and immunocytochemistry. The pro-apoptotic proteins (Bax, Caspase-3) were found to be up regulated and anti-apoptotic protein (Bcl-2) was down regulated on treatment with AuNPs-Qu-5. Additionally, AuNPs-Qu-5 treatment inhibited the EGFR and its downstream signalling molecules PI3K/Akt/mTOR/GSK-3β. In conclusion, administration of AuNPs-Qu-5 in breast cancer cell lines curtails cell proliferation through induction of apoptosis and also suppresses EGFR signalling. AuNPs-Qu-5 is more potent than free quercetin in causing cancer cell death, and hence, this could be a potential drug delivery system in breast cancer therapy.

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Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 92%

Section: Transmission Electron Microscopy and Dls Studiesmentioning

confidence: 78%

Section: Apoptosis By Annexin-v Fitc Assaymentioning

confidence: 90%

See 2 more Smart Citations

Gold nanoparticles–conjugated quercetin induces apoptosis via inhibition of EGFR/PI3K/Akt–mediated pathway in breast cancer cell lines (MCF‐7 and MDA‐MB‐231)

Solaimuthu

Mukherjee

Das

et al. 2017

Cell Biochemistry & Function

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150

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“…The membrane was again washed with TBS‐T and incubated with secondary antibody for 1 h at dark at room temperature. Finally, after washing the secondary antibody, blots were developed by colorimetric method using NBT/BCIP developing solution …”

Section: Methodsmentioning

confidence: 99%

Graphene Oxides Show Angiogenic Properties

Mukherjee

Sriram

Barui

et al. 2015

Adv Healthcare Materials

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188

116

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Angiogenesis, a process resulting in the formation of new capillaries from the pre-existing vasculature plays vital role for the development of therapeutic approaches for cancer, atherosclerosis, wound healing, and cardiovascular diseases. In this report, the synthesis, characterization, and angiogenic properties of graphene oxide (GO) and reduced graphene oxide (rGO) have been demonstrated, observed through several in vitro and in vivo angiogenesis assays. The results here demonstrate that the intracellular formation of reactive oxygen species and reactive nitrogen species as well as activation of phospho-eNOS and phospho-Akt might be the plausible mechanisms for GO and rGO induced angiogenesis. The results altogether suggest the possibilities for the development of alternative angiogenic therapeutic approach for the treatment of cardiovascular related diseases where angiogenesis plays a significant role.

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