Cancer-cell-membrane-camouflaged supramolecular self-assembly of antisense oligonucleotide and chemodrug for targeted combination therapy

Abstract

Anti-apoptotic proteins B-cell lymphoma-2 (Bcl-2) family proteins as critical regulators of cell death, overexpressed in many cancer cells, especially in multi-drug resistant cancer cells. Combinatorial gene and chemo therapies using...

Hybrid Membrane Camouflaged Chemodrug-Gene Nanoparticles for Enhanced Combination Therapy of Ovarian Cancer

“…In vitro, accumulated drug release of Mito-Her2@RSHM NPs aqueous solution in PBS (pH = 7.4 and pH = 5.5) was detected by UV spectrometer according to our previous study …”

“…The cellular uptake efficiency and cell apoptosis of Mito, Mito-Her2 NPs, Mito-Her2@SCM NPs, and Mito-Her2@RSHM NPs were analyzed by CLSM and flow cytometry according to the protocols in our previous report …”

“…Fabrication of RBCM/SCM Hybrid Membrane (RSHM). RBCM and SCM were obtained according to our previous study 41 and then mixed at the protein weight ratio of 1:1, sonicated at 37 °C, and extruded through 400 and 200 nm polycarbonate porous membranes to complete the membrane fusion. Finally, RSHM was collected by centrifugation (20000 rpm, 30 min, 4 °C) and resuspended in PBS.…”

“…The membrane protein profiles from Mito-Her2@RSHM NPs were evaluated by the SDS-PAGE method with RBCM, SCM, and RSHM as controls on the basis of our previous study. 41 Stability Measurement. Mito-Her2 NPs, Mito-Her2@SCM NPs, and Mito-Her2@RSHM NPs were suspended in PBS solution to monitor their stability over time.…”

“…In vitro, accumulated drug release of Mito-Her2@RSHM NPs aqueous solution in PBS (pH = 7.4 and pH = 5.5) was detected by UV spectrometer according to our previous study. 41 Colocalization Study of RSHM. The concentrations of RBCM and SCM membrane protein were quantified by a bicinchoninic acid protein kit.…”