Cancer cell detection in tissue sections using AFM

Lekka, Małgorzata; Gil, Dorota; Pogoda, Katarzyna; Dulińska-Litewka, Joanna; Jach, Robert; Gostek, Justyna; Klymenko, Olena; Prauzner-Bechcicki, Szymon; Stachura, Z.; Wiltowska-Zuber, Joanna; Okoń, Krzysztof; Laidler, Piotr

doi:10.1016/j.abb.2011.12.013

Cited by 311 publications

(280 citation statements)

References 22 publications

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“…Importantly, they mostly fall within the range (10 4 , 10 6 ) Hz, an interval compatible with LITUS, which is already widely employed for medical applications. Peak frequency values outside this interval are found for the sole cases associated with extreme limit situations, that is, both when the cells behave as fluid-like (Maxwell) viscoelastic materials and exhibit the bladder cells [29] kidney cells [32] breast cells [30] prostate cells [30] thyroid cells [33] mouse ovarian epithelia [34] breast cells [35] prostate cells [36] bladder cells [29] breast cells [28] breast cells [26] cells of the lung [26] Figure 6. Bar chart with a synopsis of the theoretically derived in-frequency responses of healthy and cancer cells whose mechanical properties have been experimentally measured: the histograms compare peak frequencies for each tumour and normal cell line pair examined, by averaging over all the results obtained from the six viscoelastic schemes used.…”

Section: Conclusion Limitations and Future Perspectivesmentioning

confidence: 99%

“…This implies that the physical measurements generally can be strongly dependent on the technique used and, as a consequence, quantitative estimations may cover wide ranges. Several experimental tests [26][27][28][29][30][31][32][33][34][35][36]53] have in fact shown that Young's modulus of the cytoplasm of different (healthy and cancer) cell lines can oscillate from about 100 Pa to 10 kPa. Also, the size of the cell nucleus is of the order of a few micrometres and may depend on the cell size [54].…”

Section: Sensitivity Analyses: Qualitative Insights Into and The Resomentioning

confidence: 99%

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A frequency-based hypothesis for mechanically targeting and selectively attacking cancer cells

Fraldi¹,

Cugno

Deseri

et al. 2015

J. R. Soc. Interface.

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Experimental studies recently performed on single cancer and healthy cells have demonstrated that the former are about 70% softer than the latter, regardless of the cell lines and the measurement technique used for determining the mechanical properties. At least in principle, the difference in cell stiffness might thus be exploited to create mechanical-based targeting strategies for discriminating neoplastic transformations within human cell populations and for designing innovative complementary tools to cell-specific molecular tumour markers, leading to possible applications in the diagnosis and treatment of cancer diseases. With the aim of characterizing and gaining insight into the overall frequency response of single-cell systems to mechanical stimuli (typically low-intensity therapeutic ultrasound), a generalized viscoelastic paradigm, combining classical and spring-pot-based models, is introduced for modelling this problem by neglecting the cascade of mechanobiological events involving the cell nucleus, cytoskeleton, elastic membrane and cytosol. Theoretical results show that differences in stiffness, experimentally observed ex vivo and in vitro, allow healthy and cancer cells to be discriminated, by highlighting frequencies (from tens to hundreds of kilohertz) associated with resonance-like phenomena-prevailing on thermal fluctuations-that could be helpful in targeting and selectively attacking tumour cells.

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Section: Conclusion Limitations and Future Perspectivesmentioning

confidence: 99%

Section: Sensitivity Analyses: Qualitative Insights Into and The Resomentioning

confidence: 99%

A frequency-based hypothesis for mechanically targeting and selectively attacking cancer cells

Fraldi¹,

Cugno

Deseri

et al. 2015

J. R. Soc. Interface.

View full text Add to dashboard Cite

show abstract

“…Plodinec et al [13] quantitatively measured the stiffness of normal, benign lesion, and malignant human breast tissues by combining AFM and H&E histological staining, showing that stiff profile of malignant tissue (several peaks including a prominent low-stiffness peak representative of cancer cells) was quite different from that of normal and benign tissues (a single peak). Lekka et al [62] also measured the elastic properties of patient tissues and found that cancer cells were softer than normal cells. Zhou et al [63] investigated the role of cellular mechanical properties in tongue squamous cell carcinoma (TSCC), showing that TSCC cells with higher metastatic potential showed decrease in the elastic modulus compared with TSCC cells with lower metastatic potential.…”

Section: Principle and Methods Of Afm Indentation Techniquesmentioning

confidence: 99%

Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy

Liu

et al. 2014

Chin. Sci. Bull.

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The advent of atomic force microscopy (AFM) provides a powerful tool for investigating the behaviors of single living cells under near physiological conditions. Besides acquiring the images of cellular ultra-microstructures with nanometer resolution, the most remarkable advances are achieved on the use of AFM indenting technique to quantify the mechanical properties of single living cells. By indenting single living cells with AFM tip, we can obtain the mechanical properties of cells and monitor their dynamic changes during the biological processes (e.g., after the stimulation of drugs). AFM indentation-based mechanical analysis of single cells provides a novel approach to characterize the behaviors of cells from the perspective of biomechanics, considerably complementing the traditional biological experimental methods. Now, AFM indentation technique has been widely used in the life sciences, yielding a large amount of novel information that is meaningful to our understanding of the underlying mechanisms that govern the cellular biological functions. Here, based on the authors' own researches on AFM measurement of cellular mechanical properties, the principle and method of AFM indentation technique was presented, the recent progress of measuring the cellular mechanical properties using AFM was summarized, and the challenges of AFM single-cell nanomechanical analysis were discussed.

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“…Hence the behaviors of the receptors on the cells grown in vitro may not reflect the real situation in vivo. Recently using AFM to directly investigate the properties (such as the cellular elasticity) of cancer cells from patients has been reported [53,54]. To our knowledge, using AFM to directly investigating the target proteins on the surface of cancer cells from patients has not Fig.…”

Section: E X P E R I M E N T a L C E L L R E S E A R Cmentioning

confidence: 99%

Nanoscale mapping and organization analysis of target proteins on cancer cells from B-cell lymphoma patients

Xiao

Liu

et al. 2013

Experimental Cell Research

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Cancer cell detection in tissue sections using AFM

Cited by 311 publications

References 22 publications

A frequency-based hypothesis for mechanically targeting and selectively attacking cancer cells

A frequency-based hypothesis for mechanically targeting and selectively attacking cancer cells

Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy

Nanoscale mapping and organization analysis of target proteins on cancer cells from B-cell lymphoma patients

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