Cancer Biomarkers for Diagnosis, Prognosis and Therapy

Barh, Debmalya; Agte, Vaishali; Dhawan, Dipali; Agte, Varsha; Padh, Harish

doi:10.1002/9781119967309.ch2

Cited by 3 publications

(3 citation statements)

References 188 publications

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“…To this end, several biomarkers through SNP studies have revealed an association with cancer and many other complex traits. 66 In search for the common CNVs, which are associated with malignancy, a map was created that cataloged all the known CNVs whose loci coincide with that of the already known cancer-related genes. Upon analysis it was found that 49 cancer genes directly encompassed or overlapped by CNV in more than one person in a large reference population.…”

Section: Cnv and Susceptibility To Other Common Disorders Hiv/aids Sumentioning

confidence: 99%

Implications of gene copy-number variation in health and diseases

2011

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Section: Cnv and Susceptibility To Other Common Disorders Hiv/aids Sumentioning

confidence: 99%

Implications of gene copy-number variation in health and diseases

2011

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“…Diagnostic biomarkers are a key element in research, diagnostics and targeted therapeutics [1,2]. Recently, great emphasis has being placed on identifying circulating biomarkers, especially present in blood ( plasma and serum), able to provide information on the body's response to cancer, as well as on the relationship between a tumour cell development and its environment [3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Integration of binding peptide selection and multifunctional particles as tool-box for capture of soluble proteins in serum

Cusano

Causa

Moglie

et al. 2014

J. R. Soc. Interface.

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In this paper, we report on a general approach for the detection of a specific tumoural biomarker directly in serum. Such detection is made possible using a protein-binding peptide selected through an improved phage display technique and then conjugated to engineered microparticles (MPs). Protein biomarkers represent an unlimited source of information for non-invasive diagnostic and prognostic tests; MP-based assays are becoming largely used in manipulation of soluble biomarkers, but their direct use in serum is hampered by the complex biomolecular environment. Our technique overcomes the current limitations as it produces a selective MP-engineered with an antifouling layer-that 'captures' the relevant protein staying impervious to the background. Our system succeeds in fishing-out the human tumour necrosis factor alpha directly in serum with a high selectivity degree. Our method could have great impact in soluble protein manipulation and detection for a wide variety of diagnostic applications.

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“…The fold/level of expression of miRNA in oral malignancies is different from that of normal tissues. 8 The evidence indicates that changes in the miRNA profile may be identified in saliva that is locally absorbed by malignant cells, and that several techniques may be employed to identify the miRNA secreted in saliva.…”

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confidence: 99%

The Role of Salivary Micro RNAs as a Diagnostic Marker in Early Detection of Oral Squamous Cell Carcinoma

Munwar Baloch,

Sana Javed Khoso,

Saima Saman

et al. 2024

Ann Pak Inst Med Sci

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OBJECTIVE: To assess the diagnostic role of salivary microRNA for early detection oforal squamous cell carcinoma and to compare the salivary microRNA with Biopsy in thediagnosis of oral squamous cell carcinoma.Methodology: The descriptive cross-sectional study was conducted in the department of pathology and molecular laboratory, department of oral & maxillofacial surgery of Liaquat University of Medical Health Sciences, Jamshoro / Hyderabad.Inclusion Criteria: All histopathologicaly diagnosed OSCC patients of both genders with age group 18 years and above will be included in the study after taking their informed consent. Whole saliva samples (inactivated) were collectedfrom subjects diagnosed as OSCC and controls. The subjects were counseled to avoid eating, drinking, smoking or oral procedures for at least 1 h prior to the collection of saliva. Subjects are asked to rinse their mouth well with distilled drinking water for one minute before taking the saliva samples. After five minutes of oral rinsing spit 5 mL of saliva into a 50 mL sterile tube placed on the ice. The tube should remain on ice while collecting the saliva samples. Fourhundred microliters of the whole saliva mixture (200 ?L whole saliva and 200 ?L RNA later),and 400 ?L of the supernatant saliva was used for RNA extraction. Saliva samples wereextracted using the mirVana™ miRNA Isolation Kit according to the manufacturer's guideline(Ambion Inc., Austin, TX). Whole saliva samples were preserved with RN Alater (QIAGENInc., Valencia, CA) and supernatant saliva samples were preserved with SUPE Rase. In™ (Ambion Inc., Austin, TX). The data was entered in the statistical package for social sciences for windows (SPSS) V: 26.RESULTS: A total of 120 samples of oral squamous cell carcinoma were taken, which met theinclusion criteria. Mean age of the patients was 47.45+10.85 years. 85(70.8%) were married and35 (29.2%) were unmarried. 90.0% were married and 5.0% were unmarried, while 5.0% werewidow. Cigarette smoking, paan, chaalia and naswar were the commonest cause of the oralsquamous cell carcinoma. Lips, buccal mucosa and tong were the commonest t sites of the oralsquamous cell carcinoma. 16.7% cases had positive family history of oral squamous cellcarcinoma. Out of all study subjects, most of the cases 59.2% had moderately differentiatedSCC, 30.0% cases had well-differentiated SCC and 10.8% of the cases had poor differentiatedSCC. Out of all study subjects, 87.5% of the patients had positive micro-RNA expression.Micro-RNA expression was significantly associated, with poorly differentiated squamous cellcarcinoma (p=0.016). CONCLUSION: Study revealed that the salivary miroRNA expression were significantlypositive in patients of OSCC. Therefore, salivary microRNA could be considered as a useful andadvantageous biomarker for the detection and tracking of OSCC across various levels of tissueabnormalities

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Cancer Biomarkers for Diagnosis, Prognosis and Therapy

Cited by 3 publications

References 188 publications

Implications of gene copy-number variation in health and diseases

Implications of gene copy-number variation in health and diseases

Integration of binding peptide selection and multifunctional particles as tool-box for capture of soluble proteins in serum

The Role of Salivary Micro RNAs as a Diagnostic Marker in Early Detection of Oral Squamous Cell Carcinoma

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