Cancer and the Search for Selective Biochemical Inhibitors

Hoffman, E.

doi:10.1201/9781420045963

Cited by 13 publications

(9 citation statements)

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“…Therefore, there have been several studies to find new anticancer compounds (1). It has been shown that plants provide important resources of active anticancer ingredients persuading scientists to focus on their anti-tumor agents (234). Colchicine, vincristine, vinblastine, and paclitaxel are some important anticancer drugs with plant origin (35).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic effects of Pinus eldarica essential oil and extracts on HeLa and MCF-7 cell lines

2016

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Several attempts have so far been made in the search of new anticancer agents of plant origin. Some studies have reported that different species of Pine genus possess cytotoxic activities against various cancer cell lines. In the present study, we evaluated the cytotoxic effects of Pinus eldarica bark and leaf extracts or leaf essential oil on HeLa and MCF-7 tumor cell lines. Hydroalcoholic and phenolic extracts and the essential oil of plant were prepared. Total phenolic contents of the extracts were measured using Folin-Ciocalteu reagent. Essential oil components were determined by gas chromatography-mass spectroscopy (GC-MS). Cytotoxic activity of the extracts and essential oil against HeLa and MCF-7 tumor cell lines were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The polyphenolic content of hydroalcoholic and phenolic extracts of the bark and hydroalcoholic extract of the leaf were 48.31%, 47.2%, and 8.47%, respectively. According to the GC-MS analysis, the major components of the leaf oil of P. eldarica were: β -caryophyllene (14.8%), germacrene D (12.9%), α–terpinenyl acetate (8.15%), α –pinene (5.7%), and –α humulene (5.9%). Bark extracts and leaf essential oil of P. eldarica significantly reduced the viability of both HeLa and MCF-7 cells in a concentration dependent manner. However, leaf extract showed less inhibitory effects against both cell lines. The essential oil of P. eldarica was more cytotoxic than its hydroalcoholic and phenolic extracts. The terpenes and phenolic compounds were probably responsible for cytotoxicity of P. eldarica. Therefore, P. eldarica might have a good potential for active anticancer agents.

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Section: Introductionmentioning

confidence: 99%

Cytotoxic effects of Pinus eldarica essential oil and extracts on HeLa and MCF-7 cell lines

2016

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“…The fruiting body of Lactarius piperatus that is a member of Russulaceae family is a creamy‐white mushroom that is funnel‐shaped when mature, with exceptionally crowded gills. In more recent times, it has been found that L. piperatus can be used as an antiviral agent, and the latex has been used against viral warts (Hoffman 1999).…”

Section: Introductionmentioning

confidence: 99%

Purification and Characterization of a Mushroom Polyphenol Oxidase and Its Activity in Organic Solvents

Öz¹,

Çolak²,

Özel³

et al. 2011

Journal of Food Biochemistry

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Polyphenol oxidase (PPO) was purified from Lactarius piperatus (L.) Pers. by using Sepharose 4B‐L‐tyrosine‐p‐amino benzoic acid affinity column. Optimum pH and temperature of purified PPOs of L. piperatus were found to be 7.0 and 20C, respectively, by using catechol as a substrate. The enzyme retained 100% of its original activity at 4C and its optimum pH value for 24 and 72 h. L. piperatus PPO was also quite stable at 20C after 4 h incubation. The Km and Vmax values were calculated as 1 mM and 25 U/mg protein, respectively. Ascorbic acid was found to be the most potent inhibitor for the enzyme. The mushroom PPO was an effective biocatalyst in the selected organic solvents such as dichloromethane, heptane and toluene when using catechin as a substrate. All data support that L. piperatus has a highly active PPO possessing similar biochemical and kinetic characteristics to some plant PPO enzymes. PRACTICAL APPLICATIONS Polyphenol oxidases (PPOs) are a group of copper‐containing enzymes that are widely distributed from bacteria to mammals. Some different mushroom PPOs are subjected to further characterization studies in terms of their biochemical characteristics and their potentials in biotechnological applications. So, purification and characterization studies for PPOs from new sources are very important to explain their biochemical properties and behavior. Thus, one more enzyme may also find applications in food or drug industries.

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“…Secara empirik tanaman dari keluarga gaultheria telah digunakan dalam pengobatan kanker dan leukimia (Hoffman, 2007 (Kusumo dkk., 2013). …”

Section: Pendahuluanunclassified

Optimasi Produksi Gaultherin Dari Gandapura Dengan Teknologi Mixed-Drying Extraction

Kusumo¹

2016

JTI

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Cancer and the Search for Selective Biochemical Inhibitors

Cited by 13 publications

References 0 publications

Cytotoxic effects of Pinus eldarica essential oil and extracts on HeLa and MCF-7 cell lines

Cytotoxic effects of Pinus eldarica essential oil and extracts on HeLa and MCF-7 cell lines

Purification and Characterization of a Mushroom Polyphenol Oxidase and Its Activity in Organic Solvents

Optimasi Produksi Gaultherin Dari Gandapura Dengan Teknologi Mixed-Drying Extraction

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