Canalicular system reorganization during mouse platelet activation as revealed by 3D ultrastructural analysis

Pokrovskaya, Irina D.; Tobin, Michael P.; Desai, Rohan; Joshi, Smita; Kamykowski, Jeffrey A.; Aronova, Maria A.; Whiteheart, Sidney W.; Leapman, Richard D.; Storrie, Brian

doi:10.1080/09537104.2020.1719993

Cited by 8 publications

(8 citation statements)

References 21 publications

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“…Activation process increases the intracellular concentration of calcium ions-by releasing from specific cytoskeleton structures and calcium influx across cell membrane. Elevated calcium concentration results in ultrastructural (morphological) and functional Plt alterations [17,[30][31][32][33].…”

Section: Platelet Morphology Ultrastructure and Functionalitymentioning

confidence: 99%

“…Elevated intracellular calcium concentration stimulates the phospholipase A2 (which releases arachidonic acid from membrane phospholipids). Arachidonic acid is then metabolized lastly into TXA2-a potent Plt activator [6,[31][32][33][34][35][36][37].…”

Section: Platelet Morphology Ultrastructure and Functionalitymentioning

confidence: 99%

“…Also, it incorporates various absorbed coagulation factors and receptors for Rapid Cytoreduction by Plateletapheresis in the Treatment of Thrombocythemia DOI: http://dx.doi.org/10.5772/intechopen.93158 thrombin, ADP, collagen, vWF, thrombospondin, TXA2, prostacyclin, fibrinogen, fibronectin, etc. [18,[31][32][33].…”

Section: Platelet Morphology Ultrastructure and Functionalitymentioning

confidence: 99%

“…In the microfilaments actin, myosin, actomyosin, and other substances were detected. Actin microfilaments in the "sol-gel zone" form actin cytoskeleton (matrix for organelles), while actomyosin is responsible for Plt contraction and may promote granule secretion [6,[32][33][34][35].…”

Section: Platelet Morphology Ultrastructure and Functionalitymentioning

confidence: 99%

“…Finally, the mitochondria are also in the "organelle zone" and provide Plt energy requirements. They are also a deposit and an important discharger of calcium ions [17,[30][31][32][33][34][35].…”

Section: Platelet Morphology Ultrastructure and Functionalitymentioning

confidence: 99%

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Rapid Cytoreduction by Plateletapheresis in the Treatment of Thrombocythemia

Balint¹,

Pavlović²,

Todorović³

2020

Platelets

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The objective of this chapter is to provide a systematic overview of current knowledge regarding therapeutic apheresis-primarily therapeutic plateletapheresis (TP)-and to summarize evidence-based practical approaches related to cytapheresis treatment of "hyperthrombocytosis" or "extreme thrombocytosis" (ETC). Our results of platelet (Plt) quantitative/qualitative analyses and evaluation of efficacy of apheresis systems/devices-on the basis of Plt removal and in vivo Plt depletion-will be presented. Our preclinical researches confirmed that in Plt concentrates, the initial ratio of discoid shapes was 70%, spherical 20%, and less valuable (dendritic/balloonized) shapes 10%-with morphological score of platelets (MSP = 300-400). After storage, the ratio of discoid and spherical shapes was decreased, while the less valuable ones progressively increased (MSP = 200). Electron microscopy has shown discoid shapes with typical ultrastructural properties. Spherical shapes with reduced electron density and peripheral location of granules/organelles were detected. Also, dendritic shapes with cytoskeletal "rearrangement," membrane system integrity damages, and pseudopodia formations were documented. Our clinical study demonstrated that TP was useful in ETC treatment and should help prevention of "thrombo-hemorrhagic" events-until chemotherapy, antiplatelet drugs, and other medication take effect. During TP treatment, Plt count and morphology/ultrastructure were examined. Plt functions by multiplate analyzer were evaluated. We concluded that intensive TP was an effective, safe, and rapid cytoreductive treatment for ET.

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Section: Platelet Morphology Ultrastructure and Functionalitymentioning

confidence: 99%

Section: Platelet Morphology Ultrastructure and Functionalitymentioning

confidence: 99%

Section: Platelet Morphology Ultrastructure and Functionalitymentioning

confidence: 99%

Section: Platelet Morphology Ultrastructure and Functionalitymentioning

confidence: 99%

Section: Platelet Morphology Ultrastructure and Functionalitymentioning

confidence: 99%

See 3 more Smart Citations

Rapid Cytoreduction by Plateletapheresis in the Treatment of Thrombocythemia

Balint¹,

Pavlović²,

Todorović³

2020

Platelets

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High-Pressure Freezing Followed by Freeze Substitution: An Optimal Electron Microscope Technique to Study Golgi Apparatus Organization and Membrane Trafficking

Liu

Pokrovskaya

Storrie

2022

Methods in Molecular Biology

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Megapinosomes and homologous structures in hematopoietic cells

Bauer

Frascaroli

Walther

2022

Histochem Cell Biol

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Megapinosomes are endocytic organelles found in human macrophage colony-stimulating factor (M-CSF) monocyte-derived M macrophages. They are large (several microns) and have a complex internal structure that is connected with the cytosol and consists of interconnected knots and concave bridges with sizes in the range of 100 nm. We called this structure trabecular meshwork. The luminal part of the megapinosome can be connected with luminal tubules and cisterns that form the megapinosome complex. The structures are especially well visible in scanning electron tomography when macrophages are prepared by high-pressure freezing and freeze substitution. Our research received a new impulse after studying the literature on hematopoietic cells, where very similar, most likely homologous, structures have been published in peritoneal macrophages as well as in megakaryocytes and blood platelets. In platelets, they serve as membrane storage that is used for structural changes of platelets during activation.

show abstract

Canalicular system reorganization during mouse platelet activation as revealed by 3D ultrastructural analysis

Cited by 8 publications

References 21 publications

Rapid Cytoreduction by Plateletapheresis in the Treatment of Thrombocythemia

Rapid Cytoreduction by Plateletapheresis in the Treatment of Thrombocythemia

High-Pressure Freezing Followed by Freeze Substitution: An Optimal Electron Microscope Technique to Study Golgi Apparatus Organization and Membrane Trafficking

Megapinosomes and homologous structures in hematopoietic cells

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