Can xylene and quercetin directly affect basic ovarian cell functions?

Tarko, Adam; Štochmaľová, Aneta; Hrabovszká, Sandra; Vachanová, A; Harrath, Abdel Halim; Alwasel, Saleh; Grossman, R.; Sirotkin, Alexander V.

doi:10.1016/j.rvsc.2018.07.010

Cited by 23 publications

(43 citation statements)

References 22 publications

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“…However, only a small proportion of their effects was found to be similar, probably because of the participation of other molecules or molecule complexes. The plants effect could not be explained by the presence of another ubiquitous polyphenolic phytochemical, quercetin, because its suppressive action on all the ovarian functions reported previously [ 58 , 59 , 60 , 61 ] is opposed to that observed for buckwheat and vitex in the present study. Therefore, it seems that rutin, apigenin, and quercetin cannot explain the action of buckwheat or vitex on ovarian cells, and that these polyphenols cannot replace the treatment with the whole plant extract.…”

Section: Discussioncontrasting

confidence: 97%

“…The granulosa cells were isolated from the ovaries of non-cycling pubertal gilts (180 days age, approximately), and were processed and cultured as previously described [ 57 , 58 , 59 , 60 , 61 ]. Briefly, the collected granulosa cells (at 10 6 cells/mL concentration) were precultured in sterile Dulbecco’s modified Eagle medium (DMEM)/F12 1:1, containing 10% fetal calf serum (both from BioWhittakerTM, Verviers, Belgium) and 1% antibiotic-antimycotic solution (Sigma-Aldrich, St. Louis, MO, USA) in 16-well (200 µL/well) chamber slides (Nunc Inc., International, Naperville, IL, USA) during 3–4 days.…”

Section: Methodsmentioning

confidence: 99%

“…In this case, the plant additive was first added to the cell culture and, after 10–15 min, CuNPs/TiO 2 was added to the resulting medium. These doses correspond to those of CuNPs that can be ingested and that have been applied in previous animal experiments, both in vivo and in vitro [ 20 , 58 , 59 , 60 , 61 ]. The dose of the plant additives was selected on the basis of the doses used in the corresponding previous in vitro experiments [ 41 , 43 , 45 , 49 , 50 ].…”

Section: Methodsmentioning

confidence: 99%

“…Immunocytochemistry was used to detect the presence of PCNA and bax in the cells, as described previously [ 19 , 20 , 57 , 58 , 59 , 60 , 61 ], by means of primary monoclonal antibodies against PCNA and bax (dilution 1:500; from Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), secondary swine antibodies against mouse IgG labeled with horseradish peroxidase (dilution 1:1000; Servac, Prague, Czech Republic) and visualized by DAB-substrate staining (Roche Diagnostics GmbH, Manheim, Germany). In some cases, the assay was validated by these primary antibodies and secondary polyclonal goat antibodies against mouse IgGs, labeled with fluorescein isothiocyanate (FITC, dilution 1:1000; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA; Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

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Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals

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The application of nanoparticles has experienced a vertiginous growth, but their interaction with food and medicinal plants in organisms, especially in the control of reproduction, remains unresolved. We examined the influence of copper nanoparticles supported on titania (CuNPs/TiO2), plant extracts (buckwheat (Fagopyrum esculentum) and vitex (Vitex agnus-castus)), phytochemicals (rutin and apigenin), and their combination with CuNPs/TiO2 on ovarian cell functions, using cultured porcine ovarian granulosa cells. Cell viability, proliferation (PCNA accumulation), apoptosis (accumulation of bax), and hormones release (progesterone, testosterone, and 17β-estradiol) were analyzed by the Trypan blue test, quantitative immunocytochemistry, and ELISA, respectively. CuNPs/TiO2 increased cell viability, proliferation, apoptosis, and testosterone but not progesterone release, and reduced the 17β-estradiol output. Plant extracts and components have similar stimulatory action on ovarian cell functions as CuNPs/TiO2, but abated the majority of the CuNPs/TiO2 effects. This study concludes that (1) CuNPs/TiO2 can directly stimulate ovarian cell functions, promoting ovarian cell proliferation, apoptosis, turnover, viability, and steroid hormones release; (2) the plants buckwheat and vitex, as well as rutin and apigenin, can promote some of these ovarian functions too; and (3) these plant additives mitigate the CuNPs/TiO2’s activity, something that must be considered when applied together.

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Section: Discussioncontrasting

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals

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“…The folk traditions, influence of eastern medicine, lack of satisfaction with synthetic pharmacological drugs and food producers are promoting the wide application of some plants containing phytoestrogens and antioxidants for improvement of not only metabolism but also of reproduction and fertility. The corresponding studies however demonstrated the ability of popular green tea (Baláži et al 2019), turmeric/curcumin (Kádasi et al 2017), rooibos, ginkgo (Štochmaľová et al 2018), fennel (Tarko et al 2020), flaxseed (Štochmaľová et al 2019), and their constituent phytoestrogen quercetin (Tarko et al 2018(Tarko et al , 2019Sirotkin et al 2019a,b) not improve, but directly suppress animal and human ovarian cell functions. These unexpected data should be taken into account by the consumption and overconsumption of these popular plants.…”

Section: Mythic Interconnectionmentioning

confidence: 99%

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The cross-cutting theme of the 3/2 issue of Researcher is the problem of transformation of human nature in civilizational, historical, and cultural dimensions. The opening essay by Ilia Delio focuses on the current semantic context of this transformation and its cons equences, which change taken for granted values in daily life. The author announces the end of an era that affirms the value of an autonomous liberal subject, and connects the future with the beginning of the history of post-human being, a new type of personality integrated into the "maps of connections" (through socialism, pol itics, environment, economics, sexuality, etc.). Arkady Ursul develops the topic of information transformation, which shows that essence of globalization is the approval of the tr iad of universals: spatial distribution, integration and universality. Through the prism of these universals, the author considers cultu ral genesis and predicts the "global developmental algorithms" of man in the information world. Konstantin Kolin analyzes the main challenges of modern civil ization that should be overcome in the process of globalist discourse of civilizational transformation. The author includes the geopolit ical concept of "nuclear deterrence", the ideology of post-industrial society, the exhaustion of natural resources, and the development of artificial intelligence to these challenges. The answer to these challenges should be a new enlightenment in which the focus is on caring for the future of the planet, and on finding a balance in sol ving global problems. An essay by Italian researcher of Russian history, Ornella Calvarese, is dedicated to the wandering bards of ancient Russiakaliki. Turning to the study of a Russian Middle Ages phenomenon, the author draws a conclusion about the cultural role of spiritual nomadism, which has influenced and still continues to influence the Russian worldview. A deep historical perspective allows us to recreate the past, reveal hidden meanings, restore national identity against the background of spasmodic, often interrupted stages of transformation of Russian culture. We continue to publish Nadezhda Gonotskaya's research piece "Recipe: the Apotheosis of Rationality or the Magic of Life?". In this second part of her essay, attention is paid to a special case of

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Buckwheat, rooibos, and vitex extracts can mitigate adverse effects of xylene on ovarian cells in vitro

Sirotkin

Macejková

Tarko

et al. 2020

Environ Sci Pollut Res

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This study examines whether selected functional food and medicinal plants can mitigate the adverse effects of xylene on ovarian cells. The influences of xylene (0, 10, 100, or 1000 ng/mL), buckwheat (Fagopyrum esculentum), rooibos (Aspalathus linearis), vitex (Vitex agnus-castus), extracts (10 μg/mL each), and a combination of xylene with these plant additives on cultured porcine ovarian granulosa cells are compared. Cell viability, proliferation (PCNA accumulation), apoptosis (accumulation of bax), and release of progesterone (P4) and estradiol (E2) were analyzed by the trypan blue tests, quantitative immunocytochemistry, and enzyme-linked immunosorbent assays, respectively. Xylene suppressed all measures of ovarian cell function. Rooibos prevented all of xylene's effects, whereas buckwheat and vitex prevented four of five of the analyzed effects (buckwheat prevented xylene influence on viability, PCNA, bax, and E2; vitex prevented xylene action on viability, PCNA, and P4 and E2). These observations show that xylene has the potential to suppress ovarian cell functions, and that buckwheat, rooibos, and vitex can mitigate those effects, making them natural protectors against the adverse effects of xylene on ovarian cells.

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Can xylene and quercetin directly affect basic ovarian cell functions?

Cited by 23 publications

References 22 publications

Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals

Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals

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Buckwheat, rooibos, and vitex extracts can mitigate adverse effects of xylene on ovarian cells in vitro

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