Can we Achieve the Perfect Injectable Scaffold for Cell Therapy?

Guyot, Capucine; Lerouge, Sophie

doi:10.4155/fsoa-2017-0153

Cited by 13 publications

(12 citation statements)

References 20 publications

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“…Although AM techniques are first to come to the mind to fabricate defect matching scaffolds, their use requires highly accurate imaging techniques to be able to create 3D custom-made models (Moglia et al, 2014b). Alternatively, injectable materials that harden in situ can fill irregular shapes by minimal invasive delivery ( Figure 10D), and they can also be used as a carrier for cells and other biological molecules (Temenoff and Mikos, 2000;Chang et al, 2017;Guyot and Lerouge, 2018).…”

Section: Injectingmentioning

confidence: 99%

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Dikici

Claeyssens

2020

Front. Bioeng. Biotechnol.

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Tissue engineering (TE) aims to regenerate critical size defects, which cannot heal naturally, by using highly porous matrices called TE scaffolds made of biocompatible and biodegradable materials. There are various manufacturing techniques commonly used to fabricate TE scaffolds. However, in most cases, they do not provide materials with a highly interconnected pore design. Thus, emulsion templating is a promising and convenient route for the fabrication of matrices with up to 99% porosity and high interconnectivity. These matrices have been used for various application areas for decades. Although this polymer structuring technique is older than TE itself, the use of polymerised internal phase emulsions (PolyHIPEs) in TE is relatively new compared to other scaffold manufacturing techniques. It is likely because it requires a multidisciplinary background including materials science, chemistry and TE although producing emulsion templated scaffolds is practically simple. To date, a number of excellent reviews on emulsion templating have been published by the pioneers in this field in order to explain the chemistry behind this technique and potential areas of use of the emulsion templated structures. This particular review focusses on the key points of how emulsion templated scaffolds can be fabricated for different TE applications. Accordingly, we first explain the basics of emulsion templating and characteristics of PolyHIPE scaffolds. Then, we discuss the role of each ingredient in the emulsion and the impact of the compositional changes and process conditions on the characteristics of PolyHIPEs. Afterward, current fabrication methods of biocompatible PolyHIPE scaffolds and polymerisation routes are detailed, and the functionalisation strategies that can be used to improve the biological activity of PolyHIPE scaffolds are discussed. Finally, the applications of PolyHIPEs on soft and hard TE as well as in vitro models and drug delivery in the literature are summarised.

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Section: Injectingmentioning

confidence: 99%

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Dikici

Claeyssens

2020

Front. Bioeng. Biotechnol.

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“…The use of hydrogels to treat vocal fold scarring has the potential to improve patient therapeutic outcomes compared to current clinical practices 1,64 . When developing vocal fold hydrogels, it is necessary to establish the material composition that minimizes undesirable inflammation, maintains optimum mechanical integrity, and promotes beneficial immunomodulatory effects 48,55 . Hydrogel stiffness is a tunable material property that can be harnessed to modulate the immune response 4,46,65,66 .…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, increased glyoxal content within glycol‐chitosan hydrogels has previously been associated with reduced VFF viability 14 . As such, an appropriate glyoxal concentration must be selected that provides the hydrogel with mechanical integrity and relevant stiffness while minimizing cytotoxic effects upon cells 54,55 …”

Section: Introductionmentioning

confidence: 99%

An in vitro assessment of the response of THP‐1 macrophages to varying stiffness of a glycol‐chitosan hydrogel for vocal fold tissue engineering applications

Coburn

Herbay

Berrini

et al. 2020

J Biomedical Materials Res

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The physical properties of a biomaterial play an essential role in regulating immune and reparative activities within the host tissue. This study aimed to evaluate the immunological impact of material stiffness of a glycol‐chitosan hydrogel designed for vocal fold tissue engineering. Hydrogel stiffness was varied via the concentration of glyoxal cross‐linker applied. Hydrogel mechanical properties were characterized through atomic force microscopy and shear plate rheometry. Using a transwell setup, macrophages were co‐cultured with human vocal fold fibroblasts that were embedded within the hydrogel. Macrophage viability and cytokine secretion were evaluated at 3, 24, and 72 hr of culture. Flow cytometry was applied to evaluate macrophage cell surface markers after 72 hr of cell culture. Results indicated that increasing hydrogel stiffness was associated with increased anti‐inflammatory activity compared to relevant controls. In addition, increased anti‐inflammatory activity was observed in hydrogel co‐cultures. This study highlighted the importance of hydrogel stiffness from an immunological viewpoint when designing novel vocal fold hydrogels.

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“…Finally, many scientists have taken approaches to incorporate injectable systems into their regenerative strategies. Injectable systems are attractive because they are minimally invasive, allow for decreased patient recovery time, flow to fill the defect site, and reduce the risk of surgical site infection [ 52 ]. Many of the injectable strategies used for skeletal muscle engineering rely on a thermoresponsive hydrogel systems that gel at physiological temperatures, allowing cells/biomaterials to be dispensed through a syringe and deposit around the site of injury [ [53] , [54] , [55] ].…”

Section: Properties That Are Desirable For Skeletal Muscle Regeneratimentioning

confidence: 99%

Advances in biomaterials for skeletal muscle engineering and obstacles still to overcome

Smoak

Mikos

2020

Materials Today Bio

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Repair of injured skeletal muscle is a sophisticated process that uses immune, muscle, perivascular, and neural cells. In acute injury, the robust endogenous repair process can facilitate complete regeneration with little to no functional deficit. However, in severe injury, the damage is beyond the capacity for self-repair, often resulting in structural and functional deficits. Aside from the insufficiencies in muscle function, the aesthetic deficits can impact quality of life. Current clinical treatments are significantly limited in their capacity to structurally and functionally repair the damaged skeletal muscle. Therefore, alternative approaches are needed. Biomaterial therapies for skeletal muscle engineering have leveraged natural materials with sophisticated scaffold fabrication techniques to guide cell infiltration, alignment, and differentiation. Advances in biomaterials paired with a standardized and rigorous assessment of resulting tissue formation have greatly advanced the field of skeletal muscle engineering in the last several years. Herein, we discuss the current trends in biomaterials-based therapies for skeletal muscle regeneration and present the obstacles still to be overcome before clinical translation is possible. With millions of people affected by muscle trauma each year, the development of a therapy that can repair the structural and functional deficits after severe muscle injury is pivotal.

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Can we Achieve the Perfect Injectable Scaffold for Cell Therapy?

Cited by 13 publications

References 20 publications

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

Basic Principles of Emulsion Templating and Its Use as an Emerging Manufacturing Method of Tissue Engineering Scaffolds

An in vitro assessment of the response of THP‐1 macrophages to varying stiffness of a glycol‐chitosan hydrogel for vocal fold tissue engineering applications

Advances in biomaterials for skeletal muscle engineering and obstacles still to overcome

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