Can successful vaccines teach us how to induce efficient protective immune responses?

Lambert, Paul Henri; Liu, Margaret; Siegrist, Claire‐Anne

doi:10.1038/nm1216

Cited by 173 publications

(108 citation statements)

References 77 publications

(77 reference statements)

Supporting

Mentioning

102

Contrasting

Unclassified

Order By: Relevance

“…We could confirm this finding in that the neutralizing and binding titers were the highest in those individuals who were in their first decade of life during the period of time when the corresponding subtype was circulating. Older individuals also had significantly more binding and neutralizing antibodies against old than against more recent isolates (eg, H3/99; Supplementary Figure 2), which may reflect original antigenic sin [17]. In contrast, in line with other studies [18], we found antibody titers to the last prestudy isolates (2007) to be the same in all age groups.…”

Section: Discussionsupporting

confidence: 88%

Prevalence and Predictors for Homo- and Heterosubtypic Antibodies Against Influenza A Virus

Kohler¹,

Scherrer²,

Zagordi³

et al. 2014

Clinical Infectious Diseases

View full text Add to dashboard Cite

Background. The effectiveness of trivalent influenza vaccination has been confirmed in several studies. To date, it is not known whether repeated exposure and vaccination to influenza promote production of cross-reactive antibodies. Furthermore, how strains encountered earlier in life imprint the immune response is currently poorly understood.Methods. To determine the prevalence for human homo-and heterosubtypic antibody responses, we scrutinized serum samples from 305 healthy volunteers for hemagglutinin-binding and -neutralizing antibodies against several strains and subtypes of influenza A. Statistical analyses were then performed to establish the association of measured values with potential predictors.Results. It was found that vaccination not only promoted higher binding and neutralizing antibody titers to homosubtypic influenza isolates but also increased heterosubtypic human immune responses. Both binding and neutralizing antibody titers in relation with age of the donors mirrored the course of the different influenza strain circulation during the last century. Advanced age appeared to be of advantage for both binding and neutralizing titers to most subtypes. In contrast, the first virus subtype encountered was found to imprint to some degree subsequent antibody responses. Antibodies to recent strains, however, primarily seemed to be promoted by vaccination.Conclusions. We provide evidence that vaccinations stimulate both homo-and heterosubtypic immune responses in young and middle-aged as well as more senior individuals. Our analyses suggest that influenza vaccinations not only prevent infection against currently circulating strains but can also stimulate broader humoral immune responses that potentially attenuate infections with zoonotic or antigenically shifted strains.

show abstract

Section: Discussionsupporting

confidence: 88%

Prevalence and Predictors for Homo- and Heterosubtypic Antibodies Against Influenza A Virus

Kohler¹,

Scherrer²,

Zagordi³

et al. 2014

Clinical Infectious Diseases

View full text Add to dashboard Cite

show abstract

“…Despite the limitations of the immune system in early life, successful pediatric vaccines exist. Deciphering the biological basis for their efficacy will be useful for developing optimal vaccination strategies to protect the very young [54].…”

Section: Discussionmentioning

confidence: 99%

Early life T cell responses to pneumococcal conjugates increase with age and determine the polysaccharide‐specific antibody response and protective efficacy

et al. 2006

Self Cite

View full text Add to dashboard Cite

Immunization with a tetanus-protein (TT) pneumococcal polysaccharide (PPS) conjugate vaccine (Pnc1-TT) induces protective immunity against lethal pneumococcal infections in neonatal and infant mice, but anti-PPS IgG response and protective efficacy is lower than in adult mice. Here, we show that reduced antibody (Ab) response and protection against infections is directly related to impaired T cell response to the carrier. Whereas spleen cells from adult mice immunized with Pnc1-TT responded with proliferation and IFN-gamma secretion to in vitro stimulation with TT, spleen cells from neonatal and infant mice did not. However, significant, but age dependent, Th2-cytokine responses were observed in mice immunized with Pnc1-TT. Impaired IFN-gamma production upon TT-stimulation in vitro was also reflected in reduced IFN-gamma/IL-5 ratio. The IL--5 response correlated with IgG anti-PPS titers, and the lack of PPS Ab in the majority of neonatal mice was clearly associated with absence of carrier-specific IL-5 production. These results show that immunization with Pnc1-TT induces carrier-specific T cell responses that increase with age and determine the levels of PPS-specific Ab elicited. Whereas a weak and Th2-biased response was observed in neonatal mice, infant mice showed a mixed Th1-Th2 response as observed in adults

show abstract

“…However, the animals inoculated with purified PCV2 should not constitute a control group to assess the candidate vaccine, given that the imunization with purified inactivated virus is direct antigen application (Lambert et al 2005), whereas the DNA vaccination assay is an in vivo transfection assay (Babiuk et al 2003). Sera of these animals were produced only for ELISA standardization.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of a recombinant DNA vaccine candidate expressing porcine circovirus 2 structural protein

Júnior¹,

Castro²,

Chiarelli-Neto³

et al. 2009

Pesq. Vet. Bras.

View full text Add to dashboard Cite

RESUMO.-[Desenvolvimento e avaliação de um candidato à vacina de DNA recombinante expressando a proteína estrutural do circovírus suíno 2.] O circovírus suíno 2 (PCV2) é geralmente associado à síndrome da circovirose suína, que é considerada uma importante doença de suínos e possui um sério impacto econômico na suinocultura mundial. Este trabalho descreve a construção de um plasmídeo recombinante que expressa a proteína estrutural do PCV2 e a avaliação das respostas imune humoral e celular por meio de vacinação em camundongos BALB/c. O candidato vacinal foi submetido a aná-lises in vivo, determinando a capacidade de induzir resposta imune específica em camundongos. O DNA de um isolado brasileiro de PCV2 foi extraído e o gene que codifica para a proteína do capsídeo foi amplificado por PCR e inserido num plasmídeo de expressão.

show abstract

Can successful vaccines teach us how to induce efficient protective immune responses?

Cited by 173 publications

References 77 publications

Prevalence and Predictors for Homo- and Heterosubtypic Antibodies Against Influenza A Virus

Prevalence and Predictors for Homo- and Heterosubtypic Antibodies Against Influenza A Virus

Early life T cell responses to pneumococcal conjugates increase with age and determine the polysaccharide‐specific antibody response and protective efficacy

Development and evaluation of a recombinant DNA vaccine candidate expressing porcine circovirus 2 structural protein

Contact Info

Product

Resources

About