Can soluble human leucocyte antigen-G predict successful pregnancy in assisted reproductive technology?

Vercammen, Martine; Verloes, A.; Haentjens, Patrick

doi:10.1097/gco.0b013e32832924cd

Cited by 15 publications

(8 citation statements)

References 65 publications

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“…One study has indicated that the presence of sHLA-G in human embryo culture medium has been correlated with IVF outcome (89). However, there are several studies that have failed to find a relationship between the presence of sHLA-G and pregnancy, including that of Vercammen et al (269), possibly due to differences in methods of detection (270).…”

Section: Effects Of Cytokine Supplementationmentioning

confidence: 99%

Soluble Ligands and Their Receptors in Human Embryo Development and Implantation

et al. 2015

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Extensive evidence suggests that soluble ligands and their receptors mediate human preimplantation embryo development and implantation. Progress in this complex area has been ongoing since the 1980s, with an ever-increasing list of candidates. This article specifically reviews evidence of soluble ligands and their receptors in the human preimplantation stage embryo and female reproductive tract. The focus will be on candidates produced by the human preimplantation embryo and those eliciting developmental responses in vitro, as well as endometrial factors related to implantation and receptivity. Pathways to clinical translation, including innovative diagnostics and other technologies, are also highlighted, drawing from this collective evidence toward facilitating joint improvements in embryo quality and endometrial receptivity. This strategy could not only benefit clinical outcomes in reproductive medicine but also provide broader insights into the peri-implantation period of human development to improve fetal and neonatal health.

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Section: Effects Of Cytokine Supplementationmentioning

confidence: 99%

Soluble Ligands and Their Receptors in Human Embryo Development and Implantation

et al. 2015

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“…Impurity of the HLA-G standard material could result in overestimation of the sHLA-G concentration. Absolute amounts of 2-756 ng of sHLA-G have been reported in the supernatants of single or multiple embryo cultures [119], while the daily protein release of a human embryo is estimated to be 150 pg and its total protein content to be 50 ng [120]. The estimated amount of sHLA-G in embryo culture supernatants is more likely situated in the picogram range.…”

Section: Hla-g Expression In Preimplantation Embryos and Its Clinicalmentioning

confidence: 99%

The importance of HLA-G expression in embryos, trophoblast cells, and embryonic stem cells

Rizzo

Vercammen

Velde

et al. 2010

Cell. Mol. Life Sci.

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The nonclassical HLA-G molecule is a trophoblast-specific molecule present in almost every pregnancy. It differs from classical HLA class I molecules by the low degree of allelic variants and the high diversity of protein structures. HLA-G is reported to be a tolerogenic molecule that acts on cells of both innate and adaptive immunity. At the maternal-fetal interface HLA-G seems to be responsible largely for the reprogramming of local maternal immune response. This review will focus on the HLA-G gene expression profile in pregnancy, in preimplantation embryos, and in human embryonic stem cells with emphasis on the structural diversity of the HLA-G protein and its potential functional and diagnostic implications.

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“…26,28,29,31,61 (4) It influences endometrial receptivity-implantation, 52,54 pregnancy, 51,62 (5) Its aberrant levels are associated with implantation failure, 54 miscarriage 63 and adverse pregnancy outcomes, [64][65][66] (6) Its elevated levels, observed in early-late pregnancy, could be a useful parameter to monitor maternal immune response during pregnancy. 67 However, the predictive value sHLA-G as embryo viability biomarker has become controversial 53,[71][72][73] in view of contrasting reports relating to a lack of its association with successful implantation. 71 Most studies thus far have measured sHLA-G levels only in cleavage-stage embryos 33,72 and none have showed its association/correlation with pregnancy outcome in terms of live births, following transfer of morulae (day 4) or blastocysts (day 5).…”

Section: Introductionmentioning

confidence: 99%

Soluble human leukocyte antigen‐G is a potential embryo viability biomarker and a positive predictor of live‐births in humans

Vani

Vasan

Adiga

et al. 2021

American J Rep Immunol

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ProblemHuman infertility affects 15–20% of reproductive‐age couples and it is mitigated by assisted reproductive technology (ART) approaches. Poor biological viability of embryos contributes to implantation failure and live birth rate (LBR). This study is aimed to examine whether or not embryo‐secreted soluble human leukocyte antigen‐G (sHLA‐G) is (i) associated with developing embryos and (ii) able to predict successful pregnancy outcome.Method of studyA retrospective, multicentric study using 539 human embryo spent medium samples (E‐SMs), analysed for sHLA‐G levels by ELISA. Correlation analysis was performed on sHLA‐G levels with developing embryonic stages, their quality scores and pregnancy outcome in terms of LBR.ResultsOf 539 E‐SMs analysed, 445 had detectable sHLA‐G (83%) with levels varying within and across clinics and, between stages of embryonic development. Levels of sHLA‐G (ng/mL) were significantly (P < .05) different in E‐SMs of cleavage‐stage embryos versus blastocysts. There was an insignificant correlation between the sHLA‐G levels and morphology scores of embryos. But, sHLA‐G levels showed a positive correlation with grades of blastocysts and importantly, its levels were significantly (P < .05) higher in live‐birth vis‐a‐vis no‐birth cases. Also, levels were higher in live‐births out of blastocysts‐ETs versus cleavage‐stage‐embryo transfers. Altered levels were observed with embryos, which resulted in miscarriages. Overall, a significant (P < .0001) association of sHLA‐G with live births was observed.ConclusionEmbryo‐derived sHLA‐G can be a valuable embryo viability, independent, biomarker, which can predict live‐birth outcome and it could be useful as an adjunct to existing criteria for elective single embryo transfer.

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Can soluble human leucocyte antigen-G predict successful pregnancy in assisted reproductive technology?

Cited by 15 publications

References 65 publications

Soluble Ligands and Their Receptors in Human Embryo Development and Implantation

Soluble Ligands and Their Receptors in Human Embryo Development and Implantation

The importance of HLA-G expression in embryos, trophoblast cells, and embryonic stem cells

Soluble human leukocyte antigen‐G is a potential embryo viability biomarker and a positive predictor of live‐births in humans

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