Can shear stress direct stem cell fate?

Stolberg, Sarah; McCloskey, Kara E.

doi:10.1002/btpr.124

Cited by 178 publications

(116 citation statements)

References 87 publications

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“…The choice depends on the cell type and stress strength. Mild stress may improve differentiation of stem cells (Stolberg and McCloskey 2009;Hronik-Tupaj et al 2011). The outcome for unbearable stress is necrosis.…”

Section: Discussionmentioning

confidence: 99%

Sublethal heat shock induces premature senescence rather than apoptosis in human mesenchymal stem cells

Алексеенко

Zemelko

Домнина

et al. 2014

Cell Stress and Chaperones

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Stem cells in adult organism are responsible for cell turnover and tissue regeneration. The study of stem cell stress response contributes to our knowledge on the mechanisms of damaged tissue repair. Previously, we demonstrated that sublethal heat shock (HS) induced apoptosis in human embryonic stem cells. This study aimed to investigate HS response of human adult stem cells. Human mesenchymal stem cells (MSCs) cultivated in vitro were challenged with sublethal HS. It was found that sublethal HS did not affect the cell viability assessed by annexin V/propidium staining. However, MSCs subjected to severe HS exhibited features of stress-induced premature senescence (SIPS): irreversible cell cycle arrest, altered morphology, increased expression of senescence-associated β-galactosidase (SA-β-gal) activity, and induction of cyclin-dependent kinase inhibitor p21 protein. High level of Hsp70 accumulation induced by sublethal HS did not return to the basal level, at least, after 72 h of the cell recovery when most cells exhibited SIPS hallmarks. MSCs survived sublethal HS, and resumed proliferation sustained the properties of parental MSCs: diploid karyotype, replicative senescence, expression of the cell surface markers, and capacity for multilineage differentiation. Our results showed for the first time that in human MSCs, sublethal HS induced premature senescence rather than apoptosis or necrosis. MSC progeny that survived sublethal HS manifested stem cell properties of the parental cells: limited replicative life span and multilineage capacity.

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Section: Discussionmentioning

confidence: 99%

Sublethal heat shock induces premature senescence rather than apoptosis in human mesenchymal stem cells

Алексеенко

Zemelko

Домнина

et al. 2014

Cell Stress and Chaperones

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“…Other cell surface molecules, such as G-protein-coupled receptors (GPCRs) and the glycocalyx, also serve as sensors of shear stress (Ando and Yamamoto, 2009;Stolberg and McCloskey, 2009). Mechanical stress and/or ligand binding induces conformational changes in the GPCRs, which transduce a signal to the cytoplasmic tail.…”

Section: Effects Of Shear Stress On Mechanotransduction and Associatementioning

confidence: 99%

The role of mechanical stimuli in the vascular differentiation of mesenchymal stem cells

Dan

Velot

Decot

et al. 2015

Journal of Cell Science

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Mesenchymal stem cells (MSCs) are among the most promising and suitable stem cell types for vascular tissue engineering. Substantial effort has been made to differentiate MSCs towards vascular cell phenotypes, including endothelial cells and smooth muscle cells (SMCs). The microenvironment of vascular cells not only contains biochemical factors that influence differentiation, but also exerts hemodynamic forces, such as shear stress and cyclic strain. Recent evidence has shown that these forces can influence the differentiation of MSCs into endothelial cells or SMCs. In this Commentary, we present the main findings in the area with the aim of summarizing the mechanisms by which shear stress and cyclic strain induce MSC differentiation. We will also discuss the interactions between these mechanical cues and other components of the microenvironment, and highlight how these insights could be used to maintain differentiation.

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“…Units of ALP activity were calculated as described [5] and normalized to the total protein content of the sample. loading, which some have shown can infl uence stem cell differentiation [12].…”

Section: Alp Assaymentioning

confidence: 99%

Reduced Differentiation Efficiency of Murine Embryonic Stem Cells in Stirred Suspension Bioreactors

Taiani

Krawetz

Nieden

et al. 2010

Stem Cells and Development

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The use of embryonic stem cells (ESCs) for regenerative medicine has generated increased attention due to the favorable attributes of these cells; namely, they are pluripotent and possess long-term self-renewal capacity. The initial aims of the present study were: (i) to use stirred suspension bioreactors to expand and differentiate ESCs into osteogenic and chondrogenic cell types and (ii) to explore if these ESC-derived cells infl uenced skeletal healing in an in vivo fracture model. We show that differentiation protocols used in static culture are insufficient when applied directly to suspension culture bioreactors. Moreover, when bioreactor-differentiated cells are transplanted into a burr-hole defect in bone, severe disruption of the bone architecture was noted at the fracture site, as determined by microcomputed tomography (microCT) imaging and histopathology. Further characterization of the bioreactor-differentiated cultures revealed that a subpopulation of cells in the resulting aggregates expressed the pluripotency marker Oct-4 in the nucleus. Nuclear Oct-4 expression persisted even after 30 days of culture in the absence of leukemia inhibitory factor (LIF). Remarkably, and unlike ESCs differentiated into skeletal cell types in static cultures, bioreactor-differentiated aggregates implanted subcutaneously into SCID mice formed teratomas. The development of effective ESC differentiation protocols for suspension bioreactors will require a more complete understanding of the environmental conditions within these culture systems and the infl uence that these conditions have on the regulation of pluripotency and differentiation in ESCs.

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Can shear stress direct stem cell fate?

Cited by 178 publications

References 87 publications

Sublethal heat shock induces premature senescence rather than apoptosis in human mesenchymal stem cells

Sublethal heat shock induces premature senescence rather than apoptosis in human mesenchymal stem cells

The role of mechanical stimuli in the vascular differentiation of mesenchymal stem cells

Reduced Differentiation Efficiency of Murine Embryonic Stem Cells in Stirred Suspension Bioreactors

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