Can l‐ascorbic acid and trans‐resveratrol protect HaCaT cells from fine particulate matter toxicity?

Abstract

Continuous exposure of human skin to air pollution can result in a range of undesirable skin conditions. In our recent study, UV and visible light were found to increase cytotoxicity of fine particulate matter (PM2.5) against human keratinocytes. Since it is impossible to avoid exposure of human skin to PM2.5, effective strategies are needed to reduce their damaging effects. l‐ascorbic acid and resveratrol were tested as potential topical agents against pollution‐related skin impairment. Although these agents were previously found to ameliorate PM‐dependent damage, the effect of light and seasonal variation of particles were not previously studied. EPR spin‐trapping, DPPH assay, and singlet oxygen phosphorescence were used to determine the scavenging activities of the antioxidants. MTT, JC‐10 and iodometric assays were used to analyze the effect on PM2.5‐induced cytotoxicity, mitochondrial damage and oxidation of lipids. Live‐cell imaging was employed to examine wound‐healing properties of cells. Light‐induced, PM2.5‐mediated oxidative damage was examined by immunofluorescent staining. Both antioxidants effectively scavenged free radicals and singlet oxygen produced by PM2.5, reduced cell death and prevented oxidative damage to HaCaT cells. l‐ascorbic acid and resveratrol, especially when applied in combination, can protect HaCaT cells against the dark and light induced toxicity of PM2.5.

Polyphenols: Natural Food-Grade Biomolecules for the Treatment of Nervous System Diseases from a Multi-Target Perspective

“…Trans-resveratrol (TRE), a naturally occurring polyphenol found in high concentrations in grape seeds and skins, has been the subject of extensive research because of its potent antioxidant, anti-inflammatory, and antipsychotic characteristics [115]. Studies have shown that TRE can effectively scavenge PM-generated free radicals and mono-linear oxygen 2.5, reduce cell death and prevent oxidative damage to HaCaT cells, and protect HaCaT cells against the dark and light induced toxicity of PM2.5 [116].…”

NMR spectral data - notable testimony in antioxidant interactions research: case studies of some grape metabolites

“…Cele mai recente date de literatură susțin rezultatele descrise de noi. Astfel, Mokrzyński și colaboratorii au folosit testul DPPH ca parte a unei abordări complexe dedicate evaluării impactului AA și tRes asupra leziunii oxidative mediate de materia particulată (PM 2.5 ) asupra celulelor HaCaT (linia celulară de keratinocite epidermice umane), ca model de epidermă [24]. A fost descoperit, că atât AA, cât și tRes, dar mai ales atunci când sunt aplicați în combinație, pot proteja celulele HaCaT împotriva toxicității UV și a luminii vizibile induse de PM 2.5 prin eliminarea eficientă a radicalilor liberi și a oxigenului singlet produs de PM 2.5 .…”