Can ruminal inoculum from slaughtered cattle replace inoculum from cannulated cattle for feed evaluation research?

Alba, Henry Daniel Ruiz; Oliveira, Ronaldo Lopes; Carvalho, Silvana Teixeira; Ítavo, Luís Carlos Vinhas; Ribeiro, Ossival Lolato; Júnior, Nilton Guedes do Nascimento; Freitas, Marcondes Dias; Bezerra, Leílson Rocha

doi:10.5433/1679-0359.2018v39n5p2133

Cited by 5 publications

(8 citation statements)

References 18 publications

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“…To avoid sources of variation that affect the ruminal inoculum, sampling should be similar in time and collection method as described by Fortina et al [21]. Using univariate analysis methods, Alba et al [20] concluded that the rumen inoculum of cattle in slaughterhouses with unknown data, such as feeding, management, sex, breed, and others, can be used as a potential substitute for rumen inoculum obtained from cannulated cattle. However, the authors found some differences, as did other authors who used other sources of ruminal inoculum [36].…”

Section: Discussionmentioning

confidence: 99%

“…Twenty-six TNT bags (24 with a sample and 2 blank) were placed into jars (totaling 8 jars, previously heated to 40 • C, with the continuous addition of CO 2 ), totaling 104 bags/machine. Immediately, 1600 mL of buffer solution and 400 mL of ruminal inoculum were added [20]. The incubation process was carried out at 39 • C for 48 h. Subsequently, 40 mL of HCl (6N) and 8 g of pepsin (Sigma 1:10,000; dissolved in 34 mL of distilled H 2 O) were added and incubated for an additional 24 h. Afterwards, the bags were washed with tap water until the water ran clean, pre-dried in forced-air ovens (55 • C for 12 h), dried at 105 • C for 16 h, and weighed.…”

Section: Experimental Design Diets and In Vitro Analysismentioning

confidence: 99%

“…To mimic the rumen environment, the in vitro method uses ruminal inoculum obtained from cannulated animals. However, the use of cannulated animals has been criticized due to practical problems, ethical reasons, animal welfare, and the long-term maintenance costs [18,20]. Therefore, the search for alternative inoculum is essential to reduce the use of cannulated animals.…”

Section: Introductionmentioning

confidence: 99%

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Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate Approach

Alba,

Lima,

Carvalho

et al. 2023

Ruminants

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The objective of the current study was to compare the rumen inoculum of slaughtered cattle with the ruminal inoculum of cannulated cattle; additionally, determine its reliability in the in vitro analysis of ruminant feeds throughout a multivariate approach. Five male bovines (weight 320 ± 9.4 kg; mean ± standard deviation) provided with ruminal cannula and between five and seven bovines slaughtered in slaughterhouse were used. The evaluations were carried out following a completely randomized design. The data obtained were subjected to different multivariate analyzes to determine the reliability of the ruminal inoculum of animals slaughtered in commercial slaughterhouses compared to that obtained from cannulated animals. The relative contribution indicated that the in vitro dry matter digestibility (IVDMD, 50.75%) and in vitro neutral detergent fiber digestibility (IVNDFD, 29.83%) analysis influence 80.13% of the results to determine the reliability of the ruminal inoculum from slaughtered cattle. Furthermore, it was determined that the first two principal components (IVNDFD and acetic acid production) are the ones that influence the results by 89.87%. The grouping of diets using the Tocher optimization method and the dendrogram shows the formation of six groups and two groups, respectively. The grouping shows that the ruminal inoculum source was not the limiting parameter in the evaluation. Rumen inoculum from cattle slaughtered in a commercial slaughterhouse (with unknown diet) has potential as an alternative for the in vitro analysis of cattle feed, provided that the lignin concentration in the diet is less than 35.5 g/kg DM.

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Section: Discussionmentioning

confidence: 99%

Section: Experimental Design Diets and In Vitro Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate Approach

Alba,

Lima,

Carvalho

et al. 2023

Ruminants

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“…In conclusion, the ruminal inoculum obtained from slaughtered cattle is a viable alternative to using fistulated animals for digestibility analysis. This approach is ethically more humane as it eliminates the need for invasive procedures, thus reducing animal suffering [26] .…”

Section: Substituting Slaughtered Cattle Ruminal Inoculum For Cannula...mentioning

confidence: 99%

Utilization of ruminal content from slaughtered animals: A review

Ram,

Mandal,

Kasthuri

2024

Int. J. Agric. Extension Social Dev.

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Rumen content is abundantly available at slaughterhouses. It is seen as a slaughter waste creating environmental pollution. Animal feed accounts for up to 70% of the overall cost of production. So feeding is typically the main factor restricting the production of cattle in underdeveloped nations. In recent years, there has been a significant surge in the utilization of ruminal content for creating various formulations and presentations in animal feed production. This includes both direct incorporation of ruminal content and its processing to generate commercial products. The rumen content is one of the uncommon components and is easily accessible at slaughterhouses. Furthermore, it is a good source of protein and energy (feed and microbial protein), vitamins and minerals, particularly vitamin B complex. The nutritional value of rumen contents differ mostly due to the radically varied compositions of rumen contents from various species of ruminants. There are numerous ways to increase the nutritional value of rumen content viz, incorporating blood meal, poultry manure, molasses, urea, enzymes, and fermented feeds like silage. The composition of rumen material may be based on the diet of animals. It is an affordable source of protein and energy while lowering feed costs and pollution. Some researchers have also concluded that animals' feed might include rumen contents without having a deleterious impact on diet that is acceptable and has no negative consequences on performance. The main object of this review article is to draw attention towards the nutritional value of ruminal content and its potential as an animal feed. Here in this review article, we have discussed about various methods for utilization of slaughter house origin ruminal content viz, ensiling of ruminal content, Ruminal content has found a diverse range of applications in various sectors. It is employed as a bio activator for fermenting goat manure to produce fertilizer. Additionally, spray-dried rumen fluid (SDR) serves as a valuable feed additive. Sun-dried bovine rumen content (SDRC) is utilized as an ingredient in rations for white leghorn layers. Furthermore, ruminal content contributes to biogas generation and bioenergy production. It also serves as a fertilizer for soybean and corn crops. In research settings, it can replace inoculum from cannulated cattle for feed evaluation. Moreover, ruminal content is employed as an organic fertilizer for pot cultivation of vegetables and serves as animal feed for various species including poultry, fish, rabbits, quail, swine, sheep, and ducks.

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“…A more ethically acceptable approach that reduces stress and alleviates the suffering of animals by avoiding an invasive procedure is the collection of RF at slaughtering. The RF collected at slaughter in controlled conditions has a limited difference from that sampled by other methods [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials

Fortina

Patrucco

Barbera

et al. 2022

MPs

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Digestibility trials need a viable rumen fluid as inoculum to degrade feeds. The variability of rumen fluid depends on the animal’s diet, while its viability is greatly influenced by the sampling and handling procedures. In this article, we present a replicable protocol for sampling the rumen fluid from slaughtered animals for in vitro digestibility trials. A detailed list of the tools and a step-by-step standardized procedure for the collection, storage and the transportation of the rumen fluid from the slaughterhouse to the laboratory is presented. We also describe a digestibility trial for establishing the maximum storage time of rumen fluid from sampling to its use. The results show that the rumen fluid, collected and maintained according to the proposed protocol, can be stored and used from 30 to 300 min from sampling without significantly compromising the fermentative activity of the microbial population.

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Can ruminal inoculum from slaughtered cattle replace inoculum from cannulated cattle for feed evaluation research?

Cited by 5 publications

References 18 publications

Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate Approach

Rumen Content from Slaughterhouse as an Alternative Inoculum Source for In Vitro Analysis of Feeds: A Multivariate Approach

Utilization of ruminal content from slaughtered animals: A review

Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials

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