Can Platelet rich plasma stimulate human ACL growth in culture? A preliminary experience

Dhillon, Mandeep Singh; Karna, Saroj Kumar; Dhatt, Sarvdeep Singh; Behera, Prateek; Bhatia, Anju

doi:10.11138/mltj/2015.5.3.156

Cited by 12 publications

(10 citation statements)

References 24 publications

(16 reference statements)

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“…Over the past several years, there has been increasing interest in biologic agents as both nonoperative treatment modalities and augments to surgical procedures, to potentially accelerate the healing process and expedite return to sport after muscle injury. Platelet-rich plasma (PRP) is a blood product that is rich in platelets and many different cytokines and growth factors, such as transforming growth factor beta (TGF-β1), insulin-like growth factor-1 (IGF-1), and vascular endothelial growth factor (VEGF), whose influence on the healing of ligaments, muscles, tendons, joints, and soft tissue has been extensively studied[1-5]. In muscle pathology in particular, PRP’s abundance of growth factors and cytokines is purported to expedite healing through the induction of cellular proliferation and migration, increased angiogenesis, and muscle tissue regeneration[6-8].…”

Section: Introductionmentioning

confidence: 99%

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Kunze¹,

Hannon²,

Fialkoff³

et al. 2019

WJO

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BACKGROUND Platelet-rich plasma (PRP) is an increasingly used biologic adjunct for muscle injuries, as it is thought to expedite healing. Despite its widespread use, little is known regarding the mechanisms by which PRP produces its efficacious effects in some patients. AIM To clarify the effects of PRP on muscular pathologies at the cellular and tissue levels by evaluating the basic science literature. METHODS A systematic review of PubMed/MEDLINE and EMBASE databases was performed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and checklist. Level III in vivo and in vitro studies examining PRP effects on muscles, myocytes and/or myoblasts were eligible for inclusion. Extracted data included PRP preparation methods and study results. RESULTS Twenty-three studies were included (15 in vivo , 6 in vitro , 2 in vitro / in vivo ). Only one reported a complete PRP cytology (platelets, and red and white blood cell counts). Five in vitro studies reported increased cellular proliferation, four reported increased gene expression, and three reported increased cellular differentiation. Five in vivo studies reported increased gene expression, three reported superior muscle regeneration, and seven reported improved histological quality of muscular tissue. CONCLUSION The basic science literature on the use of PRP in muscle pathology demonstrates that PRP treatment confers several potentially beneficial effects on healing in comparison to controls. Future research is needed to determine optimal cytology, dosing, timing, and delivery methods of PRP for muscle pathologies.

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Section: Introductionmentioning

confidence: 99%

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Kunze¹,

Hannon²,

Fialkoff³

et al. 2019

WJO

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“…However, the improvement was much better in the PRP group than in the corticosteroid group with better foot scores and lower pain scores. Stimulation of human ACL growth in culture by PRP in an in vitro study 14 : Analysis of cultured cells showed that addition of PRP (5 or 10%) increased the viability of ACL cells in 4 out of 11 and promoted cell proliferation in 8 of 11 donor samples; 10% PRP was more effective than 5% PRP. However, the difference in effectiveness of 10% PRP was not significantly better than 5% PRP; 5% PPP had no significant effect on cell viability, but it led to an increase in the DNA content in 5 of 11.…”

Section: Resultsmentioning

confidence: 99%

“…3. Stimulation of human ACL growth in culture by PRP in an in vitro study 14 : A controlled laboratory study was designed and ACL remnants were collected from the tibial stump of torn ACL with a punch from 28 patients during arthroscopic ACL reconstruction. Successful primary culture was obtained in 11 out of 28 samples of ACL remnant tissue; cells were isolated, identified, and cultured and were then divided into six groups: a.…”

Section: Methodology Of Six Studies Done At Pgimermentioning

confidence: 99%

Platelet-rich Plasma in Orthopedics: Efficacy, Evidence, and Evolution of Our Understanding over 10 Years

2018

Journal of Postgraduate Medicine, Education and Research

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Introduction: Platelet-rich plasma (PRP) is fast emerging as a minimally invasive treatment option in a wide variety of orthopedic conditions. We were the forerunners in India in its use in osteoarthritis (OA), lateral epicondylitis, plantar fasciitis, and sports injuries. We present our experience with prospective clinical studies and multiple research projects, and have shown some aspects to be reproducible and evidence-based, whereas some areas of application seem to demand more evidence prior to routine recommendation for use.

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“…Dhillon et al [28] evaluated the effect of human ACL cell growth of 11 patients during ACL reconstruction surgery. The ACL remnants were collected, and the cells were isolated, identified and cultured and then divided into six groups.…”

Section: Use Of Platelet-rich Plasma In Muscle and Ligament Injuriesmentioning

confidence: 99%

Use of platelet-rich plasma in orthopaedics and traumatology

Niewczas¹,

Kotela²,

Łęgosz³

et al. 2016

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Platelet rich plasma (PRP) is a concentration of blood platelets suspended in a small volume of plasma, and it can be obtained by centrifuging the patient's whole blood. It is used in many fields of medicine, including orthopaedics and traumatology. The range of indications for PRP therapy has been extended. The mechanism of action of PRP is based on function of growth factors. The application of PRP can be considered for tendinopathy and enthesopathy treatment, osteoarthritis treatment, muscle and ligament injuries, bone lesion treatment, bone infections and many other diseases. There are no standardised guidelines concerning PRP therapy, and the most efficient and optimal method of PRP preparation is yet to be determined. This article aims to present the opportunities of PRP therapy in orthopaedics and traumatology and evaluate its effectiveness. StreszczenieOsocze bogatopłytkowe (PRP) to koncentrat płytek krwi w niewielkiej ilości osocza. Uzyskiwane jest ono z krwi pełnej pacjenta przez jej odwirowanie. Stosowane jest w wielu gałęziach medycyny, m.in. ortopedii i traumatologii narządu ruchu. Zakres wskazań do stosowania terapii osoczem bogatopłytkowym ciągle się rozszerza. Mechanizm działania osocza bogatopłytkowego opiera się na działaniu płytkowych czynników wzrostu. Leczenie osoczem bogatopłytkowym może być zastosowane w tendinopatiach i entezopatiach, chorobie zwyrodnieniowej stawów, w leczeniu urazów mięśni i więzadeł, w uszkodzeniach kości, w zakażeniach narządu ruchu oraz wielu innych jednostkach chorobowych. Nie udało się dotąd stworzyć wystandaryzowanych zaleceń dotyczących stosowania osocza bogatopłytkowego. Brakuje również najkorzystniejszej i najbardziej optymalnej metody pozyskiwania koncentratu krwinek płytkowych. Celem artykułu jest zaprezentowanie możliwości terapii osoczem bogatopłytkowym w ortopedii i traumatologii narządu ruchu.

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Can Platelet rich plasma stimulate human ACL growth in culture? A preliminary experience

Cited by 12 publications

References 24 publications

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

Platelet-rich Plasma in Orthopedics: Efficacy, Evidence, and Evolution of Our Understanding over 10 Years

Use of platelet-rich plasma in orthopaedics and traumatology

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