Can Platelet-rich Plasma Be Used for Skin Rejuvenation? Evaluation of Effects of Platelet-rich Plasma on Human Dermal Fibroblast

Abstract

BackgroundAutologous platelet-rich plasma has attracted attention in various medical fields recently, including orthopedic, plastic, and dental surgeries and dermatology for its wound healing ability. Further, it has been used clinically in mesotherapy for skin rejuvenation.ObjectiveIn this study, the effects of activated platelet-rich plasma (aPRP) and activated platelet-poor plasma (aPPP) have been investigated on the remodelling of the extracellular matrix, a process that requires activation of dermal fibroblasts, which is essential for rejuvenation of aged skin.MethodsPlatelet-rich plasma (PRP) and platelet-poor plasma (PPP) were prepared using a double-spin method and then activated with thrombin and calcium chloride. The proliferative effects of aPRP and aPPP were measured by [3H]thymidine incorporation assay, and their effects on matrix protein synthesis were assessed by quantifying levels of procollagen type I carboxy-terminal peptide (PIP) by enzyme-linked immunosorbent assay (ELISA). The production of collagen and matrix metalloproteinases (MMP) was studied by Western blotting and reverse transcriptase-polymerase chain reaction.ResultsPlatelet numbers in PRP increased to 9.4-fold over baseline values. aPRP and aPPP both stimulated cell proliferation, with peak proliferation occurring in cells grown in 5% aPRP. Levels of PIP were highest in cells grown in the presence of 5% aPRP. Additionally, aPRP and aPPP increased the expression of type I collagen, MMP-1 protein, and mRNA in human dermal fibroblasts.ConclusionaPRP and aPPP promote tissue remodelling in aged skin and may be used as adjuvant treatment to lasers for skin rejuvenation in cosmetic dermatology.

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

“…On the other hand, EGF and IGF-1 also stimulate proliferation [71,72] but less potently than PDGF or bFGF. Furthermore, consistent with our results, a recent work [73] reports that PRP markedly increases fibroblast proliferation compared with the serum-treated control group. Also in HUVEC, the CGF supplementation positively influences cell growth, but less so than in fibroblasts.…”

Is there any association between mycosis fungoides and Th-1, Th-2 cytokines and transforming growth factor-beta 1 gene polymorphisms?

“…In the literature, a number of studies state that PRP forms fibroblast proliferation in tendon, dermis, gingiva, and synovium. The results of this study showed an increase in fibroblast proliferation similar to that found in previous studies 36 . The purpose of the epidermis at the stage of reepithelization after skin injury is to provide full layer epidermis formation.…”

“…Density increase was high in the PRP group compared to the PPP group but the difference was significant 34,35 . In a study conducted by Kim et al 36 , collagen and fibroblast activity was measured by incubating PRP and PPP in fibroblast culture obtained from foreskin. According to the results, it was concluded that both PRP and PPP increased type 1 collagen and fibroblast activity.…”

Effect of autologous platelet-rich plasma in the promotion of healing of chronic ulcers

“…This may provide an explanation of wound healing effect of PRP. 18 Moreover PRP which was activated by 5% calcium chloride PRP stimulates the proliferation of human dermal fibroblast. 19 Clinical studies regarding the role of PRP in chronic wound healing are increasing in number.…”