Can plant phenolic compounds reduceFusariumgrowth and mycotoxin production in cereals?

Abstract

To assess the in vitro activity of three phenolic acids (ferulic acid, p-hydroxybenzoic acid, vanillic acid) and two flavonols (quercetin, rutin) on mycelial growth and mycotoxin accumulation of Fusarium graminearum (FG), F. langsethiae (FL) and F. poae (FP), two different approaches were chosen. First, grains from oat varieties were inoculated with a suspension of three FL isolates to determine the influence of phenolic compounds on the accumulation of mycotoxins. The oat variety Zorro showed a tendency for lower accumulation of T-2/HT-2, diacetoxyscirpenol and neosolaniol. Second, a mycelium growth assay was conducted to follow FG, FL and FP growth on cereal based media supplemented with phenolic compounds. Increasing concentrations of ferulic acid substantially inhibited growth of FG and FL, while FP growth was reduced to 57%. In contrast, p-hydroxybenzoic acid, vanillic acid, quercetin, and rutin slightly stimulated mycelium growth. Results about mycotoxin production in cereal based media were less conclusive.

Coating seeds with Trichoderma strains promotes plant growth and enhance the systemic resistance against Fusarium crown rot in durum wheat

“…This suggests that phenolics most likely played a key role in Table 4 Results of one-factor ANOVA for disease incidence and disease severity on plants inoculated with F. culmorum. The F values are shown (*p < 0.05; **p < 0.01; ***p < 0.001) the observed resistance which has been reported to be involved in the cell wall reinforcement and may supply structural resistance to invading pathogens (Schöneberg et al 2018). This is in line with the findings of Lorenc-Kukuła et al (2007) who demonstrated that the increase in resistance of transgenic flax (Linum usitatissimum) seedlings to F. culmorum was mediated by three key enzymes of flavonoid biosynthesis.…”

Control of Fusarium graminearum in Wheat With Mustard-Based Botanicals: From in vitro to in planta

“…Ponts et al (2011) showed that ferulic, coumaric, caffeic, syringic, and p-hydroxybenzoic acids inhibited the radial growth of four F. graminearum strains by 50%. Schöneberg et al (2018a) demonstrated that ferulic acid substantially inhibited the mycelium growth of F. graminearum, F. langsethiae and F. poae, while p-hydroxybenzoic and vanillic acids had no effect.…”

Investigating the Efficiency of Hydroxycinnamic Acids to Inhibit the Production of Enniatins by Fusarium avenaceum and Modulate the Expression of Enniatins Biosynthetic Genes

“…Ferruz et al [20] have shown that the growth of F. langsethiae was enhanced with 0.5 mM of FER while 1 mM of FER led to a reduction of the fungal biomass. Likewise, Schöneberg et al [21] have reported that the growth of F. graminearum and F. poae was increased by exposure to p-hydroxybenzoic acid. According to Kulik et al [19], some phenolic acids could trigger a stimulation of the biosynthesis of ergosterol in F. graminearum and F. culmorum.…”

“…Prevention measures based on the use of phytochemicals with antimicrobial activities have been investigated [16]. Increasingly, plant phenolic compounds have become the subject of mycotoxin research and many groups have demonstrated their capacity to restrain the fungal growth and the production of various mycotoxins, including Fusarium mycotoxins, such as deoxynivalenol [17][18][19], T2 and HT2 toxins [20,21], and fumonisins [22,23]. However, to the best of our knowledge, no study has examined the effect of phenolic compounds on the growth of ENNs-producing Fusarium species and on ENN biosynthesis.…”