Can pigment gallstones be induced by biliary stricture and prevented by medicine in Guinea pigs

Abstract

AIM:To determine the relationship between biliary stricture and pigment gallstone formation, and the prevention of pigment gallstones with medicine. METHODS:One hundred and eighteen male guinea pigs were randomly divided into four groups: stricture group (S, n = 30) underwent partial ligation of common bile duct, and fed on regular chow; S plus medicine group (S+M, n = 27) underwent the same operation but fed on medicinal chow (0.3 g chenodeoxycholic acid, 0.5 g glucurolactone, and 0.5 g aspirin were mixed up in 1.2 kg regular chow); medicinal control group (C+M, n = 30) was free of operation, and fed on medicinal chow; and control group (C, n = 31) was free of operation and fed on regular chow. One week later, laparotomy was performed, and the bile of gallbladder was collected, measured, and cultured. RESULTS:Gallstones were identified. Pigment gallstones were induced by biliary stricture in 95% (22/23) of S group. In the S+M group, the incidence of gallstone was reduced to 55% (11/20, vs S group, P < 0.01). The changes of indirect bilirubin and ionized calcium in the bile were consistent with gallstone incidences. CONCLUSION:Biliary stricture can cause pigment gallstone formation in guinea pigs, and the medicines used can lower the incidence of gallstones. The bilirubin and ionized calcium play important roles in pigment gallstone formation.

Development of an animal model for assessment of primary end-to-end biliary reconstruction

“…Additionally, gallstones are found in 6% (3/50) of rabbits. Stones have formed within 2 months postoperatively, which is similar to results from our previous experiment that biliary stricture can cause pigment gallstone formation in guinea pigs [25]. These results suggest that anastomotic biliary stricture may cause the absence of material for bile acids synthesis and it may play an important role in the formation of gallstone.…”

The Impact of Thyroid Dysfunction and TSH on the Pathogenesis of Gallstone and its Composition

“…Table (2) reveals that high serum TSH levels were more often of male gender , while females were demonstrated high prevalence of normal TSH. Table (4) shows that age was studied to be effect on the predominant cholesterol gallstone with old age patients. Table (5) reveals The relation between gallstone type and sex that was found a significant and cholesterol gallstone in females patients has a high rate.…”

“…After the morphological study, gallstones were washed, dried and grinned to a powder. Powder was weighed and was taken for chemical analysis using conversional methods for chemical analysis (4) .TSH level was determined (5) . In the analysis of serum TSH, patients classified by the results obtained during analysis for serum TSH among 100 patients 10 with low TSH, 52 who had high TSH and 38 who had normal serum TSH.…”

Healing Process of the Guinea Pig Common Bile Duct after End-to-End Anastomosis: Pathological Evaluation after 6 Months

“…Moreover, though dogs might be better candidates for biliary reconstruction, from an animal welfare and ethical point of view, guinea pigs are better suited and cheaper and therefore we chose guinea pigs in this preliminary experiment. Also, the guinea pig is a frequently used animal model in studies of the biliary system, especially when investigating gallstone formation [17,18,19]. …”