Can ornithine accumulation modulate abiotic stress tolerance in Arabidopsis?

Kalamaki, Mary S.; Merkouropoulos, Georgios; Kanellis, Angelos K.

doi:10.4161/psb.4.11.9873

Cited by 87 publications

(62 citation statements)

References 19 publications

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“…This goes hand in hand with the observed increase in 2-oxogluterate, which is produced via GGT action induced after shifting C. reinhardtii cells into low CO 2 conditions (112), whereas glutamate is decreased because it is steadily consumed. Notably arginine accumulates under stress in A. thaliana (118,119) and is considered an important intermediate for nitrogen storage (120). In line our data suggest that arginine biosynthesis, as well as photorespiration, is characteristic for cells actively performing photosynthesis.…”

Section: Remodeling Of Bioenergetics Pathways Under Iron-deficiency Isupporting

confidence: 83%

The Metabolic Status Drives Acclimation of Iron Deficiency Responses in Chlamydomonas reinhardtii as Revealed by Proteomics Based Hierarchical Clustering and Reverse Genetics

Höhner

Barth

Magneschi

et al. 2013

Molecular & Cellular Proteomics

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Iron is a crucial cofactor in numerous redox-active proteins operating in bioenergetic pathways including respiration and photosynthesis. Cellular iron management is essential to sustain sufficient energy production and minimize oxidative stress. To produce energy for cell growth, the green alga Chlamydomonas reinhardtii possesses the metabolic flexibility to use light and/or carbon sources such as acetate. To investigate the interplay between the iron-deficiency response and growth requirements under distinct trophic conditions, we took a quantitative proteomics approach coupled to innovative hierarchical clustering using different "distance-linkage combinations" and random noise injection. Protein co-expression analyses of the combined data sets revealed insights into cellular responses governing acclimation to iron deprivation and regulation associated with photosynthesis dependent growth. Photoautotrophic growth requirements as well as the iron deficiency induced specific metabolic enzymes and stress related proteins, and yet differences in the set of induced enzymes, proteases, and redoxrelated polypeptides were evident, implying the establishment of distinct response networks under the different conditions. Moreover, our data clearly support the notion that the iron deficiency response includes a hierarchy for iron allocation within organelles in C. reinhardtii. Importantly, deletion of a bifunctional alcohol and acetaldehyde dehydrogenase (ADH1), which is induced under low iron based on the proteomic data, attenuates the remodeling of the photosynthetic machinery in response to iron deficiency, and at the same time stimulates expression of stress-related proteins such as NDA2, LHCSR3, and PGRL1. This finding provides evidence that the coordinated regulation of bioenergetics pathways and iron deficiency response is sensitive to the cellular and chloroplast metabolic and/or redox status, consistent with systems approach data. Molecular &

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Section: Remodeling Of Bioenergetics Pathways Under Iron-deficiency Isupporting

confidence: 83%

The Metabolic Status Drives Acclimation of Iron Deficiency Responses in Chlamydomonas reinhardtii as Revealed by Proteomics Based Hierarchical Clustering and Reverse Genetics

Höhner

Barth

Magneschi

et al. 2013

Molecular & Cellular Proteomics

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“…Although there was no significant difference in proline content between drought and control conditions, ornithine accumulated significantly in both poplar species. In SlNAGS1 ( Solanum lycopersicum N‐acetyl‐L‐glutamate synthase gene)‐overexpressing plants, ornithine accumulation is coupled to the production of osmoprotectants that enhance stress tolerance (Kalamaki et al ). The observed accumulation of ornithine in drought‐stressed poplar plants suggests that ornithine might be closely associated with plant drought tolerance.…”

Section: Discussionmentioning

confidence: 99%

Comparative metabolomics analysis reveals different metabolic responses to drought in tolerant and susceptible poplar species

Jia

Wang

et al. 2019

Physiologia Plantarum

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Drought is one of the critical factors limiting tree growth and survival. Clarifying the adaptation to drought will facilitate the cultivation of drought‐tolerant varieties. Metabolites, as direct signatures of biochemical functions, can uncover the biochemical pathways involved in drought responses. Here, we investigated the physiological and metabolic responses of drought‐tolerant Populus simonii and drought‐susceptible Populus deltoides cv. ‘Danhong’ to drought. Under drought conditions, P. simonii grew better and had a higher photosynthetic rate than P. deltoides cv. ‘Danhong’. Global untargeted metabolite profiling was analyzed using gas chromatography time‐of‐flight mass spectrometry system. A total of 69 and 53 differentially accumulated metabolites were identified in drought‐stressed P. simonii and P. deltoides cv. ‘Danhong’, respectively. The metabolisms of carbohydrate, amino acid, lipid and energy were involved in the drought responses common to both poplar species. The citric acid cycle was significantly inhibited to conserve energy, whereas multiple carbohydrates acting as osmolytes and osmoprotectants were induced to alleviate the adverse effects of drought stress. Unlike P. deltoides cv. ‘Danhong’, P. simonii underwent a specific metabolic reprogramming that enhanced non‐enzymatic antioxidants, coordinated the cellular carbon/nitrogen balance and regulated wax biosynthesis. These results provide a reference for characterizing the mechanisms involved in poplar response to drought and for enhancing the drought tolerance of forest trees.

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“…Genetically engineered plants provide evidence that enhanced trehalose metabolism can positively regulate stress tolerance (Avonce et al, 2004;Suzuki et al, 2008;Li et al, 2011). Orn is the intermediate compound in the Arg biosynthetic pathway, where the pathway diverges to the production of compounds, such as Pro and polyamines, which serve osmoprotective functions and improve stress tolerance (Kalamaki et al, 2009). Glycinebetaine is synthesized either by oxidation of choline or by N-methylation of Gly (Chen and Murata, 2002).…”

Section: Condition-specific Modulators and Sustainersmentioning

confidence: 99%

Integration of Genome-Scale Modeling and Transcript Profiling Reveals Metabolic Pathways Underlying Light and Temperature Acclimation in Arabidopsis

et al. 2013

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Understanding metabolic acclimation of plants to challenging environmental conditions is essential for dissecting the role of metabolic pathways in growth and survival. As stresses involve simultaneous physiological alterations across all levels of cellular organization, a comprehensive characterization of the role of metabolic pathways in acclimation necessitates integration of genome-scale models with high-throughput data. Here, we present an integrative optimization-based approach, which, by coupling a plant metabolic network model and transcriptomics data, can predict the metabolic pathways affected in a single, carefully controlled experiment. Moreover, we propose three optimization-based indices that characterize different aspects of metabolic pathway behavior in the context of the entire metabolic network. We demonstrate that the proposed approach and indices facilitate quantitative comparisons and characterization of the plant metabolic response under eight different light and/or temperature conditions. The predictions of the metabolic functions involved in metabolic acclimation of Arabidopsis thaliana to the changing conditions are in line with experimental evidence and result in a hypothesis about the role of homocysteine-to-Cys interconversion and Asn biosynthesis. The approach can also be used to reveal the role of particular metabolic pathways in other scenarios, while taking into consideration the entirety of characterized plant metabolism.

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Can ornithine accumulation modulate abiotic stress tolerance in Arabidopsis?

Cited by 87 publications

References 19 publications

The Metabolic Status Drives Acclimation of Iron Deficiency Responses in Chlamydomonas reinhardtii as Revealed by Proteomics Based Hierarchical Clustering and Reverse Genetics

The Metabolic Status Drives Acclimation of Iron Deficiency Responses in Chlamydomonas reinhardtii as Revealed by Proteomics Based Hierarchical Clustering and Reverse Genetics

Comparative metabolomics analysis reveals different metabolic responses to drought in tolerant and susceptible poplar species

Integration of Genome-Scale Modeling and Transcript Profiling Reveals Metabolic Pathways Underlying Light and Temperature Acclimation in Arabidopsis

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