Can morphological features evaluated in oral cancer biopsies influence in decision-making? A preliminary study

The Emerging Impact of Tumor Budding in Oral Squamous Cell Carcinoma: Main Issues and Clinical Relevance of a New Prognostic Marker

“…Single cut-off: 3 buds/field [ 31 , 32 , 44 , 64 ] 4 buds/field [ 55 ] 5 buds/fields [ 16 , 23 , 24 , 26 , 29 , 33 , 34 , 37 , 38 , 40 , 43 , 53 , 58 , 59 , 60 , 63 , 64 , 67 , 69 , 71 ] 10 buds/field [ 15 , 19 , 61 , 62 ] 15 buds/field [ 24 ] …”

“…5 buds/fields [ 16 , 23 , 24 , 26 , 29 , 33 , 34 , 37 , 38 , 40 , 43 , 53 , 58 , 59 , 60 , 63 , 64 , 67 , 69 , 71 ]…”

“…n. of HPF evaluated: 1, 5, 10 1 [ 5 , 15 , 16 , 18 , 19 , 23 , 24 , 25 , 26 , 28 , 29 , 30 , 32 , 34 , 36 , 37 , 38 , 40 , 43 , 44 , 53 , 54 , 55 , 56 , 57 , 60 , 61 , 62 , 63 , 64 , 65 , 67 , 68 , 69 , 71 , 73 , 74 ] 5 [ 15 , 33 ] 10 [ 19 , 24 , 66 …”

Oral cancer: changing the aim of the biopsy in the age of precision medicine. A review

“…Inside TME cancer cells, non-tumour cells and stroma are in a continuous and dynamic relationship, and any change within the TME may reflect changes of the balance between the immune system and tumour 63 . In this context, the role of biopsy is not only diagnostic, but also predictive, since biopsies should include the invasive front, the behaviour of tumour cells in the TME interface and biomarkers that provide useful information to guide the therapeutic choice 15,64,[65][66][67] . Leite et al 66 , in a recent preliminary study on biopsy samples from 56 OSCC cases, highlighted the possible importance of the correlation between grading, predominant mode of invasion and TB intensity.…”

“…In this context, the role of biopsy is not only diagnostic, but also predictive, since biopsies should include the invasive front, the behaviour of tumour cells in the TME interface and biomarkers that provide useful information to guide the therapeutic choice 15,64,[65][66][67] . Leite et al 66 , in a recent preliminary study on biopsy samples from 56 OSCC cases, highlighted the possible importance of the correlation between grading, predominant mode of invasion and TB intensity. The authors classified the mode of invasion in 4 degrees, similar to the proposal of Shimuzu 54 ; they also graded TB as low-intensity (5 buds in one single x200 power field) and high-intensity (≥ 5 buds in Almangush, 2018 58 BD Model Score (BDM): N° of buds and depth of invasive front (IF, mm) 0: < 5 buds at the IF and depth < 4 mm 1: =/> 5 buds at the IF or depth > 4 mm 2: =/> 5 buds at the IF and depth > 4mm Seki, 2017 48 Tumour Budding Score ( 61 Risk Score: Sum of single score of clinical and pathologic parameters Shape of rete pegs: Slender and fused = 1, Bulbous and uniform = 2; Irregular = 3 Pattern of invasion: Pushing = 1; Minimally invasive = 2; Frankly invasive = 5 Depth of invasion (TT, mm): 0-3 = 1; > 3 = 3 Histogic differentiation: Well = 1; Moderate = 3; Poor = 5 Site of cancer: Buccal mucosa = 0; Tongue = 1; Others = 2 one single x200 power field).…”