Can MDM2 and CDK4 make the diagnosis of well differentiated/dedifferentiated liposarcoma? An immunohistochemical study on 129 soft tissue tumours

Aleixo, Pedro Bandeira; Hartmann, Arndt; Menezes, Isabel Cristina Borges de; Meurer, Rosalva Thereza; Oliveira, André M.

doi:10.1136/jcp.2009.070201

Cited by 109 publications

(70 citation statements)

References 20 publications

(23 reference statements)

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“…Consequently, detection of 12q amplified genes is widely used for distinguishing WDLPS/DDLPS from other tumors. This detection can be done by FISH [15], Q-PCR [42], or aCGH [4] and indirectly by immunohistochemistry [43,44]. MDM2 has early been identified as the main driver gene of the WDLPS/DDLPS amplicon since it is consistently amplified in all cases of WDLPS and DDLPS [10,43,45,46].…”

Section: Discussionmentioning

confidence: 99%

A newly characterized human well-differentiated liposarcoma cell line contains amplifications of the 12q12-21 and 10p11-14 regions

et al. 2012

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While surgery is the usual treatment for localized well-differentiated and dedifferentiated liposarcomas (WDLPS/DDLPS), the therapeutic options for patients with advanced disease are limited. The classical antimitotic treatments are most often inefficient. The establishment of genetically characterized cell lines is therefore crucial for providing in vitro models for novel targeted therapies. We have used spectral karyotyping, fluorescence in situ hybridization with whole chromosome painting and locus-specific probes, and array-comparative genomic hybridization to identify the chromosomal and molecular alterations of a novel cell line established from a recurring sclerosing WDLPS. The karyotype was hypertriploid and showed multiple structural anomalies. All cells retained the presence of a giant marker chromosome that had been previously identified in the primary cell cultures. This giant chromosome contained high-level amplification of chromosomal regions 12q13-21 and lacked the alpha-satellite centromeric sequences associated with WDLPS/DDLPS. The 12q amplicon was large, containing 370 amplified genes. The DNA copy number ranged from 3 to 57. The highest levels of amplification were observed at 12q14.3 for GNS, WIF1, and HMGA2. We analyzed the mRNA expression status by real-time reverse transcription polymerase chain reaction for six genes from this amplicon: MDM2, HMGA2, CDK4, TSPAN31, WIF1, and YEATS4. mRNA overexpression was correlated with genomic amplification. A second amplicon originating from 10p11-14 was also present in the giant marker chromosome. The 10p amplicon contained 62 genes, including oncogenes such as MLLT10, previously described in chimeric fusion with MLL in leukemias, NEBL, and BMI1.

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Section: Discussionmentioning

confidence: 99%

A newly characterized human well-differentiated liposarcoma cell line contains amplifications of the 12q12-21 and 10p11-14 regions

et al. 2012

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“…[8] Fluorescence in situ hybridization (FISH) for MDM2 amplification is a sensitive and specific tool which can be used for distinguishing difficult and doubtful cases of well-differentiated liposarcoma (WDLS). [1,9] In our case, the histopathological and immunohistochemical features were diagnostic and in favor of WDLS.…”

Section: Discussionmentioning

confidence: 78%

“…They are located in the extremities and retroperitoneum in about 70% of cases. [1,2,6,7,9] Paratesticular liposarcomas (PLS), however, are rare malignant tumors which account for approximately 3-7% of all paratesticular sarcomas. [1,3] Most of the available information on these tumors is derived from small series and case reports.…”

Section: Discussionmentioning

confidence: 99%

Liposarcoma scroti: a rare paratesticular tumor

Schoonjans

Servaes

Bronckaers

2016

Acta Chirurgica Belgica

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Paratesticular liposarcoma is a rare condition characterized by a growing, painless inguinal, or scrotal mass. To our knowledge, less than 200 cases worldwide are reported thus far. It is characterized by slow growth and can reach large dimensions at diagnosis. Paratesticular well-differentiated liposarcoma has a prolonged clinical course with recurrences in more than half the cases, sometimes late. Regardless of tumor size, radical orchidectomy with free surgical margins is recommended in order to avoid recurrence. When there is tumor recurrence, reoperation is the treatment of choice because radio- and chemotherapy have yet to be well established in these cases. There is a low risk of metastasis. Overall prognosis is good. Adjuvant therapies, represented by radio- and chemotherapy, have a controversial role in the literature due to the rarity of the disease. In this article, we present review of the literature and case discussion of paratesticular liposarcoma with focus on diagnosis, treatment and follow-up.

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“…Immunostaining with murine double-minute type 2 and cyclin-dependent kinase 4 is useful to exclude liposarcoma. 4 The prognosis is excellent with no reported recurrences after complete resection. Because of the low prevalence of this unusual tumor, it is likely that few cardiologists will encounter this condition.…”

Section: Discussionmentioning

confidence: 98%

A case of a large cardiac lipoma with coronary artery disease

Kanemitsu

Hirano

Shimono

et al. 2014

Asian Cardiovasc Thorac Ann

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Cardiac lipomas are extremely rare benign tumors. They usually remain asymptomatic and are detected incidentally. We report an unusual case of a 60-year-old man who presented with a large epicardial lipoma found unexpectedly during coronary artery examinations. Coronary angiography revealed advanced 3-vessel coronary artery disease. We successfully performed simultaneous curative surgery for the large cardiac lipoma and coronary artery bypass grafting. Histopathology confirmed the diagnosis of lipoma; it weighed 450 g and had a stalk connected to the left atrium.

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Can MDM2 and CDK4 make the diagnosis of well differentiated/dedifferentiated liposarcoma? An immunohistochemical study on 129 soft tissue tumours

Abstract: Detection of MDM2/CDK4 protein overexpression by IHC can be used by pathologists to diagnose WDLPS and DDLPS. Considering a strong and diffuse immunostaining pattern in most of the neoplastic cells achieves the best results in identifying these tumours.

Cited by 109 publications

References 20 publications

A newly characterized human well-differentiated liposarcoma cell line contains amplifications of the 12q12-21 and 10p11-14 regions

A newly characterized human well-differentiated liposarcoma cell line contains amplifications of the 12q12-21 and 10p11-14 regions

Liposarcoma scroti: a rare paratesticular tumor

A case of a large cardiac lipoma with coronary artery disease

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