Can liming reduce cadmium (Cd) accumulation in rice (Oryza sativa) in slightly acidic soils? A contradictory dynamic equilibrium between Cd uptake capacity of roots and Cd immobilisation in soils

Yang, Yongjie; Chen, Jiangmin; Huang, Qi-na; Tang, Shaoqing; Wang, Jianlong; Hu, Peisong; Shao, Gaoneng

doi:10.1016/j.chemosphere.2017.11.061

Cited by 98 publications

(19 citation statements)

References 57 publications

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“…For example, the restoration of Cd-contaminated soil is exceedingly costly and can lead to secondary soil pollution (Zaborowska et al, 2012; Ren et al, 2018). Different cultivation measures are used to address this issue, based on the specific soil types or rice varieties, but their effect is substantially affected by environmental conditions (Li et al, 2015; Yang et al, 2017). Screening for low-Cd-accumulating cultivars requires multi-spot verification over many years, and many screened varieties still have high accumulation of Cd in heavy Cd-contaminated fields (Sun et al, 2016; Gong et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Mutation at Different Sites of Metal Transporter Gene OsNramp5 Affects Cd Accumulation and Related Agronomic Traits in Rice (Oryza sativa L.)

Wang

Fu³

et al. 2019

Front. Plant Sci.

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OsNramp5 is a key gene involved in the control of the uptake of Cd, Mn, and other metal ions by rice root cells. The functional deficiency of this gene can significantly reduce the accumulation of Cd in rice grains, but the effects of its mutation on agronomic traits such as yield and quality have not been investigated comprehensively yet. In the present study, three Huanghuazhan-based OsNramp5 mutants [LCH1 (Low Cadmium Huanghuazhan 1), LCH2 (Low Cadmium Huanghuazhan 2), and LCH3 (Low Cadmium Huanghuazhan 3)] were obtained using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) technology. The mutation-type analysis showed that LCH1, LCH2, and LCH3 encoded defective OsNramp5 protein sequences containing only 76aa, 176aa, and 266aa, respectively. The determination of metal content and the statistics of related agronomic traits revealed that the functionally deficient OsNramp5 not only significantly reduced the accumulation of Cd in the grains of the mutants but also affected rice yield and quality. However, with the decrease of OsNramp5 mutation degree, its effects on chlorenchyma Mn accumulation, yield, and quality were also diminished. Additionally, we also found that the increase in the concentration of Mn in the soil restored the phenotype of the declined yield and quality due to the functional deficiency of OsNramp5. Our findings provide novel insights into and new materials for breeding rice varieties with low Cd accumulation and excellent agronomic traits under severe Cd pollution environment.

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Section: Introductionmentioning

confidence: 99%

Mutation at Different Sites of Metal Transporter Gene OsNramp5 Affects Cd Accumulation and Related Agronomic Traits in Rice (Oryza sativa L.)

Wang

Fu³

et al. 2019

Front. Plant Sci.

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“…Of specific relevance is the decision of the EU regulatory authorities to reduce from 2019 the permissible levels of dietary Cd 2+ (Commission Regulation (EU) no 488/2014). In an attempt to reduce the levels of Cd 2+ in crops, including cacao (the source of chocolate), a variety of research studies are underway to develop agronomic 3 , 4 or genetic 5 solutions to this problem.…”

Section: Introductionmentioning

confidence: 99%

Evolution, and functional analysis of Natural Resistance-Associated Macrophage Proteins (NRAMPs) from Theobroma cacao and their role in cadmium accumulation

Ullah

Wang

Eide

et al. 2018

Sci Rep

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The presence of the toxic metal cadmium (Cd2+) in certain foodstuffs is recognised as a global problem, and there is increasing legislative pressure to reduce the content of Cd in food. The present study was conducted on cacao (Theobroma cacao), the source of chocolate, and one of the crops known to accumulate Cd in certain conditions. There are a range of possible genetic and agronomic methods being tested as a route to such reduction. As part of a gene-based approach, we focused on the Natural Resistance-Associated Macrophage Proteins (NRAMPS), a family of proton/metal transporter proteins that are evolutionarily conserved across all species from bacteria to humans. The plant NRAMP gene family are of particular importance as they are responsible for uptake of the nutritionally vital divalent cations Fe2+, Mn2+, Zn2+, as well as Cd2+. We identified the five NRAMP genes in cacao, sequenced these genes and studied their expression in various organs. We then confirmed the expression patterns in response to variation in nutrient cation availability and addition of Cd2+. Functional analysis by expression in yeast provided evidence that NRAMP5 encoded a protein capable of Cd2+ transport, and suggested this gene as a target for genetic selection/modification.

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“…The expression of OsNRAMP5 also increased by 52.7%-202% in under Mn0.5Cd20, Mn0.5Cd30, Mn1Cd10, Mn1Cd20, Mn1Cd30, Mn1.5Cd10, Mn1.5Cd20, and Mn1.5Cd30 treatments (Fig 6B). It was confirmed that the deficiency of Fe 2+ and M 2+ induced the up-regulation of OsNRAMP5 [53]. However, [43] reported that OsNRAMP5 was not up-regulated under Mn deficiency.…”

Section: Plos Onementioning

confidence: 76%

Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment

Hussain

Ying

et al. 2020

PLoS ONE

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Iron (Fe) and manganese (Mn) are nutritional components of rice, plays an important role in its physiological processes and can minimize absorption of cadmium (Cd) in rice. Fe, Mn, and Cd transporters such as CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, OsHMA3, and OsNAAT1 regulate uptake of Cd in rice. However, the effect of exogenous application of Fe, and Mn on the accumulation of Cd and relative expression (RE) of these transporters in rice has not been investigated. Therefore, a hydroponic culture experiment was conducted to investigate the impact of Fe and Mn on Cd uptake and RE of these transporters in rice. The results showed that the Fe and Mn application significantly decreased Cd in the roots and shoots of rice. Whereas, Cd concentration in the rice significantly increased with increasing Cd concentration in the solution. The addition of manganese in the culture medium can reduce the cadmium content of rice roots by 11.9–82.3% and shoots by 11.6–85.0%, while the addition of iron in the culture medium can reduce the cadmium content of rice roots and shoots by 26–65% and 9–683% respectively. Meanwhile, application of sufficient doses of Fe and Cd in solution culture increased RE of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 in roots, whereas expression level of OsHMA3 was decreased. Similarly, expression level of CAL1, OsNRAMP5, and OsNRAMP1 significantly increased in roots in high Cd and Mn deficient treatments. This may be concluded that the Cd increases expression of CAL1, OsNRAMP5, OsNRAMP1, OsIRT1, and OsNAAT1 but decreases OsHMA3 expression in rice roots, which resulted in increased Cd uptake in hydroponically grown rice.

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Can liming reduce cadmium (Cd) accumulation in rice (Oryza sativa) in slightly acidic soils? A contradictory dynamic equilibrium between Cd uptake capacity of roots and Cd immobilisation in soils

Cited by 98 publications

References 57 publications

Mutation at Different Sites of Metal Transporter Gene OsNramp5 Affects Cd Accumulation and Related Agronomic Traits in Rice (Oryza sativa L.)

Mutation at Different Sites of Metal Transporter Gene OsNramp5 Affects Cd Accumulation and Related Agronomic Traits in Rice (Oryza sativa L.)

Evolution, and functional analysis of Natural Resistance-Associated Macrophage Proteins (NRAMPs) from Theobroma cacao and their role in cadmium accumulation

Effects of Fe and Mn cations on Cd uptake by rice plant in hydroponic culture experiment

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