Can kinematics, file diameter, and PUI influence the intracanal decontamination and apical bacterial extrusion?

Cuéllar, M. R. C.; Velásquez-Espedilla, Evelyn Giuliana; Pedrinha, Victor Feliz; Vivan, Rodrigo Ricci; Duarte, Marco Antônio Húngaro; Andrade, Flaviana Bombarda de

doi:10.1590/1807-3107bor-2021.vol35.0003

Cited by 7 publications

(14 citation statements)

References 35 publications

(62 reference statements)

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“…Absorbent paper point #20 (Dentsply Maillefer, Ballaigues, Switzerland) was inserted into the canals for 1 min each and then transferred to microtubes containing 1 mL of BHI broth according to previous studies. 10,26 The microtubes were vortexed for 10 s, and 100 μL of the content was transferred by dilution to other microtubes with 900 µL of BHI until a concentration of 10 -3 was reached. Next, 100-μL aliquots of the dilutions were plated on Petri dishes with BHI agar, which were stored in a bacteriological incubator at 37°C for 48 h for subsequent CFU/ mL counts.…”

Section: Microbiological Samplesmentioning

confidence: 99%

“…The apical third of the tooth was not used due to the reduced number of dentinal tubules and variable anatomy, as previously observed. 21,24,25,26 In total, 80 images were obtained for each group, as well as 48 images for the positive controls (C + ) and 16 for the negative controls (C -). The images were obtained at a 1-μm step size deep and a resolution of 1024x1024 pixels.…”

Section: Preparation For Confocal Laser Scanning Microscopy (Clsm) Analysismentioning

confidence: 99%

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Impact of irrigation protocols with some chelators and mechanical agitation on intratubular decontamination

et al. 2021

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This study evaluated main canal and intratubular decontamination using different irrigation solutions followed by adjunctive agitation steps for infected root canals. Sixty-eight lower incisors were contaminated with Enterococcus faecalis and allocated to groups according to canal treatment (n = 10): G1, NaOCl followed by ethylenediaminetetraacetic acid (EDTA); G2, a mixture of NaOCl with hydroxyethylidene bisphosphonate (HEBP); and G3, NaOCl followed by EDTA-T (EDTA with sodium lauryl ether sulfate). All three groups of teeth were agitated with passive ultrasonic irrigation (PUI) using saline solution, whereby G4, G5, and G6 were prepared as above, and agitation was performed using an XP-Endo Finisher instrument. Microbiological samples were collected from the root canals with paper points at three times: before and after chemomechanical preparation and after agitation. The colony-forming units (CFU)/mL count was determined, and bacterial intratubular viability was analyzed via confocal laser scanning microscopy using Live/Dead staining. Statistical analysis was performed using a Kruskal-Wallis test followed by Dunn tests. A Friedman test was applied for colony-counting data (α = 0.05). CFU/mL counting indicated equally effective decontamination in the experimental groups (p > 0.05). According to microscopy images, the use of irrigation solutions followed by agitation with the XP-Endo Finisher yielded better results. Moreover, NaOCl+EDTA-T followed by XP-Endo Finisher resulted in significantly lower viability than in the PUI-activated groups (p < 0.05). The cervical and medium thirds of the specimens presented similar results. Overall, NaOCl+EDTA-T exhibited the best intratubular antibacterial activity, mainly for canals that were subsequently agitated using XP-Endo Finisher.

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Section: Microbiological Samplesmentioning

confidence: 99%

Section: Preparation For Confocal Laser Scanning Microscopy (Clsm) Analysismentioning

confidence: 99%

Impact of irrigation protocols with some chelators and mechanical agitation on intratubular decontamination

et al. 2021

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“…The tip diameter of PTU F5 is 0.50 mm, and its taper in the first three millimeters is 0.05 mm. According to a previous study, 20 an increase in file diameter did not reveal a significant difference in AED; however, pressure and friction provided by the file could also affect the amount of AED.…”

Section: Discussionmentioning

confidence: 68%

“…During extrusion experiments, primarily single-rooted premolar teeth were used. 8 , 9 , 11 , 13 , 19 , 20 The mean minimum diameter of the apical foramina of maxillary palatal roots was greater than of mandibular premolar roots, 30 and an increase in foramen diameter increases the risk of extrusion. 31 In addition, maxillary molars have a complex root canal anatomy 32 that reduces the success rate of initial treatment, and their roots may be located in the maxillary sinus, which increases the extrusion potential of filling materials, irrigants, and tissue debris.…”

Section: Discussionmentioning

confidence: 99%

“… 3 , 4 , 18 , 19 Previous research found no significant difference between the kinematic design of the files or the tip diameter and the amount of microorganisms extruded from the apical foramen. 20 Furthermore, the effect of obturation materials and techniques on the quantity of AED has been studied several times. 7 , 10 , 21 , 22 However, data regarding the effect of initial enlargement on AED during the removal of preceding filling materials are limited.…”

Section: Introductionmentioning

confidence: 99%

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In vitro assessment of the roles of initial preparation size and solvent application on apically extruded debris in endodontically treated teeth

Yavaş,

Yekeler,

İlgen

et al. 2023

J Dent Res Dent Clin Dent Prospects

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Background. This study investigated the effects of solvent use and initial canal enlargement size on apically extruded debris (AED). Methods. The palatal roots of 60 upper molars were assigned to two groups based on the initial preparation size: F3 or F4 ProTaper Universal files. The roots were obturated using the single-cone technique. Each group was subdivided based on the retreatment procedures: with or without solvent. After evaporation, the Eppendorf tubes were weighed using an electronic balance. The data were statistically analyzed (P=0.05). Results. Using a solvent enhanced the amount of AED in samples initially prepared up to F3 or F4 files (P<0.05). When the solvent was used, the AED was greater in samples initially prepared up to the F4 file (P<0.05); however, when the solvent was omitted, the results were reversed (P<0.05). Conclusion. Both parameters had a role in AED. Solvents should be used cautiously during root canal retreatment because of the potential for apical extrusion of filling materials. Using files with smaller tapers during initial preparation might be beneficial when considering the amount of AED compared to those with larger tapers.

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Influence of pecking frequency at working length on the volume of apically extruded debris: A micro-computed tomography analysis

Wang

Chen

Lin

et al. 2022

Journal of Dental Sciences

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Can kinematics, file diameter, and PUI influence the intracanal decontamination and apical bacterial extrusion?

Cited by 7 publications

References 35 publications

Impact of irrigation protocols with some chelators and mechanical agitation on intratubular decontamination

Impact of irrigation protocols with some chelators and mechanical agitation on intratubular decontamination

In vitro assessment of the roles of initial preparation size and solvent application on apically extruded debris in endodontically treated teeth

Influence of pecking frequency at working length on the volume of apically extruded debris: A micro-computed tomography analysis

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