Can just one-second measurement of chlorophyll a fluorescence be used to predict sulphur deficiency in radish (Raphanus sativus L. sativus) plants?

Samborska, Izabela A.; Kalaji, Hazem M.; Sieczko, Leszek; Borucki, Wojciech; Mazur, Radosław; Kouzmanova, Margarita; Goltsev, Vasilij

doi:10.1016/j.cpb.2018.12.002

Cited by 29 publications

(28 citation statements)

References 27 publications

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“…4b) due to higher energy dissipation from the active RCs under As toxicity 14 . Furthermore, increased F o /F v refers to damaging effects on OEC, which could be due to the decline in uptake of mineral nutrients like Mn, an important component of OEC 6 , as it suggested by Samborska et al 24 that mineral nutrient deficiency tends to affect the fluorescence parameters variably.…”

Section: Resultsmentioning

confidence: 95%

“…4a). The decline in O-J-I-P transient curve could be attributed to damage at the donor side of PS II restricting the flow of electron between OEC and PS II [20][21][22][23][24] . Moreover, the drop in OJIP transient curves was intense upon c-PTIO + l-NAME supplementation to As + Ca treated seedlings indicating that in absence of NO, the effect of As became more severe (even in presence of Ca 2+ ), which could be due to: (i) inhibition in electron transport rate on the donor side of PS II as reflected by decreased values for area over the fluorescence curve (Area), which consequently decreased the maximum quantum yield for primary photochemistry www.nature.com/scientificreports www.nature.com/scientificreports/ (that designates trapped exciton moves an electron into the ETC beyond Q A − ) and the quantum yield of electron transport (ФE o ) was detected ( Fig.…”

Section: Resultsmentioning

confidence: 99%

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Interplay of Calcium and Nitric Oxide in improvement of Growth and Arsenic-induced Toxicity in Mustard Seedlings

Singh

Parihar

Prasad

2020

Sci Rep

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In this study, Ca 2+ mediated no signalling was studied in response to metalloid (As) stress in Brassica seedlings. Arsenic toxicity strongly suppressed the growth (fresh weight, root and shoot length), photosynthetic pigments, Chl a fluorescence indices (Kinetic traits: F v , F m , F v /F o , F m /F o , ФP o or F v /F m , Ψ o , ФE o , PI ABS , Area and N and redox status (AsA/DHA and GSH/GSSG ratios) of the cell; whereas energy flux traits: ABS/RC, TR o /RC, ET o /RC and DI o /RC along with F o , F o /F v , F o /F m , ФD o and S m ) were enhanced. Further, addition of EGTA (Ca 2+ scavenger) and Lacl 3 (plasma membrane ca 2+ channel blocker) to As + ca; while c-ptio (no scavenger) and l-nAMe (no synthase inhibitor) to As + Snp treated seedlings, siezed recovery on above parameters caused due to Ca 2+ and NO supplementation, respectively to As stressed seedlings thereby indicating their signalling behaviour. Further, to investigate the link between ca 2+ and NO, when c-ptio and l-nAMe individually as well as in combination were supplemented to As + ca treated seedlings; a sharp inhibition in above mentioned traits was observed even in presence of ca 2+ , thereby signifying that NO plays crucial role in Ca 2+ mediated signalling. In addition, As accumulation, ROS and their indices, antioxidant system, NO accumulation and thiol compounds were also studied that showed varied results.Arsenic (As) exposure has become a major threat for world agriculture that causes adverse effect on crop productivity by inhibiting cell functioning. The metalloid (As) mainly arises from geothermal weathering of rocks and human activities 1 . The toxicity of As depends on As species. Among the two inorganic species, arsenite (As III ), which prevails in anaerobic environment, enters in plant system through aquaporin channels with greater affinity for thiol groups; while arsenate (As V ) prevalent in aerobic condition/ soil, being analogue of inorganic phosphate (iP), enters via inorganic phosphate transporters, competes with phosphate and replaces phosphate from ATP thereby affecting the energy metabolism of cell 2,3 . Arsenic accumulation causes growth suppression which involves many biochemical and physiological changes in plants including oxidative stress, injuries to membrane and thereby affecting redox state of the cell 4-6 , which is detrimental for plant survival under As toxicity. Additionally, As toxicity negatively regulates chlorophyll (Chl) biosynthesis, PS II photochemistry and ribulose 1,5-bisphosphate carboxylase/ oxygenase (RuBisCO) activity, thereby inhibiting photosynthetic efficiency 6-9 . Previous studies have explicated the prominence of upholding a favourable antioxidants level and redox status of cell to encounter the damage caused due to metalloid exposure 4-6 .Calcium (Ca 2+ ) is a pervasive and pivotal secondary messenger in signal transduction network 10 under both stressed and non-stressed situations. In several studies, it has been shown that Ca 2+ is involved in regulation of plant responses such as photosyn...

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Interplay of Calcium and Nitric Oxide in improvement of Growth and Arsenic-induced Toxicity in Mustard Seedlings

Singh

Parihar

Prasad

2020

Sci Rep

View full text Add to dashboard Cite

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“…In previous review on the JIP-test applicability, Strasser et al (2004) suggested the calculation of H and G bands, which have not been well studied so far. We used ΔWJI and ΔWIP normalization (used by Samborska et al 2019) to study these phases in detail, and we labelled them H and G band, respectively. H band gives information about the electron flow to the end acceptors in PSII, strongly correlated with the PQ pool size and the degree of its reduction.…”

Section: Resultsmentioning

confidence: 99%

Special issue in honour of Prof. Reto J. Strasser - Photosynthetic efficiency of two Platanus orientalis L. ecotypes exposed to moderately high temperature - JIP-test analysis

Dimitrova¹,

Paunov²,

Pavlova³

et al. 2020

Photosynt.

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Plane (Platanus orientalis) is a popular park tree in Europe but is almost extinct in natural ecosystems, because of climate changes. In our study, two ecotypes of plane tree (Bulgarian and Italian) were submitted to moderately elevated temperature that occurs in summer. Our aim was to compare stress reactions, tolerance, and adaptability of these plants. Leaf age had a significant impact on the stress effects. For correct interpretation of the results, we investigated leaves at different positions from the apical bud, i.e., leaves of different age. We assessed their photosynthetic efficiency at room temperature and after treatment at moderately high temperature by simultaneous measurement of prompt and delayed chlorophyll fluorescence, as well as light reflection at 820 nm. For more precise interpretation of the obtained results we did principal component analysis. The two studied plane ecotypes showed different tolerance to the elevated temperature. Plants of Italian ecotype showed better adaptivity and developed advantageous photosynthetic characteristics, while Bulgarian ecotype was more affected. Plants of both ecotypes recovered from the heat stress.

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“…The JIP-test allows one to evaluate the efficiency of electron transfer stages using the parameters of the induction curve. This method has been successfully applied for the analysis of effects of macroand micronutrient deficiency on the functioning of the photosynthetic machinery in tomato and maize plants (Kalaji et al 2014), sulfur deficiency effects on Raphanus sativus (Samborska et al 2019), and nitrogen deficiency in Actinidia arguta (Swoczyna et al 2019). Solovchenko et al (2013) studied the applicability of OJIP parameters for online monitoring of physiological state and accumulation of lipids in microalgal cultures grown in photobioreactors.…”

Section: Introductionmentioning

confidence: 99%

Special issue in honour of Prof. Reto J. Strasser - Gradual changes in the photosynthetic apparatus triggered by nitrogen depletion during microalgae cultivation in photobioreactor

Plyusnina¹,

Khruschev²,

Degtereva³

et al. 2020

Photosynt.

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Changes of photosynthetic activity during natural depletion of nitrogen in a batch culture of Chlorella vulgaris were studied using chlorophyll a fluorescence-based methods. Complex analysis including JIP-test, multiexponential approximation, and the analysis of differential curves was carried out on the recorded fluorescence transients. Three pivot points were detected in dynamics of the JIP-test parameters during culture growing. We associated these time points with different stages of mineral stress progress. Immediately after nitrogen exhaustion in the cultivation medium, a transient increase in the efficiency of PSII was detected. During this short period, the photosynthetic apparatus acclimated to the stress by reducing the number of PSII reaction centers, simultaneously increasing the effective cross section of the light-harvesting antenna per reaction center. However, prolonged nutrient starvation impaired the structure of the photosynthetic apparatus, deactivating the oxygen-evolving complex and impairing the overall electron transport.

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Can just one-second measurement of chlorophyll a fluorescence be used to predict sulphur deficiency in radish (Raphanus sativus L. sativus) plants?

Cited by 29 publications

References 27 publications

Interplay of Calcium and Nitric Oxide in improvement of Growth and Arsenic-induced Toxicity in Mustard Seedlings

Interplay of Calcium and Nitric Oxide in improvement of Growth and Arsenic-induced Toxicity in Mustard Seedlings

Special issue in honour of Prof. Reto J. Strasser - Photosynthetic efficiency of two Platanus orientalis L. ecotypes exposed to moderately high temperature - JIP-test analysis

Special issue in honour of Prof. Reto J. Strasser - Gradual changes in the photosynthetic apparatus triggered by nitrogen depletion during microalgae cultivation in photobioreactor

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