Can introgression in M-lineage honey bees be detected by abdominal colour patterns?

Henriques, Dora; Lopes, Ana Rita; Ferrari, Roberto; Neves, Cátia J.; Quaresma, Andreia; Browne, Keith A.; McCormack, Grace P.; Pinto, M. Alice

doi:10.1007/s13592-020-00744-7

Cited by 2 publications

(3 citation statements)

References 44 publications

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“…Our results on these simulated hybrids provided insights on the applicability of the 96 AIM panel and we found that it was effective to detect recent cases of admixture as long as (1) admixed samples identified as such are present in the training set, and (2) admixture is relatively recent. In our tests, the classification was perfect up to the 2nd generation when crossing with the M lineage and up to the 4th generation with the C lineage, a type of admixture that is more likely to occur [ 34 ]. Also of interest, the comparison of the selected GI1 list with all the variants (see Additional file 1 : Fig.…”

Section: Discussionmentioning

confidence: 99%

Natural clines and human management impact the genetic structure of Algerian honey bee populations

Salvatore,

Chibani Bahi Amar,

Canale-Tabet

et al. 2023

Genet Sel Evol

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Background The Algerian honey bee population is composed of two described subspecies A. m. intermissa and A. m. sahariensis, of which little is known regarding population genomics, both in terms of genetic differentiation and of possible contamination by exogenous stock. Moreover, the phenotypic differences between the two subspecies are expected to translate into genetic differences and possible adaptation to heat and drought in A. m. sahariensis. To shed light on the structure of this population and to integrate these two subspecies in the growing dataset of available haploid drone sequences, we performed whole-genome sequencing of 151 haploid drones. Results Integrated analysis of our drone sequences with a similar dataset of European reference populations did not detect any significant admixture in the Algerian honey bees. Interestingly, most of the genetic variation was not found between the A. m. intermissa and A. m. sahariensis subspecies; instead, two main genetic clusters were found along an East–West axis. We found that the correlation between genetic and geographic distances was higher in the Western cluster and that close-family relationships were mostly detected in the Eastern cluster, sometimes at long distances. In addition, we selected a panel of 96 ancestry-informative markers to decide whether a sampled bee is Algerian or not, and tested this panel in simulated cases of admixture. Conclusions The differences between the two main genetic clusters suggest differential breeding management between eastern and western Algeria, with greater exchange of genetic material over long distances in the east. The lack of detected admixture events suggests that, unlike what is seen in many places worldwide, imports of queens from foreign countries do not seem to have occurred on a large scale in Algeria, a finding that is relevant for conservation purposes. In addition, the proposed panel of 96 markers was found effective to distinguish Algerian from European honey bees. Therefore, we conclude that applying this approach to other taxa is promising, in particular when genetic differentiation is difficult to capture.

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Section: Discussionmentioning

confidence: 99%

Natural clines and human management impact the genetic structure of Algerian honey bee populations

Salvatore,

Chibani Bahi Amar,

Canale-Tabet

et al. 2023

Genet Sel Evol

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“…Coloration is assessed at the first, second, and third abdominal tergites. The degree of coloration is assessed using different scales [7,8,14,15] as described by Ruttner [2]. Coloration patterns are classified into groups based on the extent to which changes are observed.…”

Section: Introductionmentioning

confidence: 99%

“…For scientific purposes, ten-grade scales are used [4,5], which enables us to capture more natural variability. For use in breeding programs, a four-grade scale is commonly used [14,15]. The highest grade means no yellow marks in abdominal tergites, and the lowest grade marks yellow bands on the first, second, third, and sometimes even on the fourth abdominal tergite.…”

Section: Introductionmentioning

confidence: 99%

Quantifying Abdominal Coloration of Worker Honey Bees

Bubnič,

Prešern

2024

Insects

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The main drawback in using coloration to identify honey bee subspecies is the lack of knowledge regarding genetic background, subjectivity of coloration grading, and the effect of the environment. The aim of our study was to evaluate the effect of environmental temperature on the abdominal coloration of honey bee workers and to develop a tool for quantifying abdominal coloration. We obtained four frames of honey bee brood from two colonies and incubated them at two different temperatures (30 and 34 °C). One colony had workers exhibiting yellow marks on the abdomen, while the other did not. We collected hatched workers and photographed abdomens. Images were analyzed using custom-written R script to obtain vectors that summarize the coloration over the abdomen length in a single value—coloration index. We used UMAP to reduce the dimensions of the vectors and to develop a classification procedure with the support vector machine method. We tested the effect of brood origin and temperature on coloration index with ANOVA. UMAP did not distinguish individual abdomens according to experimental group. The trained classifier sufficiently separated abdomens incubated at different temperatures. We improved the performance by preprocessing data with UMAP. The differences among the mean coloration index values were not significant between the gray groups incubated at different temperatures nor between the yellow groups. However, the differences between the gray and yellow groups were significant, permitting options for application of our tool and the newly developed coloration index. Our results indicate that the environmental temperature in the selected range during development does not seem to impact honey bee coloration significantly. The developed color-recording protocol and statistical analysis provide useful tools for quantifying abdominal coloration in honey bees.

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Can introgression in M-lineage honey bees be detected by abdominal colour patterns?

Cited by 2 publications

References 44 publications

Natural clines and human management impact the genetic structure of Algerian honey bee populations

Natural clines and human management impact the genetic structure of Algerian honey bee populations

Quantifying Abdominal Coloration of Worker Honey Bees

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