Can Hot-Water Emasculation Be Applied to Artificial Hybridization of Indica—Type Cambodian Rice?

Tong, Ly; Yoshida, Tomohiko

doi:10.1626/pps.11.132

Cited by 10 publications

(8 citation statements)

References 2 publications

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“…Emasculation with water at 45°C achieved a lower selfpollination percentage than at lower temperatures (Table 2). Tong and Yoshida (2008) similarly reported effective rice emasculation at 45°C and 46°C. Therefore, if the breeder does not have time to test the best water temperature for emasculating a variety, it would be safer to use it.…”

Section: Discussionmentioning

confidence: 82%

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Efficiency of different hybridization methods in single crosses or rice for pure line breeding

García-Yzaguirre¹,

Carreres

2008

Span. j. agric. res.

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Single crosses are usually made for combining different characters from two varieties or species, at the F1 generation in a crop breeding program. Afterwards, F1 plants have to be checked out for hybridity.In rice (Oryza sativa L.), Baldi (1967) found that hand emasculation followed by pollination with anthers had a lower seed set than emasculation with water at 41-43.5°C for 5 or 8 min (which opens mature florets), followed by bagging two panicles together for pollination. This author reported varietal differences in seed set but he did not determine self-pollination rates. Water temperature should be high enough to kill pollen, but not so high as to kill ovaries. In contrast, Jennings et al. (1979), at IRRI (International Rice Research Institute, Philippines), claimed that emasculation at hot temperatures was slower and had lower seed set than hand emasculation or anther aspiration, apparently because culms were bent to introduce panicles Efficiency of different hybridization methods in single crosses of rice for pure line breedingA. García-Yzaguirre* and R. CarreresDepartamento del Arroz (IVIA). Apdo. 37. 46410 Sueca (Valencia). Spain AbstractHybridization efficiency of two crossing methods (involving emasculation by hot water or by anther aspiration), was determined in rice. Quantitative comparisons between crossing methods for pure line breeding are scarce and the objectives of this work were to evaluate the advantages and disadvantages of emasculation methods, and to determine an adequate temperature for hot water emasculation. Hot water emasculation at a temperature suitable to each variety (followed by panicle pollination) achieved a higher percentage of hybrid F1 plants (54.6%) than anther aspiration (8.4%), but significantly more seed set was obtained after anther aspiration (31.5%) than after hot water immersion (16.5%). The easiest and quickest emasculation method was the immersion in hot water of panicles from detached rooted tillers.Additional key words: crop improvement, crossing, emasculation techniques, Oryza sativa L. Resumen Eficiencia de distintos métodos de hibridación en cruzamientos de arroz para obtención de líneas purasSe determinó la eficiencia de dos métodos de cruzamiento en arroz, implicando emasculación (castración) por agua caliente o por aspiración de anteras. Las comparaciones cuantitativas entre métodos de cruzamiento para obtención de líneas puras son escasas y por ello los principales objetivos de este trabajo fueron evaluar las ventajas y desventajas de ambos métodos de emasculación, así como determinar una temperatura adecuada de castración con agua caliente. La emasculación por agua caliente a una temperatura adecuada para cada variedad (seguida de polinización con panícula) obtuvo un mayor porcentaje de plantas F1 híbridas (54,6%) que la aspiración de anteras (8,4%), pero se obtuvieron significativamente más semillas tras aspiración de anteras (31,5%) que tras inmersión en agua caliente (16,5%). El método más fácil y rápido de emasculación fue la inmersión en agua caliente...

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Section: Discussionmentioning

confidence: 82%

“…Panicle emasculation with hot water (thermal emasculation): Matsubayashi et al, 1965, cited by Tong andYoshida, 2008;Baldi, 1967. Preliminary tests of hot water emasculation in our department showed that emasculation at 41°C produced about 70% selfpollinated seed and that emasculation at 47°C was lethal for the ovary in most cases.…”

Section: Methodsmentioning

confidence: 99%

Efficiency of different hybridization methods in single crosses or rice for pure line breeding

García-Yzaguirre¹,

Carreres

2008

Span. j. agric. res.

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“…In Cambodia rice cultivars, thermal emasculation can be applied for artificial hybridization between the Indica and Japonica genotype groups under the conditions of 43°C for 7 minutes [4]. Even though the present work has similar temperatures and emasculation time to the experiments of these aforementioned authors, the results are different because a higher percentage of grains was obtained.…”

Section: Formosa Cultivars Setting the Sculation By Hyperthermotherapymentioning

confidence: 73%

“…The male gametophyte is particularly sensitive to high temperatures at all stages of development, whereas the pistil and the female gametophyte are considered more tolerant [3]. Hyperthermotherapy is easy to perform, less labor intensive, and does not necessarily require skilled labor [4].…”

Section: Introductionmentioning

confidence: 99%

Hyperthermotherapy in the Rice Emasculation Process

Aguiar

Streck

Facchinello

et al. 2017

JEAI

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Aims: The objective of this work was to evaluate the emasculation by hyperthermotherapy method in irrigated rice genotypes, and to compare it with the emasculation by vacuum suction. Study Design: The experimental design used to compare the two methods was completely randomized, with two treatments (emasculation by hyperthermotherapy and vacuum suction) and six replications. To analyze the emasculation by hyperthermotherapy, this design was also used with three replications.

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“…A total of 12 landraces and 18 rice varieties were screened for PD soil conditions and subjected to three-tier indexation of 3: tolerant; 2: moderately tolerant and 1: sensitive (Aluwihare et al, 2016). Approximately 84 crosses were made between tolerant and moderately tolerant / sensitive rice genotypes using clipping and hot water dipping methods (Tong and Yoshida, 2008). A total of three crosses namely H-4 × Bg 357, Murungakayan × Bg 357 and Marss × Bg 357 were selected for the present study based on the degree of tolerance and importance to breeding.…”

Section: Plant Materialsmentioning

confidence: 99%

Screening of segregating F2 progenies and validation of DNA markers through bulk segregant analysis for phosphorous deficiency tolerance in rice

Aluwihare¹,

Chamikara²,

Karannagoda³

et al. 2016

Ceylon J. Sci.

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Phosphorous deficiency (PD) tolerance is a polygenic trait. The underlying genetics of PD tolerance trait is important to provide the basis for detecting Quantitative Trait Loci (QTLs) and validating markers that could be used in Marker Assisted Breeding (MAB) in rice. The PD tolerance of Sri Lankan rice germplasm has been characterized. However, no attempts were taken to develop and validate the DNA markers for the breeding purposes and to understand the genetic basis of the traits. The present research project was conducted to assess the PD related traits and to validate internationally published DNA markers that are linked to PD tolerance using Sri Lankan rice cultivars. A total of 84 crosses were made and advanced to F 2 and higher generations. Out of these crosses, an important subset of three crosses was selected based on the overall PD tolerance and sensitivity, importance as mega production varieties and pedigree connections between the cultivars. The plant height, number of tillers, shoot dry weight, leaf width, flag leaf width and the color metrics L*, a*, b*, hue (h*) and chroma (C*) were measured from 200 individuals each from the three populations grown under P deficient (P o) soil conditions. Except color traits, other traits were normally distributed and exhibited higher broad sensitivity. The color metrics indicate the presence of possible epistatic interactions between the major underlying loci. From each population, two extreme bulks were selected from the highest and lowest ends of shoot dry weight (SDW) for bulk segregant analyses (BSA) to validate the DNA markers for PD tolerance. It was observed that, DNA marker K46-K1 can be used for MAB of rice for PD tolerance. The genetic information generated in the present study can also be used for larger scale genomic studies such as SNPs, GBS and GWAS mapping.

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Can Hot-Water Emasculation Be Applied to Artificial Hybridization of Indica—Type Cambodian Rice?

Cited by 10 publications

References 2 publications

Efficiency of different hybridization methods in single crosses or rice for pure line breeding

Efficiency of different hybridization methods in single crosses or rice for pure line breeding

Hyperthermotherapy in the Rice Emasculation Process

Screening of segregating F2 progenies and validation of DNA markers through bulk segregant analysis for phosphorous deficiency tolerance in rice

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