Can half-marathon affect overall health? The yin-yang of sport

Carbonare, Luca Dalle; Manfredi, Marcello; Caviglia, Giuseppe; Conte, Eleonora; Robotti, Elisa; Marengo, Emílio; Cheri, Samuele; Zamboni, Francesco; Gabbiani, Daniele; Deiana, Michela; Cecconi, Daniela; Schena, Federico; Mottes, Monica; Valenti, Maria Teresa

doi:10.1016/j.jprot.2017.09.004

Cited by 24 publications

(23 citation statements)

References 56 publications

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“…Recently, we identified several proteins differentially expressed in runners' sera after the half marathon. Despite several negative cellular effects, such as oxidative stress, the resulting proteome modulation seems to be beneficial for the overall health [5]. We also demonstrated that running the half marathon increases the expression of the osteogenic transcription factor RUNX2 as well as the calcification process in an in vitro model.…”

Section: Introductionmentioning

confidence: 61%

Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity

Carbonare

Mottes

Cheri

et al. 2019

Oxidative Medicine and Cellular Longevity

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Lack of physical exercise is considered an important risk factor for chronic diseases. On the contrary, physical exercise reduces the morbidity rates of obesity, diabetes, bone disease, and hypertension. In order to gain novel molecular and cellular clues, we analyzed the effects of physical exercise on differentiation of mesenchymal circulating progenitor cells (M-CPCs) obtained from runners. We also investigated autophagy and telomerase-related gene expression to evaluate the involvement of specific cellular functions in the differentiation process. We performed cellular and molecular analyses in M-CPCs, obtained by a depletion method, of 22 subjects before (PRE RUN) and after (POST RUN) a half marathon performance. In order to prove our findings, we performed also in vitro analyses by testing the effects of runners' sera on a human bone marrow-derived mesenchymal stem (hBM-MSC) cell line. PCR array analyses of PRE RUN versus POST RUN M-CPC total RNAs put in evidence several genes which appeared to be modulated by physical activity. Our results showed that physical exercise promotes differentiation. Osteogenesis-related genes as RUNX2, MSX1, and SPP1 appeared to be upregulated after the run; data showed also increased levels of BMP2 and BMP6 expressions. SOX9, COL2A1, and COMP gene enhanced expression suggested the induction of chondrocytic differentiation as well. The expression of telomerase-associated genes and of two autophagy-related genes, ATG3 and ULK1, was also affected and correlated positively with MSC differentiation. These data highlight an attractive cellular scenario, outlining the role of autophagic response to physical exercise and suggesting new insights into the benefits of physical exercise in counteracting chronic degenerative conditions.

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Section: Introductionmentioning

confidence: 61%

Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity

Carbonare

Mottes

Cheri

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Recently, research interest in iron metabolism during and after exercise has grown because physical activity can affect iron and iron-regulatory protein status in many ways, such as by inducing oxidative stress and inflammation [5,6,7,8,9,10]. In the case of intense running efforts, foot strike causes hemolysis as an additional factor that contributes to disordered iron metabolism [11,12].…”

Section: Introductionmentioning

confidence: 99%

Changes in Serum Iron and Leukocyte mRNA Levels of Genes Involved in Iron Metabolism in Amateur Marathon Runners—Effect of the Running Pace

Grzybkowska

Anczykowska

Ratkowski

et al. 2019

Genes

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Iron is essential for physical activity due to its role in energy production pathways and oxygen transportation via hemoglobin and myoglobin. Changes in iron-related biochemical parameters after physical exercise in athletes are of substantial research interest, but molecular mechanisms such as gene expression are still rarely tested in sports. In this paper, we evaluated the mRNA levels of genes related to iron metabolism (PCBP1, PCBP2, FTL, FTH, and TFRC) in leukocytes of 24 amateur runners at four time points: before, immediately after, 3 h after, and 24 h after a marathon. We measured blood morphology as well as serum concentrations of iron, ferritin, and C-reactive protein (CRP). Our results showed significant changes in gene expression (except for TFRC), serum iron, CRP, and morphology after the marathon. However, the alterations in mRNA and protein levels occurred at different time points (immediately and 3 h post-run, respectively). The levels of circulating ferritin remained stable, whereas the number of transcripts in leukocytes differed significantly. We also showed that running pace might influence mRNA expression. Our results indicated that changes in the mRNA of genes involved in iron metabolism occurred independently of serum iron and ferritin concentrations.

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“…In addition, MSCs are also the precursor cells of adipocytes and peroxisome proliferator-activated receptor gamma (PPARγ) is the transcription factor essential for adipogenic differentiation [7]. Importantly, reciprocal control between osteogenic and adipogenic differentiation occurs [8,9], and we can suppose that hormonal vitamin D, via inducing osteogenic maturation, could influence adipogenic differentiation.…”

Section: Introductionmentioning

confidence: 99%

Vitamin D Daily versus Monthly Administration: Bone Turnover and Adipose Tissue Influences

et al. 2018

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Vitamin D is involved in bone metabolism and in many various extra-skeletal diseases such as malabsorption syndromes, cardiovascular and metabolic diseases, cancer, and autoimmune and neurological diseases. However, data on the optimal route of administration are not consistent. The aims of our study were to analyze not only the influence of daily vs. monthly administration of vitamin D on bone metabolism and bone turnover, but also the effects of different routes of administration on fat mass in a cohort of adults with low levels of 25(OH) vitamin D3 at baseline. We analyzed 44 patients with hypovitaminosis at baseline and after six months of two different regimens of administration: seven drops (1750 IU)/day vs. 50,000 IU/month. We found that the two regimens were equivalent; 36 out of 44 patients reached the normal range of vitamin D after six months of treatment. Interestingly, the main determinant of vitamin D at baseline was the waist circumference. In addition, 22 patients treated by monthly regimen were evaluated after 18 months of treatment. At the end of follow-up, patients showed normal levels of vitamin D, with increased calcium levels and decreased bone turnover. Waist circumference also decreased. Our results support the efficacy of vitamin D3 given monthly both for correcting hypovitaminosis and for maintaining vitamin D levels. The relationship between serum 25(OH)vitamin D3 concentration and waist circumference supports vitamin D having a protective role in the current setting, since waist size is directly associated with the risk of cardiovascular and metabolic diseases.

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Can half-marathon affect overall health? The yin-yang of sport

Cited by 24 publications

References 56 publications

Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity

Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity

Changes in Serum Iron and Leukocyte mRNA Levels of Genes Involved in Iron Metabolism in Amateur Marathon Runners—Effect of the Running Pace

Vitamin D Daily versus Monthly Administration: Bone Turnover and Adipose Tissue Influences

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