Can free DNA be detected in sputum of lung cancer patients?

Drift, Miep A. van der; Prinsen, Clemens; Hol, Bernard; Bolijn, Anne S.; Jeunink, Marcel; Dekhuijzen, P.N.R.; Thunnissen, Erik

doi:10.1016/j.lungcan.2008.01.007

Cited by 39 publications

(27 citation statements)

References 25 publications

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“…For the quantification of DNA, we selected a real-time PCR assay designed for the ␤-globin sequence that performed consistently in other experiments [29,33]. Real-time PCR for DNA quantification can be regarded as the standard method [34].…”

Section: Discussionmentioning

confidence: 99%

Circulating DNA is a non-invasive prognostic factor for survival in non-small cell lung cancer

et al. 2010

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Section: Discussionmentioning

confidence: 99%

Circulating DNA is a non-invasive prognostic factor for survival in non-small cell lung cancer

et al. 2010

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“…1 The GoldenGate Assay was used to detect methylation of 1,505 CpG sites in promoter regions of 807 genes. Hybridization of the DNA to a set of allele specific oligonucleotide and locus-specific oligonucleotide was dependent on the methylation status at the CpG site of interest.…”

Section: Methodsmentioning

confidence: 99%

“…In particular, evaluation of fluids approximating the cancer has significant clinical applicability. For example, sputum analysis has been used to detect lung carcinoma (1). In similar fashion, saliva is the proximal fluid for head and neck squamous cell carcinoma (SCC).…”

Section: Introductionmentioning

confidence: 99%

Methylation Array Analysis of Preoperative and Postoperative Saliva DNA in Oral Cancer Patients

Viet

Schmidt

2008

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Purpose: To perform methylation array analysis of 807 cancer-associated genes using tissue and saliva of oral squamous cell carcinoma (OSCC) patients with the objective of identifying highly methylated gene loci that hold diagnostic and predictive value as a biomarker. Experimental Design: We did the methylation array on DNA extracted from preoperative saliva, postoperative saliva, and tissue of 13 patients with OSCC, and saliva of 10 normal subjects. We identified sites that were highly methylated in the tissue and preoperative saliva samples but not methylated in the postoperative saliva samples or in normal subjects.Results: High quality DNA was obtained and the methylation array was successfully run on all samples. We identified significant differences in methylation patterns between the preoperative and postoperative saliva from cancer patients. We established a gene classifier consisting of 41 gene loci from 34 genes that showed methylation in preoperative saliva and tissue but were not methylated in postoperative saliva or normal subjects. Gene panels of 4 to 10 genes were constructed from genes in the classifier. The panels had a sensitivity of 62% to 77% and a specificity of 83% to 100% for OSCC. Conclusions: We report methylation array analysis of 807 cancer-associated genes in the saliva of oral cancer patients before and after oral cancer resection. Our methylation biomarker approach shows the proof of principle that methylation array analysis of saliva can produce a set of cancer-related genes that are specific and can be used as a composite biomarker for the early detection of oral cancer. (Cancer Epidemiol Biomarkers Prev 2008;17(12):3603 -11)

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“…When cfDNA had been quantified it was shown that the amount is increased in a variety of different conditions such as myocardial infarction [14], cardiac arrest [15], exhaustive exercise [16][17][18], in patients with systemic lupus erythematosis [19], in older humans [20], in febrile patients [21], in children on peritoneal dialysis [22], in patients with obstructive sleep apnea [23], patients with chronic kidney disease [24], patients with severe sepsis or septic shock [25], in trauma [26] and burn patients [27] (chapter "CNAPS and General Medicine"). In an attempt to differentiate lung cancer patients from a control population according to their sputum cfDNA it was demonstrated that the amount of cfDNA was related to the severity of the inflammatory processes but not the presence of lung cancer [28]. When a capillary electrophoresis (CE) method and qPCR were used to determine the amount of plasma cfDNA in non-small cell lung cancer (NSCLC) patients and healthy controls, the quantity of cfDNA obtained with CE was almost twice as high as with qPCR and with both methods, almost twice as high in NSCLC patients when compared with a group of healthy subjects.…”

Section: Dna Quantification/dna Integritymentioning

confidence: 99%

Extracellular Nucleic Acids and Cancer

Fleischhacker¹,

Schmidt²

2014

Advances in Predictive, Preventive and Personalised Medicine

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Since the clear proof of the presence of tumor-associated genetic alterations in extracellular nucleic acids almost 20 years ago this field gained has attracted much interest. According to our current knowledge it seems as if all tumor-associated alterations found in tumor cells are also found in the extracellular environment. The isolation of extracellular nucleic acids from tumor patients and its genetic characterization with very sensitive and highly specific methods led to the concept of "liquid biopsy". This means that for follow-up analysis of tumor patients, the physicians no longer depend exclusively on a single examination of tissue biopsies (usually at the time of diagnosis) but are able by longitudinally analyzing the extracellular nucleic acids to follow the reaction of a tumor to e.g. a given therapy, the development of resistance mechanisms. In this chapter we will discuss the detection and characterization of different genetic (e.g. mutation analysis and structural variations as seen in microsatellites), epigenetic (e.g. hypermethylation of selected sequences) and regulatory alterations (as in different miRNA expression patterns found in tumor patients). We will also touch on some confounding factors that have to be taken into consideration as well as the functional and biological aspects of extracellular nucleic acids.

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Can free DNA be detected in sputum of lung cancer patients?

Cited by 39 publications

References 25 publications

Circulating DNA is a non-invasive prognostic factor for survival in non-small cell lung cancer

Circulating DNA is a non-invasive prognostic factor for survival in non-small cell lung cancer

Methylation Array Analysis of Preoperative and Postoperative Saliva DNA in Oral Cancer Patients

Extracellular Nucleic Acids and Cancer

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