Can embryo morphokinetic parameters predict euploid pregnancy loss?

McQueen, Dana B.; Mazur, Jenna; Kimelman, Dana; Confino, Rafael; Robins, Jared C.; Bernardi, Lia A.; Chen, Yeh; Zhang, Junyi; Pavone, Mary Ellen

doi:10.1016/j.fertnstert.2020.08.021

Cited by 9 publications

(2 citation statements)

References 20 publications

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“…Additionally, embryo morphokinetics cannot accurately reflect the degree of chromosomal mosaicism [ 15 ]. Even diploid embryos with normal morphological parameters may result in miscarriages [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic signatures in trophectoderm and inner cell mass of human blastocysts classified according to developmental potential, maternal age and morphology

Kai¹,

Mei

Kawano³

et al. 2022

PLoS ONE

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Selection of high-quality embryos is important to achieve successful pregnancy in assisted reproductive technology (ART). Recently, it has been debated whether RNA-sequencing (RNA-Seq) should be applied to ART to predict embryo quality. However, information on genes that can serve as markers for pregnant expectancy is limited. Furthermore, there is no information on which transcriptome of trophectoderm (TE) or inner cell mass (ICM) is more highly correlated with pregnant expectancy. Here, we performed RNA-Seq analysis of TE and ICM of human blastocysts, the pregnancy expectation of which was retrospectively determined using the clinical outcomes of 1,890 cases of frozen-thawed blastocyst transfer. We identified genes that were correlated with the expected pregnancy rate in ICM and TE, respectively, with a larger number of genes identified in TE than in ICM. Downregulated genes in the TE of blastocysts that were estimated to have lower expectation of pregnancy included tight junction-related genes such as CXADR and ATP1B1, which have been implicated in peri-implantation development. Moreover, we identified dozens of differentially expressed genes by regrouping the blastocysts based on the maternal age and the Gardner score. Additionally, we showed that aneuploidy estimation using RNA-Seq datasets does not correlate with pregnancy expectation. Thus, our study provides an expanded list of candidate genes for the prediction of pregnancy in human blastocyst embryos.

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Section: Introductionmentioning

confidence: 99%

Transcriptomic signatures in trophectoderm and inner cell mass of human blastocysts classified according to developmental potential, maternal age and morphology

Kai¹,

Mei

Kawano³

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Many studies have identified parameters associated with improved clinical outcomes, including time of appearance and fading of two pronuclei ( 9 ), the timing of cell cycle events ( 10 , 11 ), the time between 2- to the 8-cell cleavage stage ( 12 , 13 , 14 , 15 ), cleavage events ( 16 ), cytokinesis duration ( 17 ), time to initiation of blastulation ( 18 , 19 , 20 ), and combined morphokinetic and morphologic or molecular parameters ( 21 , 22 , 23 , 24 , 25 , 26 , 27 ). Other studies showed that no differences were found in the time-lapse parameters of euploid embryos that led to miscarriages ( 28 ). Given the divergent findings, site-specific algorithms have been proposed ( 29 ).…”

mentioning

confidence: 97%