Can dried blood spots be used to accurately measure vitamin D metabolites?

Binks, Michael J.; Bleakley, Amy S.; Rathnayake, Geetha; Pizzutto, Susan J.; Chang, Anne B.; McWhinney, Brett; Ungerer, Jacobus P.J.

doi:10.1016/j.cca.2021.03.003

Cited by 7 publications

(8 citation statements)

References 24 publications

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“…Blood samples for vitamin D analysis include both plasma and dried blood spots (DBS). DBS-based 25OHD concentrations will be adjusted for haematocrit as necessary 38. Vitamin D levels <50 nmol/L are considered deficient.…”

Section: Methodsmentioning

confidence: 99%

“…Where volumes allow, blood aliquots will be stored in RNA preservative (RNA later, Invitrogen) for further characterisation of immune effector genes. Circulating calcium levels (via routine pathology services), breast milk vitamin D levels (via an adapted HPLC method)38 and polymorphisms in key vitamin D pathway genes46 (eg, the vitamin D receptor gene, VDR) will also be evaluated. Stool samples are also collected (OMNIgene, DNA Genotek) from a subset of participants for gut microbiome analysis 47 48.…”

Section: Methodsmentioning

confidence: 99%

“…DBS-based 25OHD concentrations will be adjusted for haematocrit as necessary. 38 Vitamin D levels <50 nmol/L are considered deficient.…”

Section: Methodsmentioning

confidence: 99%

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Randomised controlled trial of perinatal vitamin D supplementation to prevent early-onset acute respiratory infections among Australian First Nations children: the ‘D-Kids’ study protocol

Binks,

Bleakley,

Pizzutto

et al. 2023

BMJ Open Resp Res

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IntroductionGlobally, acute respiratory infections (ARIs) are a leading cause of childhood morbidity and mortality. While ARI-related mortality is low in Australia, First Nations infants are hospitalised with ARIs up to nine times more often than their non-First Nations counterparts. The gap is widest in the Northern Territory (NT) where rates of both acute and chronic respiratory infection are among the highest reported in the world. Vitamin D deficiency is common among NT First Nations neonates and associated with an increased risk of ARI hospitalisation. We hypothesise that perinatal vitamin D supplementation will reduce the risk of ARI in the first year of life.Methods and analysis‘D-Kids’ is a parallel (1:1), double-blind (allocation concealed), randomised placebo-controlled trial conducted among NT First Nations mother–infant pairs. Pregnant women and their babies (n=314) receive either vitamin D or placebo. Women receive 14 000 IU/week or placebo from 28 to 34 weeks gestation until birth and babies receive 4200 IU/week or placebo from birth until age 4 months. The primary outcome is the incidence of ARI episodes receiving medical attention in the first year of life. Secondary outcomes include circulating vitamin D level and nasal pathogen prevalence. Tertiary outcomes include infant immune cell phenotypes and challenge responses. Blood, nasal swabs, breast milk and saliva are collected longitudinally across four study visits: enrolment, birth, infant age 4 and 12 months. The sample size provides 90% power to detect a 27.5% relative reduction in new ARI episodes between groups.Ethics and disseminationThis trial is approved by the NT Human Research Ethics Committee (2018-3160). Study outcomes will be disseminated to participant families, communities, local policy-makers, the broader research and clinical community via written and oral reports, education workshops, peer-reviewed journals, national and international conferences.Trial registration numberACTRN12618001174279.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Randomised controlled trial of perinatal vitamin D supplementation to prevent early-onset acute respiratory infections among Australian First Nations children: the ‘D-Kids’ study protocol

Binks,

Bleakley,

Pizzutto

et al. 2023

BMJ Open Resp Res

Self Cite

View full text Add to dashboard Cite

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“…Two biological sample types, blood and saliva, both carrying natural large proteins and complex macromolecules, were selected to study the sensor response. In cases where conventional blood sampling is challenging or considered invasive, dried saliva spots and dried blood spots can provide a practical alternative for collecting and studying samples [ 84 , 85 ]. Dried spot tests can be collected remotely and studied later in the laboratory, in cases where a portable Raman spectrometer is not available.…”

Section: Methodsmentioning

confidence: 99%

Rapid Detection of Amitriptyline in Dried Blood and Dried Saliva Samples with Surface-Enhanced Raman Spectroscopy

Boroujerdi

Paul

Abdelkader

2022

Sensors

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There is growing demand for rapid, nondestructive detection of trace-level bioactive molecules including medicines, toxins, biomolecules, and single cells, in a variety of disciplines. In recent years, surface-enhanced Raman scattering has been increasingly applied for such purposes, and this area of research is rapidly growing. Of particular interest is the detection of such compounds in dried saliva spots (DSS) and dried blood spots (DBS), often in medical scenarios, such as therapeutic drug monitoring (TDM) and disease diagnosis. Such samples are usually analyzed using hyphenated chromatography techniques, which are costly and time consuming. Here we present for the first time a surface-enhanced Raman spectroscopy protocol for the detection of the common antidepressant amitriptyline (AMT) on DBS and DSS using a test substrate modified with silver nanoparticles. The validated protocol is rapid and non-destructive, with a detection limit of 95 ppb, and linear range between 100 ppb and 1.75 ppm on the SERS substrate, which covers the therapeutic window of AMT in biological fluids.

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“…A comparative study of DBS and their matching serum samples from 98 women (who were healthy or were diagnosed with Hashimoto's thyroiditis) showed that DBS can be a suitable alternative to serum, simplifying the sample collection process (Rola et al, 2021). Recently, Binks et al (2021) presented an LC-MS/MS method with PTAD derivatization to determine 25(OH)D 3 , 3α-25(ΟΗ)D 3 and 25(OH)D 2 in DBS. The authors underlined the difficulty of using DBS samples in quantitative applications, since the hematocrit correction was sometimes not able to reflect the real bias between DBS samples and plasma samples.…”

Section: Biological Samples For Vitamin D Analysis Beyond Serum/plasmamentioning

confidence: 99%

Analysis of vitamin D metabolic markers by mass spectrometry: Recent progress regarding the “gold standard” method and integration into clinical practice

Αλεξανδρίδου

Schorr

Stokes

et al. 2021

Mass Spectrometry Reviews

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Liquid chromatography/tandem mass spectrometry is firmly established today as the gold standard technique for analysis of vitamin D, both for vitamin D status assessments as well as for measuring complex and intricate vitamin D metabolic fingerprints. While the actual mass spectrometry technology has seen only incremental performance increases in recent years, there have been major, very impactful changes in the front‐ and back‐end of MS‐based vitamin D assays; for example, the extension to new types of biological sample matrices analyzed for an increasing number of different vitamin D metabolites, novel sample preparation techniques, new powerful chemical derivatization reagents, as well the continued integration of high resolution mass spectrometers into clinical laboratories, replacing established triple‐quadrupole instruments. At the same time, the sustainability of mass spectrometry operation in the vitamin D field is now firmly established through proven analytical harmonization and standardization programs. The present review summarizes the most important of these recent developments.

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Can dried blood spots be used to accurately measure vitamin D metabolites?

Cited by 7 publications

References 24 publications

Randomised controlled trial of perinatal vitamin D supplementation to prevent early-onset acute respiratory infections among Australian First Nations children: the ‘D-Kids’ study protocol

Randomised controlled trial of perinatal vitamin D supplementation to prevent early-onset acute respiratory infections among Australian First Nations children: the ‘D-Kids’ study protocol

Rapid Detection of Amitriptyline in Dried Blood and Dried Saliva Samples with Surface-Enhanced Raman Spectroscopy

Analysis of vitamin D metabolic markers by mass spectrometry: Recent progress regarding the “gold standard” method and integration into clinical practice

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