Can Cryopreservation in Assisted Reproductive Technology (ART) Induce Epigenetic Changes to Gametes and Embryos?

Sciorio, Romualdo; Manna, Claudio; Fauque, Patricia; Rinaudo, Paolo

doi:10.3390/jcm12134444

Cited by 3 publications

(2 citation statements)

References 168 publications

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“…In the literature, this difference is explained by the impact of the freezing medium with cryoprotectants used to limit the freezing damage. In fact, frozen embryos are preserved in a non-physiological storage fluid, and consequently, epigenetic changes in the embryo take place in order to withstand the hostile environment of the storage medium and freezing in liquid nitrogen [26]. In fresh embryo transfer, birthweight is more often below the third percentile [13], as confirmed by our data.…”

Section: Discussionsupporting

confidence: 80%

Impact of Assisted Reproduction Techniques on Adverse Maternal Outcomes and on the Rate of Hospitalization in Maternal Intensive Care

Collée,

Noel,

Seidel

et al. 2023

Medicina

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Background and Objective: The aim of this retrospective cohort study is to evaluate the impact of assisted reproductive treatment (ART) on adverse maternal outcomes and the rate of hospitalization in maternal intensive care (MIC) in a tertiary university center in Liege, Belgium. Materials and Methods: This is a retrospective cohort study comparing two groups, 6557 patients who achieved pregnancy spontaneously and 330 patients who achieved pregnancy after ART, between January 2020 and December 2022. These patients were followed in the academic obstetrics department of Citadelle Hospital, Liège. The database of the ART center was compared with the database of the delivery unit to determine the cohort of patients who conceived after ART. Adverse maternal outcomes and MIC hospitalization rates were compared with between spontaneous pregnancies and ART groups. ART groups were also compared with each other. Results: The rate of hospitalization in maternal intensive care for patients who achieved pregnancy spontaneously was 12.1%, compared to 17.3% after ART. Comparing the rate of pre-eclampsia, 3.5% of spontaneous pregnancies were complicated by pre-eclampsia, while after ART, 10.9% of patients developed this complication during pregnancy. This rate was higher after IVF (12%) compared to intrauterine insemination and particularly after frozen embryo transfer (FET) in artificial cycle (17.9%). The birthweight of newborns after ART was also analyzed. A significant difference was obtained when comparing fresh embryo transfer with FET. Conclusions: Our study confirmed that FET in artificial cycle is a risk factor for pre-eclampsia and that fresh embryo transfer is associated with a higher rate of newborns with a lower percentile of birthweight. Our data showed that the rate of MIC hospitalization was significantly higher after ART but did not differ between groups.

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Section: Discussionsupporting

confidence: 80%

Impact of Assisted Reproduction Techniques on Adverse Maternal Outcomes and on the Rate of Hospitalization in Maternal Intensive Care

Collée,

Noel,

Seidel

et al. 2023

Medicina

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“…Cleavage-stage embryos can be frozen directly, while blastocysts need to be manually shrunk prior to vitrification. It remains unclear whether this difference will yield potential consequences for the birth weight of future offspring ( 30 , 31 ). Therefore, we cannot rule out the possibility that the higher birth weight is due to a greater effect of the vitrification-thawing process itself on the blastocyst compared to the cleavage-stage embryo, and a randomized study of Day 3 versus Day 5/6 using fresh embryo transfer would be necessary.…”

Section: Discussionmentioning

confidence: 99%

The effect of an extended culture period on birth weight among singletons born after single or double vitrified embryo transfer

Wang,

Lin,

Zhao

et al. 2024

Front. Endocrinol.

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AimTo evaluate the effect of an extended culture period on birth weight among singletons born after vitrified-warmed embryo transferMethodsA retrospective cohort study was performed among 12400 women who gave birth to 1015, 1027, 687, and 9671 singletons after single blastocyst transfer, single cleavage-stage embryo transfer, double blastocyst transfer, and double cleavage-stage embryo transfer, respectively.ResultsThe unadjusted birth weight of singletons born after vitrified blastocyst transfer were heavier than those born after cleavage-stage transfer (β=30.28, SE=13.17, P=0.022), as were the adjusted birth weights (β=0.09, SE=0.03, P=0.007). In addition, there was a 37% increased odd of having an infant with high birth weight after vitrified blastocyst transfer compared with vitrified cleavage stage transfer (OR=1.37, 95% CI:1.07-1.77).ConclusionThe unadjusted and adjusted birth weight and odds of having an infant with high birth weight significantly increased after blastocyst transfer compared with cleavage-stage embryo transfer in vitrified-warmed cycles.

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Cleavage-stage or blastocyst-stage embryo biopsy has no impact on growth and health in children up to 2 years of age

Belva,

Kondowe,

De Vos

et al. 2023

Reprod Biol Endocrinol

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Background Studies show conflicting results on neonatal outcomes following embryo biopsy for PGT, primarily due to small sample sizes and/or heterogeneity in the timing of embryo biopsy (day 3; EBD3 or day 5/6; EBD5) and type of embryo transfer. Even fewer data exist on the impact on children’s health beyond the neonatal period. This study aimed to explore outcomes in children born after EBD3 or EBD5 followed by fresh (FRESH) or frozen-thawed embryo transfer (FET). Methods This single-centre cohort study compared birth data of 630 children after EBD3, of 222 EBD5 and of 1532 after non-biopsied embryo transfers performed between 2014 and 2018. Follow-up data on growth were available for 426, 131 and 662 children, respectively. Results Embryo biopsy, either at EBD3 or EBD5 in FET and FRESH cycles did not negatively affect anthropometry at birth, infancy or childhood compared to outcomes in non-biopsied FET and FRESH cycles. While there was no adverse effect of the timing of embryo biopsy (EBD3 versus EBD5), children born after EBD3 followed by FET had larger sizes at birth, but not thereafter, than children born after EBD3 followed by FRESH. Reassuringly, weight and height gain, proportions of major congenital malformations, developmental problems, hospital admissions and surgical interventions were similar between comparison groups. Conclusion Our study indicated that neither EBD3 nor EBD5 followed by FRESH or FET had a negative impact on anthropometry and on health outcomes up to 2 years of age.

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Can Cryopreservation in Assisted Reproductive Technology (ART) Induce Epigenetic Changes to Gametes and Embryos?

Cited by 3 publications

References 168 publications

Impact of Assisted Reproduction Techniques on Adverse Maternal Outcomes and on the Rate of Hospitalization in Maternal Intensive Care

Impact of Assisted Reproduction Techniques on Adverse Maternal Outcomes and on the Rate of Hospitalization in Maternal Intensive Care

The effect of an extended culture period on birth weight among singletons born after single or double vitrified embryo transfer

Cleavage-stage or blastocyst-stage embryo biopsy has no impact on growth and health in children up to 2 years of age

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